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2024-03-28T10:38:28Z
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http://2013hs.igem.org/Jamboree/Schedule/Practice_sessions
Jamboree/Schedule/Practice sessions
2013-06-26T13:33:53Z
<p>Limu: </p>
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<div class="main_item"> <!-- High School Jamboree Schedule--> <br />
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<div class="title">Practice Sessions</div><br />
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<p>There will be optional practice sessions on Friday June 28 from 6PM to 9PM in the Stata Center. This is a good opportunity for you to test your presentation equipment (computers, adapters, etc.). You can edit this page (hover at the top left corner, above the iGEM logo), and reserve a spot for your team in the table below. </p><br />
<p>There are a limited number of time slots available on a first-come first-serve basis so choose only one slot. Please keep in mind that there will be teams waiting to use the room after you, so make sure that your practice finishes on time! If you're having trouble reserving a spot, send an email to <b>kitwa (at) igem (dot) org</b> with the time and room you'd like to be scheduled for. </p><br />
<p><i>Note: We cannot match the room that you will ultimately give your presentation in with the practice room. There will NOT be any A/V (audio/visual) support on staff. All classrooms will be unlocked and you should use them and leave them as you found them. You must bring your own computer, adaptor, etc., iGEM HQ will not provide these.</i></p><br />
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<th>Room 32-141</th><br />
<th>Room 32-155</th><br />
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<th>6:00PM - 6:15PM</th><br />
<td>BV_CAPS_Kansas</td><br />
<td>UCL Academy</td><br />
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<th>6:15PM - 6:30PM</th><br />
<td>A-2</td><br />
<td>The Agency Escondido</td><br />
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<th>6:30PM - 6:45PM</th><br />
<td>AUC_TURKEY</td><br />
<td>AnatoliaGreece</td><br />
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<tr class="odd"><br />
<th>6:45PM - 7:00PM</th><br />
<td>A-4</td><br />
<td>MCIT_Indianapolis</td><br />
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<th>7:00PM - 7:15PM</th><br />
<td>NGSS_AEI_TURKEY</td><br />
<td>B-5</td><br />
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<th>7:15PM - 7:30PM</th><br />
<td>A-6</td><br />
<td>Shenzhen_SFLS</td><br />
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<tr class="even"><br />
<th>7:30PM - 7:45PM</th><br />
<td>Lambert GA</td><br />
<td>Shenzhen_SZMS</td><br />
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<tr class="odd"><br />
<th>7:45PM - 8:00PM</th><br />
<td>Jefferson_VA_SciCOS</td><br />
<td>Consort Alberta</td><br />
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<tr class="even"><br />
<th>8:00PM - 8:15PM</th><br />
<td>CSIA SouthKorea</td><br />
<td>B-9</td><br />
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<tr class="odd"><br />
<th>8:15PM - 8:30PM</th><br />
<td>A-10</td><br />
<td>Beijing HDFLS</td><br />
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<tr class="even"><br />
<th>8:30PM - 8:45PM</th><br />
<td>A-11</td><br />
<td>B-11</td><br />
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<th>8:45PM - 9:00PM</th><br />
<td>A-12</td><br />
<td>B-12</td><br />
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Limu
http://2013hs.igem.org/Project.htm
Project.htm
2013-06-22T05:21:39Z
<p>Limu: </p>
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<td><p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nitrate (NO3-) and nitrite (NO2-) are chemical roots of nitric acid (HNO3) and nitrous acid (HNO2) respectively. They exist in the aquatic environment, the living organisms and artificial products, as pollutants, which can cause food poisoning, cancers or even death. It's important to detect and eliminate such chemicals with the efficient methods. Currently, several detection methods have already been in use but all are not entirely harmless. For example, granular Cd grains are recommended for the NO3- determination for fruits and vegetables because they are easy to make and recover, and can be used repeatedly. However, Cd cannot be recycled easily, and may cause additional pollution. So the goal of our project is to provide an efficient and a more environment friendly method of detecting nitrates/nitrites and we strive to design biosensors to avoid the disadvantages of traditional methods.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Specific details of the pollution of the Nitrites and Nitrates:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nitrate (NO3-) and nitrite (NO2-) are Chemical roots of nitric acid (HNO3) and nitrous acid (HNO2). They exist in nature, especially in the gaseous water, surface water and groundwater, as well as plants and animals and food within, as environmental pollutants. <br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;There exist a lot of pollution sources of nitrate and nitrite in the environment, such as: <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. Artificial fertilizers: ammonium nitrate, calcium nitrate, potassium nitrate, sodium nitrate and urea;<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Sewage, garbage and human and animal feces: according to tests, 1 liters of domestic sewage in the natural degradation process can produce 110 mg of nitrate; 1 kg of garbage and fecal compost under natural conditions can produce 492 mg of nitrate leaching decomposition;<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. Large amounts of ammonia which food, fuel or oil refining factories emit in waste: after nature biological, chemical conversion cause the formation of nitrate into the environment; <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;4. Cars, trains, ships, aircraft, boilers, civil furnace combustion of petroleum fuels, coal, natural gas: can produce large amounts of nitrogen oxides. Average burning one ton of coal, of 1 kiloliter oil and 10,000 cubic meters of natural gas can produce nitrogen dioxide gas, respectively 9.13 and 63 kg. These nitrogen dioxide gas can be formed after precipitation leaching nitrate descend to the ground and waters ; <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;5. Food preservation and preservation: nitrate and nitrite are widely used in corrosion protection and preservation of meat and fish, so that the meat is red and aroma. Adding sodium nitrate per kilogram of meat in food (usually sodium nitrite) 5 mg or less, within a certain time will percept a good flesh color ; added 20 mg or more, and may exhibit the needed stability in the commercial color; added 50 mg of a special smell.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;These chemical fertilizers in irrigation, sewage, garbage and fecal, industrial nitrogenous waste, fuel combustion, emissions containing nitrogen gas and other pollutions, after precipitation leaching and decomposition in natural conditions, will form nitrate into the river, the lake and into the ground, resulting in nitrate pollution of surface water and groundwater. Infiltration such as sewage, sewage irrigation and unrestrained use of fertilizers sharp increase in groundwater nitrate content can be from a few mg / l to 400 mg / l or more (the national life of the nitrate content of drinking water health standards less than 88.6 mg / l to nitrogen of less than 20 mg / l); indiscriminate use of fertilizers, irrigation, nitrate pollution of water irrigation cause crops absorb large amounts of nitrates, such as over-fertilization spinach production per kilogram dry weight containing nitrite 3600 mg of salt. Moreover, pickled sauerkraut, vegetables after the lon&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;g-distance transport and long-term storage, as well as overnight cooked vegetables are all unhealthy. Because not only a large number of nitrate content increased, but also in the role of nitrate-reducing bacteria, nitrates are reduced to nitrites.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Large amounts of nitrate and nitrite contain in drinking water, vegetables, grain, fish, meat, sauerkraut, overnight cooking, allow direct poisoning by human consumption. Nitrate in the human body can also be reduced to nitrite. Nitrite reacts in human blood; cause the formation of methemoglobin, so that the blood loss of oxygen functionality makes oxygen poisoning. The symptoms caused by it such as dizziness on light, palpitations, vomiting, lips cyanosis, severe cases, unconsciousness, convulsions, shortness of breath, can be life threatening if not rescued in time. Moreover, nitrite may react with secondary amines and from nitrosamines in the environment both in and out human bodies, which will become carcinogenic, teratogenic and mutagenic substances if reaches a certain dose, this will totally do great harm to human health.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In order to prevent the hazard of nitrate and nitrite, in addition to achieve scientific and rational application of fertilizer, prohibit the use of sewage irrigation ,implement the manure treatment of sewage and garbage and other environmental protection measures to protect surface and ground water sources from nitrate and nitrite nitrate pollution, human should also keep a minimum to eat pickled, smoked, wax system of meat, canned food, stains sauerkraut, and salted vegetables; does not buy foods that were kept too long, lethargic or tertian; finish the food right after buying them; does not eat overnight cooked vegetables or keep tertian boiled vegetables; and remember not to confuse the industrial nitrite with normal salt for eating.<br><br />
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<td><p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Introduction:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Our team is going to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia coli (E.coli) chassis. To introduce the whole process of our project, we split our works into 3 parts.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Design</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Firstly, we aim to build a part containing the nitrates/nitrites sensing promoter and the most visible and observable reporter. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This can also be called a development from previous work of Edinburgh 2009. Their aim was to enable E.coli to report the existence of nitrates/nitrites with green fluorescent protein (GFP) and the PyeaR promoter (the sensor of the nitrates/nitrites they created), but we found several drawbacks of their work. Firstly, the GFP reporter within the bacteria is not efficient enough to respond to the inducer so only dim green fluorescence can be detected. Also, this biosensor cannot be recycled for repeated usage because it does not have an efficient way to turn off the reporting process after the detection. Thus, our team aims to improve the biosensor by taking addition of a more remarkable and observable reporter. From the parts registry, we eventually find a possible reporter, the CrtEBI, that, when synthesized, will lead the E. coli to release a specific pigment, lycopene, as the visible color to determine the existence of nitrates/nitrites. Simultaneously, we will incorporate another reporter, the banana odor enzyme (ATF1) generator, which enables our biosensor to produce an aroma of bananas with the existence of nitrates/nitrites. Therefore, our biosensor containing dual reporters may ensure a high accuracy in the detection of nitrates and nitrites.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Secondly, our team planned to build another part that is functional to eliminate the effect given by part 1. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The first step that we took in this plan was finding a reporter in the registry that can cause the degradation of the pigment. More specifically, the proteins produced by this appropriate reporter are useful to degrade the pigment, so that after easily adding certain promoter to control it, this part 2 that we were willing to build could restore the bacteria to the original state. But the fact was crucial. We could not find this reporter at any sources that we were capable to reach. Based on this result, we started step 2, another solution that might enabled us to finish our design. According to the Web sources of the Peking University 2010 (the creator of the CrtEBI), we learned that the pigment, lycopene would be naturally degrade by bacteria in an appropriate acidic environment or under high temperature. Thus we aimed to find any reporter or protein that could produce heat or acid. Although there were some of the proteins in this range, we could not choose them because of the indeterminacy of their experiment, and we failed again as a result. But in the final step, we eventually thought of a more effective and simple method of this part. We planned to find a part that can reduce the expression of the proteins. According to the description of the CrtEBI, which produces enzymes that convert colorless farnesyl pyrophosphate to red lycopene, the only thing we need to do in this part is to enervate the enzymes. Although the pigment still would not disappear, the bacteria would stop producing the pigment and thus it is convenient for us to eliminate the remaining pigment by changing PH or temperature because of the part 2 that we made. <br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Thirdly, our team aimed to enhance our biobricks by designing a color gradient system which can mark the various concentrations of the nitrates/nitrites.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The overall design of the color gradient system is to repeatedly add different color (nestification) to reveal the different molarities of the nitrites and nitrates. Based on the work by Peking University, we found several different colors with the same function as the CrtEBI. Such as the BBa_K274002,BBa_K274001.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To give a simple example, If we linked the PyeaR promoter , the color reporter 1 (we assume that it is red) and a protein reporter ( we assume that it is TetR reporter) together as the first part, then linked the color reporter 2 ( assumed that it is green) and a TetR negative promoter together as the second part, we could probably observe that the color change from green to grey (as the red color was added) when the concentration of nitrites and nitrates was increasing. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To finish this part 3, the most important work for us is to create an appropriate model as measurement of the input, the nitrites and nitrates, and the output, the variable colors. <br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>MORE DETAILS</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Promoter:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We choose the the PyeaR promoter because it is the only suitable one as the essence of our project obviously. It can be found easily on the parts registry: BBa_K216005<br />
Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions. It was built based on the work of Edinburgh 2009.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Reporter:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The CrtEBI which can cause the bacteria to produce the red pigment, lycopene is suitable for our team to replace the conventional part, GFP. It can be also found in parts registry: BBa_K274100<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Inside the reporter, together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a colored signal output, e.g. for biosensors.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This design has been created with a list of other different colorful designs. For example, a related Composite Biobrick is Part BBa_K274200 (CrtEBIY with rbs), which "goes on" one step after lycopene, converting red lycopene to orange beta-carotene pigment.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The banana odor enzyme (ATF1) generator was a standard reporter given by MIT 2006. Many other projects has used this design as a remarkable reporter. It can be found in the parts registry: BBa_J45199<br><br />
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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2013hs/7/7f/Part_01.jpg"></p><br />
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<td><p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Outcome If we can finish the entire plan hopefully, we are willing to have such these result.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;By inserting the first system that we built into the bacteria, we will receive a sensor which, after absorb the nitrites or nitrates, can efficiently produce the remarkable symbols such as pigments and banana odor.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;By inserting the second system into the bacteria, we can let the bacteria be capable to clean all the products it produced previously, so that the target of our experiment can be reused.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;By inserting the final system of our project, we can create a sensor which will produce a color gradient after approaching to the nitrites and nitrates. From this goal we are willing to reach, we can observe the visual change of the bacteria easily, and simultaneously receive a beautiful and cheerful ending of our whole project in the IGEM competition.<br><br />
SZMS-iGEM Protocol<br><br />
0. First of all, be prepared for the plates.(Resistance selection medium ,sterilization)<br><br />
Part One Kitting and Incubating( LEAST 2+12 hours)<br><br />
1. Find parts we want from 384 panel in the refrigerator. The parts we found and decided to use were 1-18C (BBa_J23100) and 2-2I (BBa_J23111), which were not used.<br><br />
2. Inside the plate there were DNA powder, we used tips of trace extractor to puncture aluminum foil, and draw 10μL double-distilled(DD) water with the trace extractor. dissolve DNA into solution. suck the solution again, put into the Polypropylene centrifuge tube (before this we should always write its tab on)<br><br />
---------------------------------------------------15 minutes waiting---------------------------------------------------<br><br />
3. Get the competent cell from the -73C fridge with the commending of our mentors.<br><br />
4. Draw the DNA solution into the tube of E.coli (by trace extraction) 2μL out of 10μL, and put it inside the ice box for 30 minutes.<br><br />
--------------------------------------------------30 minutes waiting----------------------------------------------------<br><br />
5. Turn on the Thermomixer comfort 10min before using.<br><br />
6. Put E.coli tube into Thermomixer comfort for 42 Celsius degree and 90 seconds “Hot Shock”.<br><br />
