Team:Shenzhen SFLS/Project

From 2013hs.igem.org

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	Team Shenzhen_SFLS

Team

Peilin Li: the instructor of SFLS students.

Kang Kang: the advisor of SFLS students. In BGI, K2 is veeeeery famous for lurking on BBS and sarcasm! As a former champion in a national robot contest, a hacker, a web engineer, a designer, a magazine editor, a citizen reporter, a filmmaker and a drama actor, he has always too many wonderings, including how life works on its core database - genome. Cracking and hacking the code of life, making actors in cell perform on his scripts, has become the primary task of this young scientist.

Hekang Jia: the captain of SFLS students, set his sights on get the only grand prize. The boy who set up the team by himself，has strong desire to make everthing best. He was often very serious in team's seminar and made others under pressure. He is the also the leader of team's students working on theoretical derivation.

Biwei Zheng: the vice captain of SFLS students.

Ruping Liu: Conscientious and careful to anything. She’s interested in biology, not only because of its diversity but also its mystery. Her dream is to do something which is useful to the world in the future. The girl is working on reading paper and do some experiments in SFLS iGEM team.

Kang Li: Eric Lee is a senior one student in SFLS and is responsible for the team’s theoretical group. He has a strong desire in biology and he is very good at communicating with people. So he is also responsible for communicating with the committee. He is a good speaker too. Eric is looking forward to helping the team more.

Ziying Tan: Tina is studying in SFLS Class 7 Grade 10. Aside from spending time before the computer or in the lab, she enjoys rubber sculpture and reading.She is now one of the leaders of vacation club. She couldn`t think of a better way to enjoy her summer in school than joining Shenzhen SFLS IGEM team!

Project

Student at SFLS want to use an enzyme known as “polyphosphate kinase”（PPK）to governance the eutrophication of water bodies. We know that Inorganic phosphate (Pi) is recognized as one of the major nutrients contributing to the outbreak of red tide.We have found that PPK is known to digest Inorganic phosphate (Pi). There is a small amount of it in e.coli and we want to copy the gene of PPK in the e.coli to make it more efficient,so the role of PPK will be more obvious Then we will use the GFP to express the result of the digest of PPK.We want to make two device in a gene circut to deal with this problem.

Device 1: We use a limitable promoter which would be limited by high concentrations of Pi, then the GFP in this device won't light and a protein we add in the device won't breed .

Device 2: If the protein didn't breed, the promoter in Device 2 will be induced and the PPK gene will work, it will digest Pi. When the concentration of phosphate become low, devive 2 will stop to digest Pi and the promoter in device 1 will be induced, then the GFP in device 1 will light. That means the work has finished.

( We will put on our picture of gene circuts in few days)

Notebook

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Results/Conclusions

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Safety

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Attributions

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Human Practices

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Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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