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Team:Shenzhen SFLS/Project
From 2013hs.igem.org
- a team description
- project description
- safety information (did your team take a safety training course? were you supervised in the lab?)
- team attribution (who did what part of your project?)
- lab notebook
- sponsor information
- other information
Example: 2013hs.igem.org/Team:Deerfield_MA/Our_Pets
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| Team Shenzhen_SFLS |
| Official Team Profile |
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Contents |
Team
Peilin Li: the instructor of SFLS students.
Kang Kang: the advisor of SFLS students.
Hekang Jia: the leader of SFLS students.
Biwei Zheng: the vice leader of SFLS students.
Project
Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP, students could simply order it or extract it from a plant. For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does. This can be achieved by:
１．Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast genome.
２．Order this gene from a laboratory．
３．Insert this gene into e。coil or yeast.
４．Isolate AP after it has been expressed.
５． Use fluorometric ａｎａｌｙｓｉｓ ａｎｄ ｎｏｔｅ ｔｈｅ baseline ｗａｖｅｌｅｎｇｔｈoｆｌｉｇｈｔ ｓｈｏｔ ｔｈｒｏｕｇｈ ａ agarose／jellose／ＡＰ in the presence of an acid.
Add different heavy metals or pesticides to this mixture and do other fluorometric analyses. These analyses should yield different wavelengths because AP activity is inhibited by heavy metals, etc.
Notebook
Show us how you spent your days.
Results/Conclusions
What did you achieve over the course of your semester?
Safety
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
Attributions
Who worked on what?
Human Practices
What impact does/will your project have on the public?
Fun!
What was your favorite team snack?? Have a picture of your team mascot?
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