-------------------------------------------------90 seconds waiting------------------------------------------------------<br><br />
7. Put E.coli back into the ice-box for 5 minutes.<br><br />
---------------------------------------------------5 minutes waiting-----------------------------------------------------<br><br />
8. Add 0.5mL LB (Luria-Bertani medium) into E.coli tube.<br><br />
9. Get 4 culture medium after the tubes were shaken in Thermostatic oscillation incubator.<br><br />
10. Use spreader to put E.coli on the plate (inside super clean bench and remember to grill the spreader on the alcohol burner before using).<br><br />
11. Take the plates with E.coli outside and start germiculturing them in the Constant temperature incubator (37 Celsius degree).<br><br />
<br><br />
Part Two Selecting and Retaining(LEAST 1+12 hours)<br><br />
12. Take the plates out of the Constant temperature incubator.<br><br />
13. Take the test tube from the super clean bench.<br><br />
14. Add 0.5mL LB and 200X of antibiotic into the tubes.<br><br />
15. Select the strain used in the experiment from the plate (the one which is integral and independent with lucid edge will be the proper choice), and put it into the tubes with tips of trace extractor.<br><br />
16. Clean the super clean incubator, put the plates back into the Constant temperature incubator.<br><br />
17. Put the tubes into the shaker and shake under the temperature of 37 Celsius degree.<br><br />
<br><br />
Part Three Measuring(LEAST 2 hours)<br><br />
18. Drop one μL of buffer on the probe as a blank control group.<br><br />
19. Clean the probe and drop one μL of DNA solution on it.<br><br />
20. Start the procedure.<br><br />
21. Note the concentration showed on the screen.<br />
※The concentration we noted was 260/280: 1.93, 44.2ng/μL<br />
The ideal data of concentration 260/280 is 1.8-2.0<br />
Protein pollution if lower than 1.8 and ion pollution when higher than 2.0<br><br />
<br><br />
Part Four Plasmid Collecting(LEAST 3 hours)<br><br />
22. Select the strain (As 15)<br><br />
23. Retain the backups of the bacteria (Into the Constant temperature incubator)<br><br />
24. Add 500μL of equilibrium liquid BL into the tubes.<br><br />
25. Put the tubes into the centrifugal machine to be centrifuged for 1min in 12000 rpm (Remember to arrange the tubes symmetrically).<br><br />
26. At the same time of executing 25, take 1.5mL of bacteria liquid and centrifuge in the same situation as 25.<br><br />
27. Outwell the effluent and add the turbid liquid (contains RNase and enzyme RNA)<br><br />
28. Add P2 and dissociate.<br><br />
29. Add P3 and precipitate the protein.<br><br />
30. Centrifuge the solutions in 12000rpm for 10 minutes, and absorb the supernatant (contains DNA).<br><br />
31. Repeat 30 but change the time to 40-60 seconds.<br><br />
32. Outwell the effluent and add anhydrous ethanol, centrifuge the tubes for 1 minute in 12000rpm, outwell the effluent. (Repeat this step for one time)<br><br />
33. Elution the solution (add the eluent and wash the tube with buffer).<br><br />
34. Measure the concentration of DNA (Do as Part Three)<br><br />
※The concentration data we got was 2.07<br><br />
<br><br />
Part Five Digestion(LEAST 3 hours)<br><br />
35. Choose the buffer (2-3 μL 10X)<br><br />
36. Add 14μL DD water.<br><br />
37. Add 2μL buffer.<br><br />
38. Add 2μL DNA.<br><br />
39. Add 2μL enzyme (contained in the cone sugar).<br><br />
40. Shake the tubes and put them into the Constant temperature incubator to germiculture under 30 Celsius degree.<br><br />
--------------------------------------------------------2 hour waiting------------------------------------------------------<br><br />
<br><br />
Part Six Glue Preparation(LEAST 2.5 hours)<br><br />
41. Decide the size of Gum care and Comb.<br><br />
42. Weigh the agarose. (The proportion can be 1.0% or 1.5%, depends on the experimental need and the size of glue).<br><br />
43. Solute the agarose with TAE.<br><br />
44. Heat the solution and cool it before using.<br><br />
45. Pour the solution into the Gum care and cool for 30 minutes.<br><br />
---------------------------------------------------30 minutes waiting----------------------------------------------------<br><br />
46. Put the Gum care into the electrophoresis tank.<br><br />
47. Drop the colored DNA into the groove on the glue and switch on the electricity. (Remember to put the glue on the side of cathode.)<br><br />
---------------------------------------------------30 minutes waiting----------------------------------------------------<br><br><br />
48. Color the glue with EB (EB is REAAAAAALLY DANGEROUS, be careful!)<br><br />
49. Scan the glue.<br><br />
<br><br />
Part Seven recycle and extract DNA(LEAST 3 hours)<br><br />
50.follow the kit protocol on the book.<br><br />
<br><br />
Part Eight Linking(LEAST 3 hours)<br><br />
51. Get the EP tubes and make marks on them.(Better the name of DNA)<br><br />
52. Get the glue made in Part Six and put it under the UV-light(remember to wear the safety goggles).<br><br />
※2I: 1.110<br><br />
GFP: 1.082<br><br />
18C: 1,.080<br><br />
GP: 1.086<br><br />
53. Cut the glue with the parts we need.<br><br />
※The weight<br><br />
Total:<br><br />
18C: 1.370<br><br />
GFP: 1.230<br><br />
2I: 1.244<br><br />
6P: 1.345<br><br />
Glue:<br><br />
18C: 290mg*3 870<br><br />
GFP: 148mg*3 444<br><br />
2I: 134mg*3 402<br><br />
6P: 259mg*3 777<br><br />
※the weight of glue: solvent should be 1:3, the solvent is Buffer QG<br><br />
54. Shake the tubes every 2-3 minutes for the duration of 10 minutes under the temperature of 50-60 Celsius degree in order to help dissolving.<br><br />
55. Add isopropanol (as heavy as the glue), transfer the solution into the tubes, centrifuge for 1 minute, outwell the effluent.<br><br />
56. Add PE, centrifuge for 1 minute, outwell the effluent.<br><br />
57. Centrifuge for 1 minute again.<br><br />
58. Add 50 μL DD water into the tubes.<br><br />
59. Centrifuge the tubes again.<br><br />
60. Get the water and DNA ligase.<br><br />
※10μL in total<br><br />
GFP: 2μL, buffer 1μL, enzyme 1μL<br><br />
6P: 2μL, buffer 1μL, enzyme 1μL<br><br />
2I: 2μL, buffer 1μL, enzyme 1μL<br><br />
18C: 2μL, buffer 1μL, enzyme 1μL<br><br />
Polish the solution with DD water.<br><br />
61. Add those elements showed above(enzyme at last).<br><br />
--------------------------------------------------------1 hour waiting------------------------------------------------------<br><br />
Part Nine Transferring.(the same as Part One,LEAST 2+12 hours)<br><br />
62. Get the competent cell from the -73C fridge with the commending of our mentors.<br><br />
63. Draw the DNA solution into the tube of E.coli (by trace extraction) 2μL out of 10μL, and put it inside the ice box for 30 minutes.<br><br />
--------------------------------------------------30 minutes waiting----------------------------------------------------<br><br />
64. Turn on the Thermomixer comfort 10min before using.<br><br />
65. Put E.coli tube into Thermomixer comfort for 42 Celsius degree and 90 seconds “Hot Shock”.<br><br />
-------------------------------------------------90 seconds waiting------------------------------------------------------<br><br />
66. Put E.coli back into the ice-box for 5 minutes.<br><br />
---------------------------------------------------5 minutes waiting-----------------------------------------------------<br><br />
67. Add 0.5mL LB (Luria-Bertani medium) into E.coli tube.<br><br />
68. Get 4 culture medium after the tubes were shaken in Thermostatic oscillation incubator.<br><br />
69. Use spreader to put E.coli on the plate (inside super clean bench and remember to grill the spreader on the alcohol burner before using).<br><br />
70. Take the plates with E.coli outside and start germiculturing them in the Constant temperature incubator (37 Celsius degree).<br><br />
<br><br />
Part Ten Check if we success or not<br><br />
71. Take pictures, collect and arrange data, summary.<br><br />
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<td width="94%" style="font-size:24px;color:#00904b; ">iGEM Safety Questions</td><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1"><h1>&nbsp;</h1><br />
<p><strong>1. Would any of your project ideas raise safety issues in terms of:</strong><br /><br />
<strong>RESEARCHER SAFETY</strong><br /><br />
We have not worked with any materials that raise risk to researchers' safety or health. We only worked with experiments based on commonly used E. coli and baking yeast strains. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely.<br /><br />
Sometime our researchers have to face regular bio-safety challenges in a molecular microbiology lab, such as staining DNA agarose gel with EB, each member of our team is careful to use gloves, goggles, and lab coats in order to protect us .There is also a container for us to pour culture fluid and culture medium full of cells in, and thus it will be treated properly afterwards.<br /><br />
Before the real experiment each member of our team took 2 weeks to receive training. Any researchers conducting experiment were instructed about the right method both by printed protocols and in the lab. After the training, we passed an on-line test about the lab safety, standard experiment procedures and lab waste treatment. During any experiments, necessary individual protections such as gloves and white robes were strictly required. Also, the public safety of the lab is guaranteed by the strict registering protocols on the operation of any apparatus. In order to make sure all the requirements of safety were carried out well by the researchers and to get prepared for any emergency, our lab set up 24hr*7 camera system in the rooms.<br /><br />
<strong>PUBLIC SAFETY</strong><br /><br />
As we said, what we used are non-pathogenic E. coli strains. We will use the experimental plasmid containing antibiotics. So one of the security risks is that antibiotic resistance gene may be transferred to other pathogenic strains and cause the public danger.<br /><br />
To avoid it happening, we gather all the culture fluid and culture medium with cells for unified treatment and not let cells get out of our lab through anyway. <br /><br />
<strong>ENVIRONMENTAL SAFETY</strong><br /><br />
We take much care of the environmental safety. We make sure no media containing engineered cells go into the sewer before sterilization, and all utensil contacted with the engineered bacteria will be sterilized. Toxic and hazardous chemicals are collected and treated properly according to the safety rules of our lab.<br /><br />
<br /><br />
<strong>2. Do any of the new BioBrick parts (or devices) that you made this year raise safety issues?</strong><br /><br />
NO. We don’t think that our new devices will raise safety issues. Most of our BioBricks have been used by former iGEM projects and proved to be innocuous too.<br /><br />
<br /><br />
<strong>3. Is there a local biosafety group, committee, or review board at your institution? </strong><br /><br />
<strong>If yes, what does your local biosafety group think about your project? </strong><br /><br />
<strong>If no, which specific biosafety rules or guidelines do you have to consider in your country? </strong><br /><br />
<strong>BIOSAFETY PROVISIONS</strong><br /><br />
Our experiment is designed and carried out following the standards of national biosafety office, therefore no laws or regulations regarding the biosafety issue are violated.<br /><br />
Because our school (Shenzhen Middle School) has only a brand new laboratory which is established in 2011, so far, we do not have a biosafety committee in our institute. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely. Many teachers and assistants from biology and chemistry department of our school care our safety and have checked the laboratory we used and the processes we have done. And they think the condition we were working is safe and reliable.<br /><br />
<br /><br />
<strong>1.Do you have any other ideas how to deal with safety issues that could be</strong><strong>useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong><br /><br />
<br /><br />
Yes. We suggest the registry of standard biological parts should have a dedicated safety page of every part to ensure that users would not raise any safety issue when using them. And these data should be presented when we are searching for a certain part and found its instructions. By this act, we can at least prevent the safety issues lurking in the shadows before they could appear.<strong> </strong></p></td><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1" valign="top"><p><span class="STYLE7">F</span>irstly, let us show our great appreciation and respect to our four tutors: Li Mu, Kang Kang, Xiao Kang and Liu Yue, without their help on school and daily affairs solving, theory and experiment supporting and the unremittingly attention and encouragement, it can be impossible for us to achieve the fruit now, their every lecture for us, every note writing on our experiment notebook and every meeting held with us together make up our nowadays success.<br /><br />
Also, we should thank the BGI graduate school which bountifully provided us the proper laboratory, advanced equipment and abundant material to complete our experiments and researches. Although how brilliant or impeccable our ideas are, we can’t make them come true without BGI’s resource offering. Not to mention the favorable condition of board and lodging they provided for us during the days we stayed overnight.<br /><br />
And at last, thank for our almamater, Shenzhen Middle School’s supporting and approving on our research, if they didn’t allow us to drop several national classes in completing our experiments, our achievements will also be debarred in papers and our mind instead of the shining light on petri dishes which stands for success.</p><br />
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<td width="94%" style="font-size:24px;color:#00904b; ">Acknowledgments</td><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1" valign="top"><p><span class="STYLE7">F</span>irstly, let us show our great appreciation and respect to our four tutors: Li Mu, Kang Kang, Xiao Kang and Liu Yue, without their help on school and daily affairs solving, theory and experiment supporting and the unremittingly attention and encouragement, it can be impossible for us to achieve the fruit now, their every lecture for us, every note writing on our experiment notebook and every meeting held with us together make up our nowadays success.<br /><br />
Also, we should thank the BGI graduate school which bountifully provided us the proper laboratory, advanced equipment and abundant material to complete our experiments and researches. Although how brilliant or impeccable our ideas are, we can’t make them come true without BGI’s resource offering. Not to mention the favorable condition of board and lodging they provided for us during the days we stayed overnight.<br /><br />
And at last, thank for our almamater, Shenzhen Middle School’s supporting and approving on our research, if they didn’t allow us to drop several national classes in completing our experiments, our achievements will also be debarred in papers and our mind instead of the shining light on petri dishes which stands for success.</p><br />
<p>&nbsp;</p></td><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0402.htm
Notebook/notebook 0402.htm
2013-06-21T19:35:29Z
<p>Limu: </p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 2</td><br />
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<td height="52"><div align="center" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Confirm the project we plan to do. Write the abstract.</div></td><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0409.htm
Notebook/notebook 0409.htm
2013-06-21T19:30:44Z
<p>Limu: </p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 9</td><br />
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<p style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Cut the promoter and other parts.Link them.</p><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0405.htm
Notebook/notebook 0405.htm
2013-06-21T19:30:30Z
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<td><img src="https://static.igem.org/mediawiki/2013hs/b/b7/Notebook_01.jpg" border="0" usemap="#Map" /></td><br />
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<td width="81%" class="STYLE7">&nbsp;</td><br />
<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 5</td><br />
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</table></td><br />
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<td width="89%"><div align="center" class="STYLE6"><br />
<div align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Find the parts we need in case.Devise how to do it.</div><br />
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<table width="66%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td height="123"><img src="https://static.igem.org/mediawiki/2013hs/a/a7/PIC_10.jpg" width="580" height="826" /></td><br />
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<table width="66%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td height="123"><img src="https://static.igem.org/mediawiki/2013hs/e/ec/PIC_12.jpg" width="580" height="676" /></td><br />
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<table width="66%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td height="123"><img src="https://static.igem.org/mediawiki/2013hs/8/82/PIC_11.jpg" width="580" height="826" /></td><br />
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<table width="66%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td height="123"><img src="https://static.igem.org/mediawiki/2013hs/1/19/PIC_13.jpg" width="580" height="826" /></td><br />
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<table width="66%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td height="123"><img src="https://static.igem.org/mediawiki/2013hs/e/e9/PIC_14.jpg" width="580" height="621" /></td><br />
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<td width="5%">&nbsp;</td><br />
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<table width="761" border="0" cellspacing="0" cellpadding="0"><br />
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<td><img src="https://static.igem.org/mediawiki/2013hs/1/16/Notebook_03.jpg" width="747" height="58" /></td><br />
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<td background="https://static.igem.org/mediawiki/2013hs/5/52/Buttom_02.jpg"><table width="10%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td><img src="https://static.igem.org/mediawiki/2013hs/4/4e/Buttom_03.jpg" /></td><br />
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</body><br />
</html></div>
Limu
http://2013hs.igem.org/Notebook/notebook_0402.htm
Notebook/notebook 0402.htm
2013-06-21T19:30:06Z
<p>Limu: </p>
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<div><html><br />
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<td width="116"><a href="home.htm"><img src="https://static.igem.org/mediawiki/2013hs/a/a0/Title_home.jpg" width="116" height="76" / border="0"></a></td><br />
