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From 2013hs.igem.org

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Notebook

And if not now, Then when? (Pirkei Avot 1:14)

Our team continuously communicates via frequent Facebook messages, e-mails, and text messages to lively exchange thoughts and ideas; also, we visit CSBL@KU lab about once a week to meet our advisor and do labs.

Brainstorming and Planning

2013-02-13

Eugenie, Gyeongmin, Sirwoo and Joon Hyuck visited the lab to talk to our advisor about the topic of our iGEM project. The following are the ideas that we came up with. Each of them are elaborated on the "Brainstorming" page.

- vanilla-flavor-emitting Lactobacillus, which can be used to make vanilla-flavored yogurt

- inhibition of biofilm-formation of Streptococcus mutans

- CO detecting bacteria

- CO2 fixation bacteria

- bacteria that produces ATP with light, using proteorhodopsin

- plastic degrading bacteria

After the meeting, we did some further search on more information related to the possible topics mentioned above and received feedback from our instructor and advisor.

2013-02-28

Eugenie, Gyeongmin, Joon Hyuck and MyungGun visited the lab.

Before the meeting, through e-mail exchanges and Facebook chats, we had consolidated the direction of our project into constructing bacteria equipped with proteorhodopsin. Our advisor had sent us several theses about this topic; we tried to come up with good ideas on how to apply proteorhodopsin in making the bacteria to perform certain function. We came up with some ideas, such as enhancing butanol production in bacteria or enhancing lactic acid production in Lactobacillus. Later on, our instructor introduced us with 2010 Collegiate iGEM Team Cambridge Project, and suggested to try improving the parts constructed by Team Cambridge by adding gene that expresses proteorhodopsin or by adding other gene that enhances ATP production of E. coli. He also introduced us with [http://www.instructables.com/id/DIY-BioPrinter/ DIY BioPrinter] and suggested to create similar BioPrinter that uses bioluminescence proteins expressed by engineered E. coli instead of ink.

During the meeting, we discussed about the mechanism of DIY BioPrinter that we are planning to construct. We came up with two different mechanisms:

1. Using the bioluminescence protein-expressing bacteria itself as ink

2. Using inducers, such as arabinose and IPTG, as ink and printing on agar plate on which bacterial colonies that expresses bioluminescence proteins when contacted with inducers

The iGEM kit has arrived at the lab a few days ago, so we ook a look at it. We learned how to inoculate bacteria into solid and liquid badge, using the sample E. coli (Part A and Part B) that were included in the kit.