Team:The Agency Escondido/notebook

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April 9

Andrew Buss prepared 120 mL of LB broth

April 10

Andrew Buss prepared two LB plates without antibiotics, using our LB plate protocol, rev 72.

Andrew Segina inoculated two ampicillin/kanamycin LB plates with E. coli containing Parts A and B from the 3A assembly kit

Andrew Buss inoculated an SOB plate with NEB10 competent E. coli

April 11

Andrew Segina inoculated an SOB plate with NEB10 competent E. coli

The plates previously inoculated with E. coli with parts A and B do not show visible growth

Andrew Segina prepared 1 L of YPD broth without dextrose

April 12

Andrew Buss inspected plates at 8:15 AM. Both NEB10 plates showed visible growth. Neither plate with Part A or B displayed growth.

Andrew Buss prepared four YPD plates from the 1L stock and mixed 4 grams of table sugar before pouring

Andrew Buss inoculated two LB tubes each with E. coli with parts A and B directly from the agar stabs included in the 3A assembly kit. The intention was to diagnose the lack of growth on the A and B plates over two days.

Andrew Buss inspected plates again around 1:00 PM. NEB10 plates showed additional growth, and the plate with Part A contained visible colonies. The plate with Part B did not show growth, however.

Grant Hassinger mixed 100 mL of LB broth and began an autoclave cycle with the media

Vansh Singh inoculated a tube of ampicillin/kanamycin LB broth from the 3A assembly kit with a colony from the Part A plate

Vansh Singh prepared a tube of ampicillin/kanamycin LB broth from the 3A assembly kit without inoculation. The tube was placed into the refrigerator

Andrew Buss inoculated a tube of LB broth with a colony from the NEB10 plate from April 10

April 13 labeled media

Andrew SeginaLB PlatePart A from kitAmpicillin, kanamycin
Andrew SeginaLB PlatePart B from kitAmpicillin, kanamycin
Andrew BussSOB PlateNEB 10 from kit
Andrew SeginaSOB PlateNEB 10 from kit
Andrew BussLB TubePart A from kitIncubating in dry bath
Andrew BussLB Tube x2Part A from kitIncubating in dry bath
Andrew BussLB Tube x2Part B from kitIncubating in dry bath
Vansh SinghLB TubePart A from plateIncubating in dry bath
Andrew BussLB TubeNEB 10 from 4/10 SOB plateIncubating in dry bath

April 13 available media

Andrew Segina800 mL YPDLacks dextrose
Andrew Buss4 YPD plates
Andrew Segina8 LB plates
Andrew Buss~40mL LB broth
Vansh Singh~5mL LB brothAmpicillin, kanamycin
Grant Hassinger100mL LB broth
Grant Hassinger100mL LB broth