<td width="116"><a href="https://2013hs.igem.org/Team:Shenzhen_SZMS"><img src="https://static.igem.org/mediawiki/2013hs/a/a0/Title_home.jpg" width="116" height="76" / border="0"></a></td><br />
<td width="144"><a href="https://2013hs.igem.org/Project.htm"><img src="https://static.igem.org/mediawiki/2013hs/c/c2/Title-project.jpg" width="144" height="76" / border="0"></a></td><br />
<td width="112"><a href="https://2013hs.igem.org/Team.htm"><img src="https://static.igem.org/mediawiki/2013hs/e/ed/Title_team.jpg" width="112" height="76" / border="0"></a></td><br />
<td width="173"><a href="https://2013hs.igem.org/Notebook/notebook.htm"><img src="https://static.igem.org/mediawiki/2013hs/2/25/Title-notebook.jpg" width="173" height="76" / border="0"></a></td><br />
<td width="254"><a href="https://2013hs.igem.org/Human.htm"><img src="https://static.igem.org/mediawiki/2013hs/4/41/Title_human-practice.jpg" width="254" height="76" / border="0"></a></td><br />
<td width="117"><a href="https://2013hs.igem.org/Jsp/other.htm"><img src="https://static.igem.org/mediawiki/2013hs/7/7d/Title-other.jpg" width="116" height="76" / border="0"></a></td><br />
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</map><br />
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<td height="84" background="https://static.igem.org/mediawiki/2013hs/1/17/Notebook_02.jpg"><table width="100%" height="64" border="0" cellpadding="0" cellspacing="0"><br />
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 2</td><br />
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<td>&nbsp;</td><br />
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<table width="761" border="0" cellspacing="0" cellpadding="0" bgcolor="#F8F8F8"><br />
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<td height="52"><div align="center" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Confirm the project we plan to do. Write the abstract.</div></td><br />
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<tr><br />
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<td width="245"><img src="https://static.igem.org/mediawiki/2013hs/c/c7/Buttom_01.jpg" width="245" height="166" /></td><br />
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</body><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0530.htm
Notebook/notebook 0530.htm
2013-06-21T19:29:32Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="116"><a href="https://2013hs.igem.org/Team:Shenzhen_SZMS"><img src="https://static.igem.org/mediawiki/2013hs/a/a0/Title_home.jpg" width="116" height="76" / border="0"></a></td><br />
<td width="144"><a href="https://2013hs.igem.org/Project.htm"><img src="https://static.igem.org/mediawiki/2013hs/c/c2/Title-project.jpg" width="144" height="76" / border="0"></a></td><br />
<td width="112"><a href="https://2013hs.igem.org/Team.htm"><img src="https://static.igem.org/mediawiki/2013hs/e/ed/Title_team.jpg" width="112" height="76" / border="0"></a></td><br />
<td width="173"><a href="https://2013hs.igem.org/Notebook/notebook.htm"><img src="https://static.igem.org/mediawiki/2013hs/2/25/Title-notebook.jpg" width="173" height="76" / border="0"></a></td><br />
<td width="254"><a href="https://2013hs.igem.org/Human.htm"><img src="https://static.igem.org/mediawiki/2013hs/4/41/Title_human-practice.jpg" width="254" height="76" / border="0"></a></td><br />
<td width="117"><a href="https://2013hs.igem.org/Jsp/other.htm"><img src="https://static.igem.org/mediawiki/2013hs/7/7d/Title-other.jpg" width="116" height="76" / border="0"></a></td><br />
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 30</td><br />
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<p style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Wiki accomplished</p><br />
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<img src="https://static.igem.org/mediawiki/2013hs/6/61/Pic_---.jpg" width="600" height="349" /><br /><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0528.htm
Notebook/notebook 0528.htm
2013-06-21T19:28:26Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="112"><a href="https://2013hs.igem.org/Team.htm"><img src="https://static.igem.org/mediawiki/2013hs/e/ed/Title_team.jpg" width="112" height="76" / border="0"></a></td><br />
<td width="173"><a href="https://2013hs.igem.org/Notebook/notebook.htm"><img src="https://static.igem.org/mediawiki/2013hs/2/25/Title-notebook.jpg" width="173" height="76" / border="0"></a></td><br />
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 28</td><br />
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<p align="center" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Achieve the final aim.Complete the Project.</p><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0519.htm
Notebook/notebook 0519.htm
2013-06-21T19:27:23Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 19</td><br />
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<p align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">The e-coli detect the nitrate successfully.However it is light-colored.<br /><br />
We determined to improve it.</p><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0507.htm
Notebook/notebook 0507.htm
2013-06-21T19:26:26Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 7</td><br />
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<p align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Ensure our uniform and the Team Logo.</p><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0503.htm
Notebook/notebook 0503.htm
2013-06-21T19:25:10Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 3</td><br />
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<p align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Begin to make wiki.<br /><br />
We read a lot about how to make website and ask for professional information.It really make us confused because it’s so difficult to make a great wiki!Fortunately,we accomplish it........</p><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0423.htm
Notebook/notebook 0423.htm
2013-06-21T19:24:05Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 23</td><br />
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<p style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">We begin the Human Practice.We publicity our IGEM project in different high school.making the yogurt.</p><br />
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Limu
http://2013hs.igem.org/Team_02.htm
Team 02.htm
2013-06-21T19:17:12Z
<p>Limu: </p>
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<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
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<td width="92%"><span class="STYLE6"><a href="team_03.htm">Contact us</a></span></td><br />
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<td width="92%"><span class="STYLE6"><a href="team_02.htm">Outtakes</a></a></span></td><br />
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Our human practice!<br />
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<img src="https://static.igem.org/mediawiki/2013hs/1/16/Out_take2.jpg" /><br /><br />
Our experiment note book! How assiduous we are~<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/8/84/Out_take3.jpg" /><br /><br />
Our hero^^<br /><br />
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How beautiful!......at least on me.<br />
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<img src="https://static.igem.org/mediawiki/2013hs/4/40/Out_take6.jpg" /><br /><br />
After a long time boring meeting. A inspiration of our logo!<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/5/5a/Out_take7.jpg" /><br /><br />
Two lovely girls!^^<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/3/3f/Out_take8.jpg" /><br /><br />
Yummy! <br />
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<img src="https://static.igem.org/mediawiki/2013hs/b/b7/Out_take9.jpg" /><br /><br />
Cute loli~~<br /><br />
At last all of us!<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/1/11/Out_take10.jpg" /><br /><br />
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Limu
http://2013hs.igem.org/Team_03.htm
Team 03.htm
2013-06-21T19:16:51Z
<p>Limu: </p>
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<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
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<td width="4%">&nbsp;</td><br />
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<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
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<td width="64%" bgcolor="#F7F7F7">&nbsp;</td><br />
</tr><br />
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<td colspan="2" valign="top" bgcolor="#F7F7F7"><p class="STYLE1"><span class="STYLE9">c</span>ontact us<br />
<br />
we are shenzhen middle school iGEM team!<br /><br />
If you want to contact us, you can log in our school website www.shenzhong.net<br /><br />
or you can send email to lim746@126.com.<br /><br />
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<td>&nbsp;</td><br />
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</td><br />
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Limu
http://2013hs.igem.org/Team_01.htm
Team 01.htm
2013-06-21T19:16:06Z
<p>Limu: </p>
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<td width="673" valign="top"><table width="100%" height="312" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
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<td height="293" bgcolor="#F7F7F7">&nbsp;</td><br />
<td colspan="2" valign="top" bgcolor="#F7F7F7"><p class="STYLE1"><span class="STYLE9">S</span>chool Introduction---Shenzhen Middle School: <br /><br />
&nbsp;Shenzhen Middle School was founded in 1947, and was affirmed as the only provincial key middle school in June 1983, the fast improving and well effective school in 1986, the sample school of the national new curriculum standard in 2004 and one of the first group of schools which pass Cantonese national exemplary high school junior assess in June 2006. Shenzhen Middle School passed the assess of national exemplary school, and precisely raised the mission of “building the technicality high school and fostering innovation-type talents” and the working definition of “building the outstanding academic Chinese high school during the international period”.<br /><br />
&nbsp;The high school club of Shenzhen Middle School was consisted of the two campuses of East and West, it covers the area of 9.8 square kilometers with a collection of 1150 thousand books, and preliminary structures the cyber fundamental facilities and essential service supporting platform with the goal of achieving the digitization, webified and informatization of environment, resource and activity.<br /><br />
&nbsp;At the moment, the high school club has 60 academic classes with students reaching the number of 2872. And those students of Shenzhen Middle School all gets brilliant academic achievement the grade of college entrance examination has the advanced advantage among the whole city and even whole province, the percent of undergraduate course and emphasis school are always keeping still above 90% and 60% since middle of the nineties, and over 200 students successfully entered Beijing and Qinghua Universities which are the best universities in China in the average number of 30 annually.<br /><br />
Shenzhen Middle School also has abundant fruit on international field: it has won nine gold metals of international Olympic competition and over 200 students of it entered the universities in the top 50 of USA.<br /><br />
Furtherly, in order to promote the comprehensive development, foster the wholesome personality and cultivate the aesthetic sentiment of students, besides providing superior studying conditions, Shenzhen Middle School also offers its students affluent after-class life platforms. In Shenzhen Middle School, we can find student organization such as school Youth League Committee, student union, committee of student congress and the senior group; the activities such as soccer games, basketball match, sports festival, culture and art festival, cultural lecture, Shenzhong Forum, subject week and expert lecture; more than fifty student clubs such as chorus, wind band, dance troupe, volunteer team, drama club, astronomy club, parkour club, simulative U.N. and National Middle School Student Panda Protecting Organization. All of these activities and organizations are the important platform for students to attend social practice and develop personalities freely.<br /><br />
Shenzhen Middle School was named as National Artistic Advanced School, National Aesthetic Curriculum Reform Experimental School and Cantonese Artistic Education Exemplary School in 2010 by Ministry of Education. The famous clubs of chorus, wind band and dance troupe has repeatedly won golden awards in international aesthetic competitions and won fourteen national first awards and one second award in only three periods of national artistic exhibition. And noticeably, the chorus of Shenzhen Middle School has won the gold award of Olympic Chorusing Competition for continuously three years.<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/8/82/School.jpg" /><br />
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Limu
http://2013hs.igem.org/Jsp/other_02.htm
Jsp/other 02.htm
2013-06-21T19:15:28Z
<p>Limu: </p>
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1" valign="top"><p><span class="STYLE7">F</span>irstly, let us show our great appreciation and respect to our four tutors: Li Mu, Kang Kang, Xiao Kang and Liu Yue, without their help on school and daily affairs solving, theory and experiment supporting and the unremittingly attention and encouragement, it can be impossible for us to achieve the fruit now, their every lecture for us, every note writing on our experiment notebook and every meeting held with us together make up our nowadays success.<br /><br />
Also, we should thank the BGI graduate school which bountifully provided us the proper laboratory, advanced equipment and abundant material to complete our experiments and researches. Although how brilliant or impeccable our ideas are, we can’t make them come true without BGI’s resource offering. Not to mention the favorable condition of board and lodging they provided for us during the days we stayed overnight.<br /><br />
And at last, thank for our almamater, Shenzhen Middle School’s supporting and approving on our research, if they didn’t allow us to drop several national classes in completing our experiments, our achievements will also be debarred in papers and our mind instead of the shining light on petri dishes which stands for success.</p><br />
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<td width="94%" style="font-size:24px;color:#00904b; ">iGEM Safety Questions</td><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1"><h1>&nbsp;</h1><br />
<p><strong>1. Would any of your project ideas raise safety issues in terms of:</strong><br /><br />
<strong>RESEARCHER SAFETY</strong><br /><br />
We have not worked with any materials that raise risk to researchers' safety or health. We only worked with experiments based on commonly used E. coli and baking yeast strains. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely.<br /><br />
Sometime our researchers have to face regular bio-safety challenges in a molecular microbiology lab, such as staining DNA agarose gel with EB, each member of our team is careful to use gloves, goggles, and lab coats in order to protect us .There is also a container for us to pour culture fluid and culture medium full of cells in, and thus it will be treated properly afterwards.<br /><br />
Before the real experiment each member of our team took 2 weeks to receive training. Any researchers conducting experiment were instructed about the right method both by printed protocols and in the lab. After the training, we passed an on-line test about the lab safety, standard experiment procedures and lab waste treatment. During any experiments, necessary individual protections such as gloves and white robes were strictly required. Also, the public safety of the lab is guaranteed by the strict registering protocols on the operation of any apparatus. In order to make sure all the requirements of safety were carried out well by the researchers and to get prepared for any emergency, our lab set up 24hr*7 camera system in the rooms.<br /><br />
<strong>PUBLIC SAFETY</strong><br /><br />
As we said, what we used are non-pathogenic E. coli strains. We will use the experimental plasmid containing antibiotics. So one of the security risks is that antibiotic resistance gene may be transferred to other pathogenic strains and cause the public danger.<br /><br />
To avoid it happening, we gather all the culture fluid and culture medium with cells for unified treatment and not let cells get out of our lab through anyway. <br /><br />
<strong>ENVIRONMENTAL SAFETY</strong><br /><br />
We take much care of the environmental safety. We make sure no media containing engineered cells go into the sewer before sterilization, and all utensil contacted with the engineered bacteria will be sterilized. Toxic and hazardous chemicals are collected and treated properly according to the safety rules of our lab.<br /><br />
<br /><br />
<strong>2. Do any of the new BioBrick parts (or devices) that you made this year raise safety issues?</strong><br /><br />
NO. We don’t think that our new devices will raise safety issues. Most of our BioBricks have been used by former iGEM projects and proved to be innocuous too.<br /><br />
<br /><br />
<strong>3. Is there a local biosafety group, committee, or review board at your institution? </strong><br /><br />
<strong>If yes, what does your local biosafety group think about your project? </strong><br /><br />
<strong>If no, which specific biosafety rules or guidelines do you have to consider in your country? </strong><br /><br />
<strong>BIOSAFETY PROVISIONS</strong><br /><br />
Our experiment is designed and carried out following the standards of national biosafety office, therefore no laws or regulations regarding the biosafety issue are violated.<br /><br />
Because our school (Shenzhen Middle School) has only a brand new laboratory which is established in 2011, so far, we do not have a biosafety committee in our institute. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely. Many teachers and assistants from biology and chemistry department of our school care our safety and have checked the laboratory we used and the processes we have done. And they think the condition we were working is safe and reliable.<br /><br />
<br /><br />
<strong>1.Do you have any other ideas how to deal with safety issues that could be</strong><strong>useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong><br /><br />
<br /><br />
Yes. We suggest the registry of standard biological parts should have a dedicated safety page of every part to ensure that users would not raise any safety issue when using them. And these data should be presented when we are searching for a certain part and found its instructions. By this act, we can at least prevent the safety issues lurking in the shadows before they could appear.<strong> </strong></p></td><br />
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Limu
http://2013hs.igem.org/Human.htm
Human.htm
2013-06-21T19:14:11Z
<p>Limu: </p>
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<h3>Publicity</h3><br />
<div class="tab"><p><b>human practise</b><br /><br />
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&nbsp;&nbsp;&nbsp;&nbsp;The final plan of our team to propagate our project and the synthetic biology is to disseminate those to the citizens directly. We decided to summarize the main content of our project and print it out, in order to make it accessible for all the citizens to see in the most convenient way. Then we planned to bring all those products to public, the city enter so that we would disseminate them to as much citizens as we can.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;We finished print the booklets before going out to the public. The content of the booklet is simpler than that from our wikis. But it is easier for the citizens to understand. <br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;After discussing, we eventually chose to go to a book city (a huge book shop), a hospital and a estate with many high-tech companies in it. The main groups of people we decide to talk with are students, parents and old men who would probably take more attention to our scientific and biological project.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;This is the visual evidence of our activities of the human practice.<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/7/74/Human_01.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/2/28/Human_02.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/e/e8/Human_03.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/f/f3/Human_04.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/6/61/Human_05.jpg" width="292" height="170"><br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;During our activities, we accepted an interview of a reporter who was interested to put our experience to the newspaper of next day. We are very happy to talk about the details of our project to the reporter and finally successful to explain the main motivation of our project and to illustrate a summary of the synthetic biology and the IGEM competition.<br /><br />
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&nbsp;&nbsp;&nbsp;&nbsp;<b>Our project was reported by the local newspaper.</b><br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/d/d9/Human_06.jpg" width="292" height="170"><br><br />
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Translation:<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;We were caught by some students of Shenzhen Middle School.According to their words,they invent a e-coli which can detect the Nitrite.They also publicized their manual with the detailed project.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;‘Nitrite is everywhere. It can harm people’s health through the way we can’t be aware of ’ Said by Shenzhen Middle School’s students.In order to prevent it.They transform an e-coli. They put genes that can be aware of the Nitrite and link a special alarm after the gene. When it meet the Nitrite,it can touch off the alarm so that, people can detect the Nitrite straightly! Citizens said.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;When we ask them about the experiment, they say ‘It’s very difficult.’In the laboratory, they can’t finish the experiment with their senior high’s knowledge.Not only learn the knowledge,but also practice their skill.All their result now is combined of tears and fortune.<br /><br />
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<h3>Presentantion</h3><br />
<div class="tab wushi"><p>Lectures on Synthetic Biology <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;School visit is part of our project that could directly inform the high school students about synthetic biology. We visited Yaohua High School, Sixth High School and Nanshan School affiliated with China National Institute for Educational Research(SZyangxiao). And we gave a presentation on the Synthetic Biology and iGEM. Looking down on the faces of these students, we knew that some of whom may end up doing biological research a few years later.<br><br />
Yaohua High School<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;We introduced the conception of Synthetic Biology and showed some slices about the harmful effect of nitrate and nitrite. Then we presented iGEM and our project to them. We also answered some questions asked by the curious senior school students. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Synthetic biology is still in an early developing stage in China and it is believed to bring new opportunities to biotechnology and to solve critical problems and scares that we might face now and in the future, like food and fuel crisis. However, similar to the situation in western countries, synthetic biology is still in an emerging field in China that lacks clear definition and safety laws. The lack of a well-accepted definition and safety concerns do not seem to stop the Chinese government and scientists from pursuing synthetic biology. In recent years, high standard labs and institutions have been set up featuring mainly on research of synthetic biology. Funds from Chinese government on synthetic biology increases significantly. There are several universities that are playing the leading role in the field of synthetic biology in China, while more and more universities and colleges are joining in. A good example to show the Chinese students’ curiosity on synthetic biology might be the increasing number of universities that took part in the iGEM competition through the past five years.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Our lectures and discussion sessions were open to anyone who wanted to join. Because the class was for people of all experience levels, our lecture started as an explanation of the most important biological mechanisms. Building on the basics, then we introduced synthetic biology both within the context of iGEM and as it is understood in some of the most significant synbio laboratories. When we felt like the basics of synthetic biology had been covered, we were keen to explore its public perception as well as the possibilities of its future. We involved discussion groups on topics from the ethics of synthetic biology to its public image to the problem of intellectual property. <br><br />
The sixth high school<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;To popularize the concept and spirit of synthetic biology,SZMS iGEM team held a lecture on June 11th, which was mainly oriented to the grade 10 students in The Sixtn High School, as well as all the other students who were interested in synthetic biology.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;The preparation of the lecture started 3 weeks prior to the lecture. Posters were elaborately designed and posted up around the campus. We also bulletined the news through E-mail and phone calls, to ensure every potential audience might be interested in it and took part in.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;On the afternoon of June 11th, we were flattered the moment walking into meeting place, for the room was almost filled up. Never had we thought that the lecture would attract so many audiences. The audiences, most of which were grade 10 students, responded warmly to the splendid lectures given by the three speakers. Chen Pengxuan first gave an overall introduction of the history of synthetic biology. And then he went on with several detailed programs and potential applications on theory of synthetic biology. The audiences were shocked by the marvelous and multiple work done by the engineered bacteria in a popular and easy-to-understand language. The talks took ninety minutes in all, while the question and answer session lasted for another half an hour. His wonderful speech in his unique humorous style won him warm applause from the audiences in the end.<br><br />
SZyangxiao<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;In order to promote synthetic biology and iGEM, we decided to give a presentation to a senior school, SZyangxiao. During the presentation, we give them a brief introduction of the basic knowledge,research strategy , application and industrialization of synthetic biology. Furthermore, we also introduced several interesting iGEM projects. In the end of the presentation, we introduced our iGEM project this year in a plain way. With the help of the presentation and their basic knowledge on the synthetic biology, they have a very clear idea about what iGEM is like as well as our project when we finished.<br> <br />
&nbsp;&nbsp;&nbsp;&nbsp;As synthetic biology is going to be the next big thing in biology, its researches are always raising public concerns respectively are always in the focus of a public opinion. Therefore we got in contact with these local schools, in order to get an impression of the public awareness concerning synthetic biology. Hence we discussed with students about pros and cons of Synthetic Biology, iGEM and our projects as examples for applications. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;This morning was an important experience for the senior school students and also for us. We got some insights about what students think and are concerned about when discussing synthetic biology. Surprinsingly there were not so much concerns about our work as aspected, though the students already gave thought about general aspects of genetical engineering and synthetic biology. With the experience we gained we will improve our human practise work to arouse some interest and hopefully prevented some fear when facing synthetic biology. All in all it was a great morning and as the public discussion is always an important part of iGEM, we encourage other teams to sometimes leave the lab and communiacte with the public.<br><br />
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</p></div><br />
<h3>Yogurt</h3><br />
<div class="tab"><p>Yogurt<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Even the most ordinary biology experiment is fantastic, such as making yogurt, which may be the reason why we iGEMers are so obsessed with it.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;On May19th,at 4:30pm we held a school-wide experiment about making yogurt. About 30 students participated the experiment. Of course, as usual,we introduced the synthetic biology before the experiment. Although most of students probably could not understand the synthetic biology perfectly, they were surprised at the way synthetic biology works in. In reality, everyone who is introduced to the concept of synthetic biology at first is certainly get astonished. Combining engineer and genes is such a miracle, isn’t it? <br> <br />
&nbsp;&nbsp;&nbsp;&nbsp;The activity is held successfully.The participant enjoy biology and show great interest in synthetic biology as well as the IGEM competition.Not only publicize our project but also teach them the advanced scientific thought.We believe they will benefit of it.<br><br><br><br />
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Limu
http://2013hs.igem.org/Notebook/notebook.htm
Notebook/notebook.htm
2013-06-21T19:13:45Z
<p>Limu: </p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 2</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>&nbsp;</td><br />
<td>&nbsp;</td><br />
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<td height="52"><div align="center" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Confirm the project we plan to do. Write the abstract.</div></td><br />
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<tr><br />
<td>&nbsp;</td><br />
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Limu
http://2013hs.igem.org/Team.htm
Team.htm
2013-06-21T19:12:44Z
<p>Limu: </p>
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<img class="full adam" src="https://static.igem.org/mediawiki/2013hs/b/b3/Gaotianlin_a.jpg"><br />
<img class="full ali" src="https://static.igem.org/mediawiki/2013hs/a/ac/Yangfei_a.jpg"><br />
<img class="full anya" src="https://static.igem.org/mediawiki/2013hs/2/2b/Sun_Qinwen_a.jpg"><br />
<img class="full asim" src="https://static.igem.org/mediawiki/2013hs/7/7e/Hongshengnan_a.jpg"><br />
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<img class="full chris" src="https://static.igem.org/mediawiki/2013hs/6/64/Chiyuanyuan_a.jpg"><img src="https://static.igem.org/mediawiki/2013hs/a/ac/Zhangyue_a.jpg" width="196" height="264" class="full cooper" /><img class="full himika" src="https://static.igem.org/mediawiki/2013hs/5/51/Limu_a.jpg"><br />
<img class="full iain" src="https://static.igem.org/mediawiki/2013hs/3/3f/Liuyue_a.jpg"> <br />
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<img class="full anders" src="https://static.igem.org/mediawiki/2013hs/2/28/Xiaokang_a.jpg"><br />
<img class="full doug" src="https://static.igem.org/mediawiki/2013hs/e/e3/Member_01.jpg"><br />
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<div class="desc default"><br />
<h2>Team Profiles</h2><br />
<p>Click the thumbnails below to learn more about each member of the team!</p><br />
<p><a href="https://2012.igem.org/Team:Calgary/Team#next-profile" class="next-profile">Next →</a></p></div><br />
<div class="desc james"><br />
<h2>Huizhi Yu:</h2><br />
<p>As a grade 11 student in shenzhen middle school. I attended the Biology Club immediately after I got into high school. The Biology club teaches me a lot. We had classes every Tuesday afternoon. The first week we observed green vegetables' leave cells and onion epidermal cells with a microscope. The second week we learned about the classification and structure of leaf. Then in the next year I became the president of the Biology Club. We built a ecology tank which the ecological system in the tank provides nutrient for creatures living inside and we do not need to feed them. Also we revised a book on names index of the plants in campus called the Shenzhong Botanic Record and distributed leaf bookmarks made by ourselves in the campus.I appreciate the world's beauty and it acts as my source of passion. I love splendid things from which I can perceive the throb. I pursue knowledge and beauty in ambitious. </p><br />
</div><br />
<div class="desc nick active"><br />
<h2>Liang Xu:</h2><br />
<p>As a student in Shenzhen Middle School,I attend the biology club and the IGEM project.For me,it’s my first time partaking in research,and i have learned volume knowledge about the synthetic biology,gene,engineering and more.I am responsible for the wiki with my partner.In my spare time,i devote myself to music and sports as well as reading.During the time we do the project,i benefit a lot.Not only the lore of biology but also the experience.I believe this will encourage me to get higher aim in my future.</p><br />
<p></p></div><br />
<div class="desc robert"><br />
<h2>Zinan Long:</h2><br />
<p>ZINAN LONG, a 17-years-old student from Shenzhen Middle School,is a member of this team.this is my first year involved with iGEM. I'm responsible for of the team wiki . I really enjoy mostly three things. Basketball, Music, and Computer.I'm excited to be a part of such diverse group of students and can't wait to see what we can come up with!</p><br />
</div><br />
<div class="desc peter"><br />
<h2>Pengxuan Chen :</h2><br />
<p>Personal introduction <br />
Chen Peng Xuan<br />
Leo <br />
I am a member in the team from Shenzhen Middle school who mainly is responsible for the work in laboratory. As a student in the international curriculum of Shenzhen middle school, I learnt a lot from the project of this biological competition. Initially, the biology subject gives me a lot enthusiasm which is one of the most important reasons of why I choose to study abroad. Once I started learning the experiment of molecular biology, the academic atmosphere inside the laboratory did attract me. I really found myself interested in these work, thus I will never be regretful with my choice forever.</p><br />
</div> <br />
<div class="desc adam"><br />
<h2>Tianlin Gao:</h2><br />
<p>Tianlin Gao,<br />
a 17-years-old student from Shenzhen Middle School, Guang Dong Province, China, is a member of this item. In reality, compared with America and Europe, the biology education in China is a little out of date, which cannot arouse students’ interest and passion for biology. However, Gao has been crazy about biology since his junior high school. The mystery of the rule our body obeys, the attractive gene, the magic environment all amazed him. Then, he made a decision that he will devote himself to biology in his rest of life. The iGEM item is his first step on his way to the mystery of biology.</p><br />
</div><br />
<div class="desc ali"><br />
<h2>Fei Yang:</h2><br />
<p>YangFei,was born on 3rd April,1998.I’m fifteen years old now.I like biology very much.And that’s the reason I join this team.I’m in Senior one now but most of my teammates are in Senior two.They know more about this technology.So my responsibility is to learn more about this contest and try my best to help my teammates.I discussed the project with teammates and try to give some useful ideas.After I went to BGI for a week,I learned from the protocol of experiment.So I try to design some process of the experiment and the plan of human practice.I hope to major in biology in university and in work.I believe the 21th century is the century of biology so I expect to learn from the cutting-edge technology in now day.And this is the start. </p><br />
</div><br />
<div class="desc anya"><br />
<h2>Qinwen Sun:</h2><br />
<p> <br />
Hi I’m Sun Qinwen and I’m studying in the Shenzhen Middle School. Synthetic biology is a totally new subject for me! I have been learning it for only half a year, but I am quite interested in it! I feel excited to be part of our team, we went to the lab together and studying, discussing. I am a comparatively young IGEMer in our team, and I learn a lot during this half year.Also, I do the Human Practice in our school, I hope more students would know about IGEM and participate in it. I think the most wonderful thing about IGEM is that it allows us to get closer to science, and even take part in it. And we could create new things! In my free time, I always do physical exercise such as running to keep healthy. Also, I loving eating so I’m learning cooking recently.</p><br />
</div><br />
<div class="desc asim"><br />
<h2>Shengnan Hong :</h2><br />
<p>I am one of the members in the team of Shenzhen middle school who is working mostly in laboratory. As a student in the honor curriculum in Shenzhen middle school, I really appreciate the things I received in there. The school has provided us splendid kinds of chances to find our unique career by our own. I finally select to learn about biology because of my advantage and the original interest in this certain subject. The competition inspired me a lot, and the experiment, despite that it is harder than the normal subject, would eventually be engraved into my mind. </p><br />
</div><br />
<br />
<div class="desc chris"><br />
<h2>Yuanyuan Chi:</h2><br />
<p>Name: Chi Yuanyuan (Winnie)<br />
Class: Class 20 Grade 11 from Shenzhen Middle School<br />
Personal Habit: I often prefer to read books as activity after school, my favorite is fictions and scientific magazines on biology or zoology. Also, drawing some pictures on rough note book and singing a song when walking both bring me a lot of fun.<br />
Responsibilities in the Team: My main responsibility in teams is doing the experiments and accomplishing our program, and I also help settling the data collected during the experiments such as summarizing a complete process of experiment operating.<br />
The Reason of Attending: My main reason of taking part in iGEM is my personal interests in biology---it was my favorite subject since I was in primary school. And I also hope to increase my knowledge on this subject and the experience of activities and competitions in order to prepare better for my future studies.<br />
Future Plan and Hope: I really hope to take part in the jobs related to biology in the future of my life. My greatest hope is becoming a vet in order to tend and heal the animals which were always regarded as close friend to me, and I will also feel pleased and honored to be a researcher on biology and take part in exploring programs on the lives on Earth or become a professor in universities and deliver the knowledge I got.</p><br />
</div><br />
<div class="desc cooper"><br />
<h2>Yue Zhang:</h2><br />
<p>It's really my honor to introduce myself here. In the 16 years since I have been born, I find that how important and interesting that biology is so that I attend the IGEM team of Shenzhen Middle School although I am the student of the Yaohua Experimental School. I really enjoy the time I spend into study biology it also means that the IGEM brings me more time which can make me feel enjoy myself to study in biology. I also like read some books and watch some movies because two things can help me to get some knowledge while I am relaxing myself so that I will think these two things are my hobbits. Biology brings me so many interest that I think there is no subject is as interesting and important as biology. The things we do in these months are not only for the IGEM but also for ourselves to have a really good time to let ourselves totally addict into biology in a more professional way without any disturb. Because of these reasons, I think it is a right decision for me to decide to attend the IGEM and it makes me feel I know more about biology and myself.</p><br />
</div><br />
<div class="desc himika"><br />
<h2>Mu Li:</h2><br />
<p> Turor Li Mu is our main guider on arranging our route and working progress, he meets and discusses with us every week on the team meeting, and gives us advices on our working direction and time based on the data we provided. On the other hand, he also help us solve the fundamental working problem such as contacting the travel agency and asking for leaving the school when we should be in laboratory. Lu Mu is an enlightened tutor who usually respects our own choice and tells us to decide our rode by ourselves.</p><br />
</div><br />
<div class="desc iain"><br />
<h2>Yue Liu: </h2><br />
<p>Tutor Liu Yue is mainly responsible for the biology club in Shenzhen Middle School, many activities held by biology club such as vivarium manufacture, the “plant journal” of Shenzhen Middle School compiling and biological theme blackboard newspaper were pushed forward by her. Also, she pays great attention on us which used to be the central unions of biology club, she often asks us about our progress and gives us many opportunities to advertise our works, who is a tutor with great interest and enthusiasm on biology.</p><br />
</div> <br />
<div class="desc anders"><br />
<h2> Kang Xiao: </h2><br />
<p>Tutor Xiao Kang is our experiment guider, he is a doctoral student of Hong Kong University of Science and Technology with great experience and professional skills in biological experiments. He was often being with us during our days in laboratory, watched and guided us on the concrete steps and attentions when doing the experiments. Xiao Kang is a young talent with friendly, easy-going personality and great patience, he will always explain every details and problems to us without any impatience or annoy.</p><br />
</div><br />
<div class="desc doug"><br />
<h2>Kang Kang:</h2><br />
<p> Tutor Kang Kang is the main theory guider of our team; he gave us the lectures of synthetic biology which was the fundamental knowledge for us to begin our progress, and also, he discussed our idea with us and finally helped us decide the idea we used in attending the competition and provided us the place we can complete our experiment---the BGI graduate school. Kang Kang is a wise tutor with outright personality who often directly criticized our ideas on its defects so we can improve them in time and accurately.</p><br />
</div><br />
<div class="desc lisa_g"><br />
<h2>Dr. Lisa Gieg</h2><br />
<p>I have been doing research in the field of environmental microbiology for about 20 years. I hold a Ph.D. from the University of Alberta, and worked for a dozen years as a PDF and Senior Researcher at the University of<br />
Oklahoma before coming to the University of Calgary as an Assistant Professor in Biological Sciences. My research focuses on petroleum metabolism by microbes in the context of oil spill clean-up, microbial enhanced energy recovery, and in oil sands tailings ponds remediation. This is my second year being involved with iGEM. It is a pleasure to work with dynamic, smart, and talented students and colleagues who are exploring the use of synthetic biology to solve real world problems.</p><br />
</div><br />
<div class="desc mayi"><br />
<h2>Dr. Mayi Arcellana-Panlilio</h2><br />
<p>I am an adjunct associate professor in the Department of Biochemistry &amp; Molecular Biology. I have been involved with teaching/mentoring in the Bachelor of Health Sciences program since 2003, primarily to develop and deliver the Honours Cell &amp; Molecular Biology course, a core requirement in the curriculum. I have taught every offering of that course and received numerous accolades from the Faculty and the students, most recently winning the Teaching Excellence Award for 2010-2011 from the University of Calgary Students Union. This is my second year as an iGEM Instructor and my interest in the program springs from my belief in the value of inquiry as a means to getting students engaged in the pursuit of knowledge and developing habits of becoming lifelong learners.</p><br />
</div><br />
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<td width="20">&nbsp;</td><br />
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<td width="21">&nbsp;</td><br />
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<td width="21">&nbsp;</td><br />
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<td width="21">&nbsp;</td><br />
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<td width="20">&nbsp;</td><br />
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Limu
http://2013hs.igem.org/Project.htm
Project.htm
2013-06-21T19:12:09Z
<p>Limu: </p>
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<td><p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nitrate (NO3-) and nitrite (NO2-) are chemical roots of nitric acid (HNO3) and nitrous acid (HNO2) respectively. They exist in the aquatic environment, the living organisms and artificial products, as pollutants, which can cause food poisoning, cancers or even death. It's important to detect and eliminate such chemicals with the efficient methods. Currently, several detection methods have already been in use but all are not entirely harmless. For example, granular Cd grains are recommended for the NO3- determination for fruits and vegetables because they are easy to make and recover, and can be used repeatedly. However, Cd cannot be recycled easily, and may cause additional pollution. So the goal of our project is to provide an efficient and a more environment friendly method of detecting nitrates/nitrites and we strive to design biosensors to avoid the disadvantages of traditional methods.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Specific details of the pollution of the Nitrites and Nitrates:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nitrate (NO3-) and nitrite (NO2-) are Chemical roots of nitric acid (HNO3) and nitrous acid (HNO2). They exist in nature, especially in the gaseous water, surface water and groundwater, as well as plants and animals and food within, as environmental pollutants. <br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;There exist a lot of pollution sources of nitrate and nitrite in the environment, such as: <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. Artificial fertilizers: ammonium nitrate, calcium nitrate, potassium nitrate, sodium nitrate and urea;<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Sewage, garbage and human and animal feces: according to tests, 1 liters of domestic sewage in the natural degradation process can produce 110 mg of nitrate; 1 kg of garbage and fecal compost under natural conditions can produce 492 mg of nitrate leaching decomposition;<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. Large amounts of ammonia which food, fuel or oil refining factories emit in waste: after nature biological, chemical conversion cause the formation of nitrate into the environment; <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;4. Cars, trains, ships, aircraft, boilers, civil furnace combustion of petroleum fuels, coal, natural gas: can produce large amounts of nitrogen oxides. Average burning one ton of coal, of 1 kiloliter oil and 10,000 cubic meters of natural gas can produce nitrogen dioxide gas, respectively 9.13 and 63 kg. These nitrogen dioxide gas can be formed after precipitation leaching nitrate descend to the ground and waters ; <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;5. Food preservation and preservation: nitrate and nitrite are widely used in corrosion protection and preservation of meat and fish, so that the meat is red and aroma. Adding sodium nitrate per kilogram of meat in food (usually sodium nitrite) 5 mg or less, within a certain time will percept a good flesh color ; added 20 mg or more, and may exhibit the needed stability in the commercial color; added 50 mg of a special smell.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;These chemical fertilizers in irrigation, sewage, garbage and fecal, industrial nitrogenous waste, fuel combustion, emissions containing nitrogen gas and other pollutions, after precipitation leaching and decomposition in natural conditions, will form nitrate into the river, the lake and into the ground, resulting in nitrate pollution of surface water and groundwater. Infiltration such as sewage, sewage irrigation and unrestrained use of fertilizers sharp increase in groundwater nitrate content can be from a few mg / l to 400 mg / l or more (the national life of the nitrate content of drinking water health standards less than 88.6 mg / l to nitrogen of less than 20 mg / l); indiscriminate use of fertilizers, irrigation, nitrate pollution of water irrigation cause crops absorb large amounts of nitrates, such as over-fertilization spinach production per kilogram dry weight containing nitrite 3600 mg of salt. Moreover, pickled sauerkraut, vegetables after the lon&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;g-distance transport and long-term storage, as well as overnight cooked vegetables are all unhealthy. Because not only a large number of nitrate content increased, but also in the role of nitrate-reducing bacteria, nitrates are reduced to nitrites.<br><br />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Large amounts of nitrate and nitrite contain in drinking water, vegetables, grain, fish, meat, sauerkraut, overnight cooking, allow direct poisoning by human consumption. Nitrate in the human body can also be reduced to nitrite. Nitrite reacts in human blood; cause the formation of methemoglobin, so that the blood loss of oxygen functionality makes oxygen poisoning. The symptoms caused by it such as dizziness on light, palpitations, vomiting, lips cyanosis, severe cases, unconsciousness, convulsions, shortness of breath, can be life threatening if not rescued in time. Moreover, nitrite may react with secondary amines and from nitrosamines in the environment both in and out human bodies, which will become carcinogenic, teratogenic and mutagenic substances if reaches a certain dose, this will totally do great harm to human health.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In order to prevent the hazard of nitrate and nitrite, in addition to achieve scientific and rational application of fertilizer, prohibit the use of sewage irrigation ,implement the manure treatment of sewage and garbage and other environmental protection measures to protect surface and ground water sources from nitrate and nitrite nitrate pollution, human should also keep a minimum to eat pickled, smoked, wax system of meat, canned food, stains sauerkraut, and salted vegetables; does not buy foods that were kept too long, lethargic or tertian; finish the food right after buying them; does not eat overnight cooked vegetables or keep tertian boiled vegetables; and remember not to confuse the industrial nitrite with normal salt for eating.<br><br />
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<td><p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Introduction:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Our team is going to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia coli (E.coli) chassis. To introduce the whole process of our project, we split our works into 3 parts.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Design</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Firstly, we aim to build a part containing the nitrates/nitrites sensing promoter and the most visible and observable reporter. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This can also be called a development from previous work of Edinburgh 2009. Their aim was to enable E.coli to report the existence of nitrates/nitrites with green fluorescent protein (GFP) and the PyeaR promoter (the sensor of the nitrates/nitrites they created), but we found several drawbacks of their work. Firstly, the GFP reporter within the bacteria is not efficient enough to respond to the inducer so only dim green fluorescence can be detected. Also, this biosensor cannot be recycled for repeated usage because it does not have an efficient way to turn off the reporting process after the detection. Thus, our team aims to improve the biosensor by taking addition of a more remarkable and observable reporter. From the parts registry, we eventually find a possible reporter, the CrtEBI, that, when synthesized, will lead the E. coli to release a specific pigment, lycopene, as the visible color to determine the existence of nitrates/nitrites. Simultaneously, we will incorporate another reporter, the banana odor enzyme (ATF1) generator, which enables our biosensor to produce an aroma of bananas with the existence of nitrates/nitrites. Therefore, our biosensor containing dual reporters may ensure a high accuracy in the detection of nitrates and nitrites.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Secondly, our team planned to build another part that is functional to eliminate the effect given by part 1. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The first step that we took in this plan was finding a reporter in the registry that can cause the degradation of the pigment. More specifically, the proteins produced by this appropriate reporter are useful to degrade the pigment, so that after easily adding certain promoter to control it, this part 2 that we were willing to build could restore the bacteria to the original state. But the fact was crucial. We could not find this reporter at any sources that we were capable to reach. Based on this result, we started step 2, another solution that might enabled us to finish our design. According to the Web sources of the Peking University 2010 (the creator of the CrtEBI), we learned that the pigment, lycopene would be naturally degrade by bacteria in an appropriate acidic environment or under high temperature. Thus we aimed to find any reporter or protein that could produce heat or acid. Although there were some of the proteins in this range, we could not choose them because of the indeterminacy of their experiment, and we failed again as a result. But in the final step, we eventually thought of a more effective and simple method of this part. We planned to find a part that can reduce the expression of the proteins. According to the description of the CrtEBI, which produces enzymes that convert colorless farnesyl pyrophosphate to red lycopene, the only thing we need to do in this part is to enervate the enzymes. Although the pigment still would not disappear, the bacteria would stop producing the pigment and thus it is convenient for us to eliminate the remaining pigment by changing PH or temperature because of the part 2 that we made. <br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Thirdly, our team aimed to enhance our biobricks by designing a color gradient system which can mark the various concentrations of the nitrates/nitrites.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The overall design of the color gradient system is to repeatedly add different color (nestification) to reveal the different molarities of the nitrites and nitrates. Based on the work by Peking University, we found several different colors with the same function as the CrtEBI. Such as the BBa_K274002,BBa_K274001.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To give a simple example, If we linked the PyeaR promoter , the color reporter 1 (we assume that it is red) and a protein reporter ( we assume that it is TetR reporter) together as the first part, then linked the color reporter 2 ( assumed that it is green) and a TetR negative promoter together as the second part, we could probably observe that the color change from green to grey (as the red color was added) when the concentration of nitrites and nitrates was increasing. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To finish this part 3, the most important work for us is to create an appropriate model as measurement of the input, the nitrites and nitrates, and the output, the variable colors. <br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>MORE DETAILS</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Promoter:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We choose the the PyeaR promoter because it is the only suitable one as the essence of our project obviously. It can be found easily on the parts registry: BBa_K216005<br />
Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions. It was built based on the work of Edinburgh 2009.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<b>Reporter:</b><br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The CrtEBI which can cause the bacteria to produce the red pigment, lycopene is suitable for our team to replace the conventional part, GFP. It can be also found in parts registry: BBa_K274100<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Inside the reporter, together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a colored signal output, e.g. for biosensors.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This design has been created with a list of other different colorful designs. For example, a related Composite Biobrick is Part BBa_K274200 (CrtEBIY with rbs), which "goes on" one step after lycopene, converting red lycopene to orange beta-carotene pigment.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The banana odor enzyme (ATF1) generator was a standard reporter given by MIT 2006. Many other projects has used this design as a remarkable reporter. It can be found in the parts registry: BBa_J45199<br><br />
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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2013hs/7/7f/Part_01.jpg"></p><br />
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<td><p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Outcome If we can finish the entire plan hopefully, we are willing to have such these result.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;By inserting the first system that we built into the bacteria, we will receive a sensor which, after absorb the nitrites or nitrates, can efficiently produce the remarkable symbols such as pigments and banana odor.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;By inserting the second system into the bacteria, we can let the bacteria be capable to clean all the products it produced previously, so that the target of our experiment can be reused.<br><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;By inserting the final system of our project, we can create a sensor which will produce a color gradient after approaching to the nitrites and nitrates. From this goal we are willing to reach, we can observe the visual change of the bacteria easily, and simultaneously receive a beautiful and cheerful ending of our whole project in the IGEM competition.<br><br />
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Limu
http://2013hs.igem.org/Team:Shenzhen_SZMS
Team:Shenzhen SZMS
2013-06-21T19:11:33Z
<p>Limu: </p>
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<td width="20">&nbsp;</td><br />
<td width="472"><span class="STYLE6">N</span><span class="STYLE1">itrate (NO3-) and nitrite (NO2-) are chemical roots of nitric acid (HNO3) and nitrous acid (HNO2) respectively. They exist in the aquatic environment, the living organisms and artificial products, as pollutants, which can cause food poisoning, cancers or even death. It's important to detect and eliminate such chemicals with the efficient methods. Currently, several detection methods have already been in use but all are not entirely harmless. For example, granular Cd grains are recommended for the NO3- determination for fruits and vegetables because they are easy to make and recover, and can be used repeatedly. However, Cd cannot be recycled easily, and may cause additional pollution. So the goal of our project is to provide an efficient and a more environment friendly method of detecting nitrates/nitrites and we strive to design biosensors to avoid the disadvantages of traditional methods. </span> <br />
<p class="STYLE1">Our team is going to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia</p></td><br />
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<td width="84%"><img src="https://static.igem.org/mediawiki/2013hs/b/be/Project_02.jpg" width="426" height="47" /></td><br />
<td width="16%"><img src="https://static.igem.org/mediawiki/2013hs/4/4b/Project_03.jpg" width="84" height="47" /></td><br />
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<div id="epicbox"><br />
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<div align="center" style="color:#CF900F;background:969696;height:35px;width:126px "><br />
<b>OVERVIEW</b><br />
</div><br />
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<br />
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<div align="center" style="color:#CF900F;background:969696;height:35px;width:126px "><br />
<b>OUTREACH</b><br />
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</a><br />
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<div align="center" style="color:#CF900F;background:969696;height:35px;width:126px "><br />
<b>PICTURE</b><br />
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<h2>Human Practices</h2><br />
<p>In order to make people know more about synthetic biology and IGEM,we use extracurricular to publicity our competition project.We also organize some activity to let people know it............</p><br />
</div><br />
<div class="orangebox" style="display: none;"><br />
<h2>Human Practices</h2><br />
<p>In order to make people know more about synthetic biology and IGEM,we use extracurricular to publicity our competition project.We also organize some activity to let people know it............</p><br />
</div><br />
<div class="obox1" style="display: none;"><br />
<h2>Initiative</h2><br />
<p>We took the initiative with the oil sands industry and established a dialogue between industry experts, academics, and government representatives. Through these talks a roadmap for the use of synthetic biology in the oil sands was established, focusing on biosensing and bioremediation.</p><br />
</div><br />
<div class="obox2" style="display: none;"><br />
<h2>Interviews</h2><br />
<p>As an undergraduate team, we spoke with experts in various fields, including the oil industry, tailings pond management, biotechnology, law, and politics to gather various opinions on our project. How useful is synthetic biology in a tailings pond environment? What design considerations should we include in our project to improve security? What legal policies must we consider before implementing our project?</p><br />
</div><br />
<div class="obox3" style="display: none;"><br />
<h2>Design Considerations</h2><br />
<p>Since safety is the driving force behind our project, we need to ensure the physical design of our biosensor and bioreactor would contain and manage the bacteria to minimize the possibility of their escape. We needed to build devices that would ensure the safety of both the user and the outside environment during their use. Click here to learn more!</p><br />
</div><br />
<div class="obox4" style="display: none;"><br />
<h2>Killswitch</h2><br />
<p>Both FRED and OSCAR are designed to operate within enclosed environments. However, since safety is our highest priority, we decided to design and implement a killswitch in both FRED and OSCAR as an extra layer of security. The killswitch aims to destroy the genome using two powerful non-specific nucleases in the unlikely event that bacteria escape into the environment. Click here to learn more!</p><br />
</div><br />
<div class="obox5" style="display: none;"><br />
<h2>Safety</h2><br />
<p>Click here for our safety page! Here we detail all the safety procedures, certifications, and approvals we have from our University to allow us to work this summer.</p><br />
</div><br />
<div class="obox6" style="display: none;"><br />
<h2>Community Outreach</h2><br />
<p>iGEM Calgary partnered with a number of different associations to engage the general public about science and synthetic biology. Click here to see what we've done this summer!</p><br />
</div><br />
<div class="greenbox" style="display: none;"><br />
<h2>TEAM</h2><br />
<p> It contains the team member and our directive teacher.Also our school’s introduction and the method of contacting us..................</p><br />
</div><br />
<div class="gbox1" style="display: none;"><br />
<h2>Detecting</h2><br />
<p>FRED is our star detective, working around the clock to detect toxins roaming freely in tailings pond water. FRED is known for being a little unorthodox in his methods namely by using a measurable electrochemical signal rather than colored or fluorescent reporters. Using his transposon library FRED gets clues about the genetic elements that will activate in the presence of toxins. </p><br />
</div><br />
<div class="gbox2" style="display: none;"><br />
<h2>Reporting</h2><br />
<p>Once FRED finds the toxins he wastes no time exposing them. Building upon last year's single output electrochemical system FRED now uses the new triple output system to report the toxins. This novel approach to electrochemical reporting means that FRED is as good as he is fast. </p><br />
</div><br />
<div class="gbox3" style="display: none;"><br />
<h2>Modelling</h2><br />
<p>Aside from being a stellar detective FRED also dabbles in the art of mathematics and modelling. These skills are used to model the behavior of the system. The results from the modelling helped guide the wet lab experiments which in turn gave new data to refine the model.</p><br />
</div><br />
<div class="gbox4" style="display: none;"><br />
<h2>Prototyping</h2><br />
<p>FRED gets a lot of information so he stays on top of things with the help of his handy dandy physical device to test the sample and software to interpret the raw data. The device has been <b>prototyped</b> and has an accompanying software platform that works with FRED to detect the toxins. </p><br />
</div><br />
<div class="bluebox" style="display: none;"><br />
<h2>OTHER</h2><br />
<p>There are the other part of project,including supporter and safety questions.........</p><br />
</div><br />
<div class="bbox1" style="display: none;"><br />
<h2>Overview</h2><br />
<p>Our team is going to develop novel modular biosensors that enable cost-effective and on-site detection of nitrates/nitrites by using an Escherichia coli (E.coli) chassis. To introduce the whole process of our project, we split our works into 3 parts......</p><br />
</div><br />
<div class="bbox2" style="display: none;"><br />
<h2>Outreach</h2><br />
<p><img src="https://static.igem.org/mediawiki/2013hs/0/09/Outreach_pic.jpg" /></p><br />
</div><br />
<div class="bbox3" style="display: none;"><br />
<h2>PICTURE</h2><br />
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<p>OSCAR needed a home, so we developed an enclosed bioreactor system where toxins can be converted into hydrocarbons for output. Click here to see how we designed the bioreactor!</p><br />
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<h2>Upgrading</h2><br />
<p>Nitrogen and sulfur heteroatoms can produce nasty airborne pollutants when burned, cause acid rain and acid deposition, and can damage valuable catalysis mechanisms involved in fuel processing and emissions control. OSCAR can remove these atoms trapped in the rings. Click here to see how!</p><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1" valign="top"><p><span class="STYLE7">F</span>irstly, let us show our great appreciation and respect to our four tutors: Li Mu, Kang Kang, Xiao Kang and Liu Yue, without their help on school and daily affairs solving, theory and experiment supporting and the unremittingly attention and encouragement, it can be impossible for us to achieve the fruit now, their every lecture for us, every note writing on our experiment notebook and every meeting held with us together make up our nowadays success.<br /><br />
Also, we should thank the BGI graduate school which bountifully provided us the proper laboratory, advanced equipment and abundant material to complete our experiments and researches. Although how brilliant or impeccable our ideas are, we can’t make them come true without BGI’s resource offering. Not to mention the favorable condition of board and lodging they provided for us during the days we stayed overnight.<br /><br />
And at last, thank for our almamater, Shenzhen Middle School’s supporting and approving on our research, if they didn’t allow us to drop several national classes in completing our experiments, our achievements will also be debarred in papers and our mind instead of the shining light on petri dishes which stands for success.</p><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1"><h1>&nbsp;</h1><br />
<p><strong>1. Would any of your project ideas raise safety issues in terms of:</strong><br /><br />
<strong>RESEARCHER SAFETY</strong><br /><br />
We have not worked with any materials that raise risk to researchers' safety or health. We only worked with experiments based on commonly used E. coli and baking yeast strains. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely.<br /><br />
Sometime our researchers have to face regular bio-safety challenges in a molecular microbiology lab, such as staining DNA agarose gel with EB, each member of our team is careful to use gloves, goggles, and lab coats in order to protect us .There is also a container for us to pour culture fluid and culture medium full of cells in, and thus it will be treated properly afterwards.<br /><br />
Before the real experiment each member of our team took 2 weeks to receive training. Any researchers conducting experiment were instructed about the right method both by printed protocols and in the lab. After the training, we passed an on-line test about the lab safety, standard experiment procedures and lab waste treatment. During any experiments, necessary individual protections such as gloves and white robes were strictly required. Also, the public safety of the lab is guaranteed by the strict registering protocols on the operation of any apparatus. In order to make sure all the requirements of safety were carried out well by the researchers and to get prepared for any emergency, our lab set up 24hr*7 camera system in the rooms.<br /><br />
<strong>PUBLIC SAFETY</strong><br /><br />
As we said, what we used are non-pathogenic E. coli strains. We will use the experimental plasmid containing antibiotics. So one of the security risks is that antibiotic resistance gene may be transferred to other pathogenic strains and cause the public danger.<br /><br />
To avoid it happening, we gather all the culture fluid and culture medium with cells for unified treatment and not let cells get out of our lab through anyway. <br /><br />
<strong>ENVIRONMENTAL SAFETY</strong><br /><br />
We take much care of the environmental safety. We make sure no media containing engineered cells go into the sewer before sterilization, and all utensil contacted with the engineered bacteria will be sterilized. Toxic and hazardous chemicals are collected and treated properly according to the safety rules of our lab.<br /><br />
<br /><br />
<strong>2. Do any of the new BioBrick parts (or devices) that you made this year raise safety issues?</strong><br /><br />
NO. We don’t think that our new devices will raise safety issues. Most of our BioBricks have been used by former iGEM projects and proved to be innocuous too.<br /><br />
<br /><br />
<strong>3. Is there a local biosafety group, committee, or review board at your institution? </strong><br /><br />
<strong>If yes, what does your local biosafety group think about your project? </strong><br /><br />
<strong>If no, which specific biosafety rules or guidelines do you have to consider in your country? </strong><br /><br />
<strong>BIOSAFETY PROVISIONS</strong><br /><br />
Our experiment is designed and carried out following the standards of national biosafety office, therefore no laws or regulations regarding the biosafety issue are violated.<br /><br />
Because our school (Shenzhen Middle School) has only a brand new laboratory which is established in 2011, so far, we do not have a biosafety committee in our institute. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely. Many teachers and assistants from biology and chemistry department of our school care our safety and have checked the laboratory we used and the processes we have done. And they think the condition we were working is safe and reliable.<br /><br />
<br /><br />
<strong>1.Do you have any other ideas how to deal with safety issues that could be</strong><strong>useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong><br /><br />
<br /><br />
Yes. We suggest the registry of standard biological parts should have a dedicated safety page of every part to ensure that users would not raise any safety issue when using them. And these data should be presented when we are searching for a certain part and found its instructions. By this act, we can at least prevent the safety issues lurking in the shadows before they could appear.<strong> </strong></p></td><br />
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Human.htm
2013-06-21T19:04:30Z
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<h3>Publicity</h3><br />
<div class="tab"><p><b>human practise</b><br /><br />
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&nbsp;&nbsp;&nbsp;&nbsp;The final plan of our team to propagate our project and the synthetic biology is to disseminate those to the citizens directly. We decided to summarize the main content of our project and print it out, in order to make it accessible for all the citizens to see in the most convenient way. Then we planned to bring all those products to public, the city enter so that we would disseminate them to as much citizens as we can.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;We finished print the booklets before going out to the public. The content of the booklet is simpler than that from our wikis. But it is easier for the citizens to understand. <br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;After discussing, we eventually chose to go to a book city (a huge book shop), a hospital and a estate with many high-tech companies in it. The main groups of people we decide to talk with are students, parents and old men who would probably take more attention to our scientific and biological project.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;This is the visual evidence of our activities of the human practice.<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/7/74/Human_01.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/2/28/Human_02.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/e/e8/Human_03.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/f/f3/Human_04.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/6/61/Human_05.jpg" width="292" height="170"><br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;During our activities, we accepted an interview of a reporter who was interested to put our experience to the newspaper of next day. We are very happy to talk about the details of our project to the reporter and finally successful to explain the main motivation of our project and to illustrate a summary of the synthetic biology and the IGEM competition.<br /><br />
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&nbsp;&nbsp;&nbsp;&nbsp;<b>Our project was reported by the local newspaper.</b><br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/d/d9/Human_06.jpg" width="292" height="170"><br><br />
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Translation:<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;We were caught by some students of Shenzhen Middle School.According to their words,they invent a e-coli which can detect the Nitrite.They also publicized their manual with the detailed project.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;‘Nitrite is everywhere. It can harm people’s health through the way we can’t be aware of ’ Said by Shenzhen Middle School’s students.In order to prevent it.They transform an e-coli. They put genes that can be aware of the Nitrite and link a special alarm after the gene. When it meet the Nitrite,it can touch off the alarm so that, people can detect the Nitrite straightly! Citizens said.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;When we ask them about the experiment, they say ‘It’s very difficult.’In the laboratory, they can’t finish the experiment with their senior high’s knowledge.Not only learn the knowledge,but also practice their skill.All their result now is combined of tears and fortune.<br /><br />
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</p></div><br />
<h3>Presentantion</h3><br />
<div class="tab wushi"><p>Lectures on Synthetic Biology <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;School visit is part of our project that could directly inform the high school students about synthetic biology. We visited Yaohua High School, Sixth High School and Nanshan School affiliated with China National Institute for Educational Research(SZyangxiao). And we gave a presentation on the Synthetic Biology and iGEM. Looking down on the faces of these students, we knew that some of whom may end up doing biological research a few years later.<br><br />
Yaohua High School<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;We introduced the conception of Synthetic Biology and showed some slices about the harmful effect of nitrate and nitrite. Then we presented iGEM and our project to them. We also answered some questions asked by the curious senior school students. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Synthetic biology is still in an early developing stage in China and it is believed to bring new opportunities to biotechnology and to solve critical problems and scares that we might face now and in the future, like food and fuel crisis. However, similar to the situation in western countries, synthetic biology is still in an emerging field in China that lacks clear definition and safety laws. The lack of a well-accepted definition and safety concerns do not seem to stop the Chinese government and scientists from pursuing synthetic biology. In recent years, high standard labs and institutions have been set up featuring mainly on research of synthetic biology. Funds from Chinese government on synthetic biology increases significantly. There are several universities that are playing the leading role in the field of synthetic biology in China, while more and more universities and colleges are joining in. A good example to show the Chinese students’ curiosity on synthetic biology might be the increasing number of universities that took part in the iGEM competition through the past five years.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Our lectures and discussion sessions were open to anyone who wanted to join. Because the class was for people of all experience levels, our lecture started as an explanation of the most important biological mechanisms. Building on the basics, then we introduced synthetic biology both within the context of iGEM and as it is understood in some of the most significant synbio laboratories. When we felt like the basics of synthetic biology had been covered, we were keen to explore its public perception as well as the possibilities of its future. We involved discussion groups on topics from the ethics of synthetic biology to its public image to the problem of intellectual property. <br><br />
The sixth high school<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;To popularize the concept and spirit of synthetic biology,SZMS iGEM team held a lecture on June 11th, which was mainly oriented to the grade 10 students in The Sixtn High School, as well as all the other students who were interested in synthetic biology.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;The preparation of the lecture started 3 weeks prior to the lecture. Posters were elaborately designed and posted up around the campus. We also bulletined the news through E-mail and phone calls, to ensure every potential audience might be interested in it and took part in.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;On the afternoon of June 11th, we were flattered the moment walking into meeting place, for the room was almost filled up. Never had we thought that the lecture would attract so many audiences. The audiences, most of which were grade 10 students, responded warmly to the splendid lectures given by the three speakers. Chen Pengxuan first gave an overall introduction of the history of synthetic biology. And then he went on with several detailed programs and potential applications on theory of synthetic biology. The audiences were shocked by the marvelous and multiple work done by the engineered bacteria in a popular and easy-to-understand language. The talks took ninety minutes in all, while the question and answer session lasted for another half an hour. His wonderful speech in his unique humorous style won him warm applause from the audiences in the end.<br><br />
SZyangxiao<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;In order to promote synthetic biology and iGEM, we decided to give a presentation to a senior school, SZyangxiao. During the presentation, we give them a brief introduction of the basic knowledge,research strategy , application and industrialization of synthetic biology. Furthermore, we also introduced several interesting iGEM projects. In the end of the presentation, we introduced our iGEM project this year in a plain way. With the help of the presentation and their basic knowledge on the synthetic biology, they have a very clear idea about what iGEM is like as well as our project when we finished.<br> <br />
&nbsp;&nbsp;&nbsp;&nbsp;As synthetic biology is going to be the next big thing in biology, its researches are always raising public concerns respectively are always in the focus of a public opinion. Therefore we got in contact with these local schools, in order to get an impression of the public awareness concerning synthetic biology. Hence we discussed with students about pros and cons of Synthetic Biology, iGEM and our projects as examples for applications. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;This morning was an important experience for the senior school students and also for us. We got some insights about what students think and are concerned about when discussing synthetic biology. Surprinsingly there were not so much concerns about our work as aspected, though the students already gave thought about general aspects of genetical engineering and synthetic biology. With the experience we gained we will improve our human practise work to arouse some interest and hopefully prevented some fear when facing synthetic biology. All in all it was a great morning and as the public discussion is always an important part of iGEM, we encourage other teams to sometimes leave the lab and communiacte with the public.<br><br />
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</p></div><br />
<h3>Yogurt</h3><br />
<div class="tab"><p>Yogurt<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Even the most ordinary biology experiment is fantastic, such as making yogurt, which may be the reason why we iGEMers are so obsessed with it.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;On May19th,at 4:30pm we held a school-wide experiment about making yogurt. About 30 students participated the experiment. Of course, as usual,we introduced the synthetic biology before the experiment. Although most of students probably could not understand the synthetic biology perfectly, they were surprised at the way synthetic biology works in. In reality, everyone who is introduced to the concept of synthetic biology at first is certainly get astonished. Combining engineer and genes is such a miracle, isn’t it? <br> <br />
&nbsp;&nbsp;&nbsp;&nbsp;The activity is held successfully.The participant enjoy biology and show great interest in synthetic biology as well as the IGEM competition.Not only publicize our project but also teach them the advanced scientific thought.We believe they will benefit of it.<br><br><br><br />
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Limu
http://2013hs.igem.org/Wiki/notebook_0530.htm
Wiki/notebook 0530.htm
2013-06-21T19:03:16Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 30</td><br />
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<p style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Wiki accomplished</p><br />
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Limu
http://2013hs.igem.org/Wiki/notebook_0528.htm
Wiki/notebook 0528.htm
2013-06-21T19:01:04Z
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 28</td><br />
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<p align="center" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Achieve the final aim.Complete the Project.</p><br />
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Limu
http://2013hs.igem.org/Wiki/notebook_0519.htm
Wiki/notebook 0519.htm
2013-06-21T18:59:35Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 19</td><br />
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<p align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">The e-coli detect the nitrate successfully.However it is light-colored.<br /><br />
We determined to improve it.</p><br />
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Limu
http://2013hs.igem.org/Wiki/notebook_0507.htm
Wiki/notebook 0507.htm
2013-06-21T18:58:22Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">May 7</td><br />
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<p align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Ensure our uniform and the Team Logo.</p><br />
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Limu
http://2013hs.igem.org/Wiki/notebook_0503.htm
Wiki/notebook 0503.htm
2013-06-21T18:55:43Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<p align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Begin to make wiki.<br /><br />
We read a lot about how to make website and ask for professional information.It really make us confused because it’s so difficult to make a great wiki!Fortunately,we accomplish it........</p><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0409.htm
Notebook/notebook 0409.htm
2013-06-21T18:43:31Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="81%" class="STYLE7">&nbsp;</td><br />
<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 9</td><br />
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<td>&nbsp;</td><br />
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<table width="761" border="0" cellspacing="0" cellpadding="0" bgcolor="#F8F8F8"><br />
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<td height="52"><div align="center" class="STYLE6"><br />
<p style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Cut the promoter and other parts.Link them.</p><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0405.htm
Notebook/notebook 0405.htm
2013-06-21T18:32:06Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 5</td><br />
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<td width="89%"><div align="center" class="STYLE6"><br />
<div align="left" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Find the parts we need in case.Devise how to do it.</div><br />
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Limu
http://2013hs.igem.org/Notebook/jsp/home.htm
Notebook/jsp/home.htm
2013-06-21T18:16:28Z
<p>Limu: Blanked the page</p>
<hr />
<div></div>
Limu
http://2013hs.igem.org/Notebook/jsp/home.htm
Notebook/jsp/home.htm
2013-06-21T18:13:57Z
<p>Limu: </p>
<hr />
<div><div id="greenbox"><br />
<a href="https://2013hs.igem.org/Team:Shenzhen_SZMS"><br />
<div class="imgbox"></div>
Limu
http://2013hs.igem.org/Notebook/jsp/home.htm
Notebook/jsp/home.htm
2013-06-21T18:12:55Z
<p>Limu: Created page with "<a href="https://2013hs.igem.org/Team:Shenzhen_SZMS">"</p>
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<div><a href="https://2013hs.igem.org/Team:Shenzhen_SZMS"></div>
Limu
http://2013hs.igem.org/Notebook/jsp/other_02.htm
Notebook/jsp/other 02.htm
2013-06-21T18:09:19Z
<p>Limu: </p>
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<td width="94%" style="font-size:24px;color:#00904b; ">Acknowledgments</td><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1" valign="top"><p><span class="STYLE7">F</span>irstly, let us show our great appreciation and respect to our four tutors: Li Mu, Kang Kang, Xiao Kang and Liu Yue, without their help on school and daily affairs solving, theory and experiment supporting and the unremittingly attention and encouragement, it can be impossible for us to achieve the fruit now, their every lecture for us, every note writing on our experiment notebook and every meeting held with us together make up our nowadays success.<br /><br />
Also, we should thank the BGI graduate school which bountifully provided us the proper laboratory, advanced equipment and abundant material to complete our experiments and researches. Although how brilliant or impeccable our ideas are, we can’t make them come true without BGI’s resource offering. Not to mention the favorable condition of board and lodging they provided for us during the days we stayed overnight.<br /><br />
And at last, thank for our almamater, Shenzhen Middle School’s supporting and approving on our research, if they didn’t allow us to drop several national classes in completing our experiments, our achievements will also be debarred in papers and our mind instead of the shining light on petri dishes which stands for success.</p><br />
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Limu
http://2013hs.igem.org/Notebook/jsp/other_02.htm
Notebook/jsp/other 02.htm
2013-06-21T18:08:07Z
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1" valign="top"><p><span class="STYLE7">F</span>irstly, let us show our great appreciation and respect to our four tutors: Li Mu, Kang Kang, Xiao Kang and Liu Yue, without their help on school and daily affairs solving, theory and experiment supporting and the unremittingly attention and encouragement, it can be impossible for us to achieve the fruit now, their every lecture for us, every note writing on our experiment notebook and every meeting held with us together make up our nowadays success.<br /><br />
Also, we should thank the BGI graduate school which bountifully provided us the proper laboratory, advanced equipment and abundant material to complete our experiments and researches. Although how brilliant or impeccable our ideas are, we can’t make them come true without BGI’s resource offering. Not to mention the favorable condition of board and lodging they provided for us during the days we stayed overnight.<br /><br />
And at last, thank for our almamater, Shenzhen Middle School’s supporting and approving on our research, if they didn’t allow us to drop several national classes in completing our experiments, our achievements will also be debarred in papers and our mind instead of the shining light on petri dishes which stands for success.</p><br />
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Limu
http://2013hs.igem.org/Notebook/jsp/other_01.htm
Notebook/jsp/other 01.htm
2013-06-21T18:05:51Z
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1"><h1>&nbsp;</h1><br />
<p><strong>1. Would any of your project ideas raise safety issues in terms of:</strong><br /><br />
<strong>RESEARCHER SAFETY</strong><br /><br />
We have not worked with any materials that raise risk to researchers' safety or health. We only worked with experiments based on commonly used E. coli and baking yeast strains. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely.<br /><br />
Sometime our researchers have to face regular bio-safety challenges in a molecular microbiology lab, such as staining DNA agarose gel with EB, each member of our team is careful to use gloves, goggles, and lab coats in order to protect us .There is also a container for us to pour culture fluid and culture medium full of cells in, and thus it will be treated properly afterwards.<br /><br />
Before the real experiment each member of our team took 2 weeks to receive training. Any researchers conducting experiment were instructed about the right method both by printed protocols and in the lab. After the training, we passed an on-line test about the lab safety, standard experiment procedures and lab waste treatment. During any experiments, necessary individual protections such as gloves and white robes were strictly required. Also, the public safety of the lab is guaranteed by the strict registering protocols on the operation of any apparatus. In order to make sure all the requirements of safety were carried out well by the researchers and to get prepared for any emergency, our lab set up 24hr*7 camera system in the rooms.<br /><br />
<strong>PUBLIC SAFETY</strong><br /><br />
As we said, what we used are non-pathogenic E. coli strains. We will use the experimental plasmid containing antibiotics. So one of the security risks is that antibiotic resistance gene may be transferred to other pathogenic strains and cause the public danger.<br /><br />
To avoid it happening, we gather all the culture fluid and culture medium with cells for unified treatment and not let cells get out of our lab through anyway. <br /><br />
<strong>ENVIRONMENTAL SAFETY</strong><br /><br />
We take much care of the environmental safety. We make sure no media containing engineered cells go into the sewer before sterilization, and all utensil contacted with the engineered bacteria will be sterilized. Toxic and hazardous chemicals are collected and treated properly according to the safety rules of our lab.<br /><br />
<br /><br />
<strong>2. Do any of the new BioBrick parts (or devices) that you made this year raise safety issues?</strong><br /><br />
NO. We don’t think that our new devices will raise safety issues. Most of our BioBricks have been used by former iGEM projects and proved to be innocuous too.<br /><br />
<br /><br />
<strong>3. Is there a local biosafety group, committee, or review board at your institution? </strong><br /><br />
<strong>If yes, what does your local biosafety group think about your project? </strong><br /><br />
<strong>If no, which specific biosafety rules or guidelines do you have to consider in your country? </strong><br /><br />
<strong>BIOSAFETY PROVISIONS</strong><br /><br />
Our experiment is designed and carried out following the standards of national biosafety office, therefore no laws or regulations regarding the biosafety issue are violated.<br /><br />
Because our school (Shenzhen Middle School) has only a brand new laboratory which is established in 2011, so far, we do not have a biosafety committee in our institute. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely. Many teachers and assistants from biology and chemistry department of our school care our safety and have checked the laboratory we used and the processes we have done. And they think the condition we were working is safe and reliable.<br /><br />
<br /><br />
<strong>1.Do you have any other ideas how to deal with safety issues that could be</strong><strong>useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong><br /><br />
<br /><br />
Yes. We suggest the registry of standard biological parts should have a dedicated safety page of every part to ensure that users would not raise any safety issue when using them. And these data should be presented when we are searching for a certain part and found its instructions. By this act, we can at least prevent the safety issues lurking in the shadows before they could appear.<strong> </strong></p></td><br />
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Limu
http://2013hs.igem.org/Notebook/jsp/other.htm
Notebook/jsp/other.htm
2013-06-21T18:04:45Z
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<td width="92%"><a href="other_01.htm" target="main"><font color="#000000"><span class="STYLE6">Safety Questions</span></font></a></td><br />
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<td width="94%" style="font-size:24px;color:#00904b; ">iGEM Safety Questions</td><br />
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<td width="86%" bgcolor="#F8F8F8" class="STYLE1"><h1>&nbsp;</h1><br />
<p><strong>1. Would any of your project ideas raise safety issues in terms of:</strong><br /><br />
<strong>RESEARCHER SAFETY</strong><br /><br />
We have not worked with any materials that raise risk to researchers' safety or health. We only worked with experiments based on commonly used E. coli and baking yeast strains. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely.<br /><br />
Sometime our researchers have to face regular bio-safety challenges in a molecular microbiology lab, such as staining DNA agarose gel with EB, each member of our team is careful to use gloves, goggles, and lab coats in order to protect us .There is also a container for us to pour culture fluid and culture medium full of cells in, and thus it will be treated properly afterwards.<br /><br />
Before the real experiment each member of our team took 2 weeks to receive training. Any researchers conducting experiment were instructed about the right method both by printed protocols and in the lab. After the training, we passed an on-line test about the lab safety, standard experiment procedures and lab waste treatment. During any experiments, necessary individual protections such as gloves and white robes were strictly required. Also, the public safety of the lab is guaranteed by the strict registering protocols on the operation of any apparatus. In order to make sure all the requirements of safety were carried out well by the researchers and to get prepared for any emergency, our lab set up 24hr*7 camera system in the rooms.<br /><br />
<strong>PUBLIC SAFETY</strong><br /><br />
As we said, what we used are non-pathogenic E. coli strains. We will use the experimental plasmid containing antibiotics. So one of the security risks is that antibiotic resistance gene may be transferred to other pathogenic strains and cause the public danger.<br /><br />
To avoid it happening, we gather all the culture fluid and culture medium with cells for unified treatment and not let cells get out of our lab through anyway. <br /><br />
<strong>ENVIRONMENTAL SAFETY</strong><br /><br />
We take much care of the environmental safety. We make sure no media containing engineered cells go into the sewer before sterilization, and all utensil contacted with the engineered bacteria will be sterilized. Toxic and hazardous chemicals are collected and treated properly according to the safety rules of our lab.<br /><br />
<br /><br />
<strong>2. Do any of the new BioBrick parts (or devices) that you made this year raise safety issues?</strong><br /><br />
NO. We don’t think that our new devices will raise safety issues. Most of our BioBricks have been used by former iGEM projects and proved to be innocuous too.<br /><br />
<br /><br />
<strong>3. Is there a local biosafety group, committee, or review board at your institution? </strong><br /><br />
<strong>If yes, what does your local biosafety group think about your project? </strong><br /><br />
<strong>If no, which specific biosafety rules or guidelines do you have to consider in your country? </strong><br /><br />
<strong>BIOSAFETY PROVISIONS</strong><br /><br />
Our experiment is designed and carried out following the standards of national biosafety office, therefore no laws or regulations regarding the biosafety issue are violated.<br /><br />
Because our school (Shenzhen Middle School) has only a brand new laboratory which is established in 2011, so far, we do not have a biosafety committee in our institute. We performed our experiment mostly in another institute BGI nearby, their lab fulfill the requirement of Lab Biosafety level 2, which enable us to perform bacterial (E.coli) experiment safely. Many teachers and assistants from biology and chemistry department of our school care our safety and have checked the laboratory we used and the processes we have done. And they think the condition we were working is safe and reliable.<br /><br />
<br /><br />
<strong>1.Do you have any other ideas how to deal with safety issues that could be</strong><strong>useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong><br /><br />
<br /><br />
Yes. We suggest the registry of standard biological parts should have a dedicated safety page of every part to ensure that users would not raise any safety issue when using them. And these data should be presented when we are searching for a certain part and found its instructions. By this act, we can at least prevent the safety issues lurking in the shadows before they could appear.<strong> </strong></p></td><br />
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Limu
http://2013hs.igem.org/Notebook/human.htm
Notebook/human.htm
2013-06-21T18:00:59Z
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<h3>Publicity</h3><br />
<div class="tab"><p><b>human practise</b><br /><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;The final plan of our team to propagate our project and the synthetic biology is to disseminate those to the citizens directly. We decided to summarize the main content of our project and print it out, in order to make it accessible for all the citizens to see in the most convenient way. Then we planned to bring all those products to public, the city enter so that we would disseminate them to as much citizens as we can.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;We finished print the booklets before going out to the public. The content of the booklet is simpler than that from our wikis. But it is easier for the citizens to understand. <br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;After discussing, we eventually chose to go to a book city (a huge book shop), a hospital and a estate with many high-tech companies in it. The main groups of people we decide to talk with are students, parents and old men who would probably take more attention to our scientific and biological project.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;This is the visual evidence of our activities of the human practice.<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/7/74/Human_01.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/2/28/Human_02.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/e/e8/Human_03.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/f/f3/Human_04.jpg" width="292" height="170"><br />
<img src="https://static.igem.org/mediawiki/2013hs/6/61/Human_05.jpg" width="292" height="170"><br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;During our activities, we accepted an interview of a reporter who was interested to put our experience to the newspaper of next day. We are very happy to talk about the details of our project to the reporter and finally successful to explain the main motivation of our project and to illustrate a summary of the synthetic biology and the IGEM competition.<br /><br />
<br />
&nbsp;&nbsp;&nbsp;&nbsp;<b>Our project was reported by the local newspaper.</b><br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/d/d9/Human_06.jpg" width="292" height="170"><br><br />
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Translation:<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;We were caught by some students of Shenzhen Middle School.According to their words,they invent a e-coli which can detect the Nitrite.They also publicized their manual with the detailed project.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;‘Nitrite is everywhere. It can harm people’s health through the way we can’t be aware of ’ Said by Shenzhen Middle School’s students.In order to prevent it.They transform an e-coli. They put genes that can be aware of the Nitrite and link a special alarm after the gene. When it meet the Nitrite,it can touch off the alarm so that, people can detect the Nitrite straightly! Citizens said.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;When we ask them about the experiment, they say ‘It’s very difficult.’In the laboratory, they can’t finish the experiment with their senior high’s knowledge.Not only learn the knowledge,but also practice their skill.All their result now is combined of tears and fortune.<br /><br />
<br />
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<h3>Presentantion</h3><br />
<div class="tab wushi"><p>Lectures on Synthetic Biology <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;School visit is part of our project that could directly inform the high school students about synthetic biology. We visited Yaohua High School, Sixth High School and Nanshan School affiliated with China National Institute for Educational Research(SZyangxiao). And we gave a presentation on the Synthetic Biology and iGEM. Looking down on the faces of these students, we knew that some of whom may end up doing biological research a few years later.<br><br />
Yaohua High School<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;We introduced the conception of Synthetic Biology and showed some slices about the harmful effect of nitrate and nitrite. Then we presented iGEM and our project to them. We also answered some questions asked by the curious senior school students. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Synthetic biology is still in an early developing stage in China and it is believed to bring new opportunities to biotechnology and to solve critical problems and scares that we might face now and in the future, like food and fuel crisis. However, similar to the situation in western countries, synthetic biology is still in an emerging field in China that lacks clear definition and safety laws. The lack of a well-accepted definition and safety concerns do not seem to stop the Chinese government and scientists from pursuing synthetic biology. In recent years, high standard labs and institutions have been set up featuring mainly on research of synthetic biology. Funds from Chinese government on synthetic biology increases significantly. There are several universities that are playing the leading role in the field of synthetic biology in China, while more and more universities and colleges are joining in. A good example to show the Chinese students’ curiosity on synthetic biology might be the increasing number of universities that took part in the iGEM competition through the past five years.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Our lectures and discussion sessions were open to anyone who wanted to join. Because the class was for people of all experience levels, our lecture started as an explanation of the most important biological mechanisms. Building on the basics, then we introduced synthetic biology both within the context of iGEM and as it is understood in some of the most significant synbio laboratories. When we felt like the basics of synthetic biology had been covered, we were keen to explore its public perception as well as the possibilities of its future. We involved discussion groups on topics from the ethics of synthetic biology to its public image to the problem of intellectual property. <br><br />
The sixth high school<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;To popularize the concept and spirit of synthetic biology,SZMS iGEM team held a lecture on June 11th, which was mainly oriented to the grade 10 students in The Sixtn High School, as well as all the other students who were interested in synthetic biology.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;The preparation of the lecture started 3 weeks prior to the lecture. Posters were elaborately designed and posted up around the campus. We also bulletined the news through E-mail and phone calls, to ensure every potential audience might be interested in it and took part in.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;On the afternoon of June 11th, we were flattered the moment walking into meeting place, for the room was almost filled up. Never had we thought that the lecture would attract so many audiences. The audiences, most of which were grade 10 students, responded warmly to the splendid lectures given by the three speakers. Chen Pengxuan first gave an overall introduction of the history of synthetic biology. And then he went on with several detailed programs and potential applications on theory of synthetic biology. The audiences were shocked by the marvelous and multiple work done by the engineered bacteria in a popular and easy-to-understand language. The talks took ninety minutes in all, while the question and answer session lasted for another half an hour. His wonderful speech in his unique humorous style won him warm applause from the audiences in the end.<br><br />
SZyangxiao<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;In order to promote synthetic biology and iGEM, we decided to give a presentation to a senior school, SZyangxiao. During the presentation, we give them a brief introduction of the basic knowledge,research strategy , application and industrialization of synthetic biology. Furthermore, we also introduced several interesting iGEM projects. In the end of the presentation, we introduced our iGEM project this year in a plain way. With the help of the presentation and their basic knowledge on the synthetic biology, they have a very clear idea about what iGEM is like as well as our project when we finished.<br> <br />
&nbsp;&nbsp;&nbsp;&nbsp;As synthetic biology is going to be the next big thing in biology, its researches are always raising public concerns respectively are always in the focus of a public opinion. Therefore we got in contact with these local schools, in order to get an impression of the public awareness concerning synthetic biology. Hence we discussed with students about pros and cons of Synthetic Biology, iGEM and our projects as examples for applications. <br><br />
&nbsp;&nbsp;&nbsp;&nbsp;This morning was an important experience for the senior school students and also for us. We got some insights about what students think and are concerned about when discussing synthetic biology. Surprinsingly there were not so much concerns about our work as aspected, though the students already gave thought about general aspects of genetical engineering and synthetic biology. With the experience we gained we will improve our human practise work to arouse some interest and hopefully prevented some fear when facing synthetic biology. All in all it was a great morning and as the public discussion is always an important part of iGEM, we encourage other teams to sometimes leave the lab and communiacte with the public.<br><br />
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</p></div><br />
<h3>Yogurt</h3><br />
<div class="tab"><p>Yogurt<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;Even the most ordinary biology experiment is fantastic, such as making yogurt, which may be the reason why we iGEMers are so obsessed with it.<br><br />
&nbsp;&nbsp;&nbsp;&nbsp;On May19th,at 4:30pm we held a school-wide experiment about making yogurt. About 30 students participated the experiment. Of course, as usual,we introduced the synthetic biology before the experiment. Although most of students probably could not understand the synthetic biology perfectly, they were surprised at the way synthetic biology works in. In reality, everyone who is introduced to the concept of synthetic biology at first is certainly get astonished. Combining engineer and genes is such a miracle, isn’t it? <br> <br />
&nbsp;&nbsp;&nbsp;&nbsp;The activity is held successfully.The participant enjoy biology and show great interest in synthetic biology as well as the IGEM competition.Not only publicize our project but also teach them the advanced scientific thought.We believe they will benefit of it.<br><br><br><br />
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Limu
http://2013hs.igem.org/Notebook/notebook_0402.htm
Notebook/notebook 0402.htm
2013-06-21T16:42:51Z
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<td height="84" background="https://static.igem.org/mediawiki/2013hs/1/17/Notebook_02.jpg"><table width="100%" height="64" border="0" cellpadding="0" cellspacing="0"><br />
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<td width="81%" class="STYLE7">&nbsp;</td><br />
<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 2</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>&nbsp;</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table></td><br />
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<table width="761" border="0" cellspacing="0" cellpadding="0" bgcolor="#F8F8F8"><br />
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<td width="91%" valign="top" class="STYLE1"><table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td height="52"><div align="center" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Confirm the project we plan to do. Write the abstract.</div></td><br />
</tr><br />
</table><br />
<table width="69%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td>&nbsp;</td><br />
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Limu
http://2013hs.igem.org/Notebook/notebook.htm
Notebook/notebook.htm
2013-06-21T16:41:58Z
<p>Limu: Created page with "<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=gb2312"> <title>无标题文档</title> </head> <body> <table width="916" border="0" align="center" cel..."</p>
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<tr><br />
<td width="81%" class="STYLE7">&nbsp;</td><br />
<td width="19%" style="font-family: Arial, Helvetica, sans-serif; font-size: 24px;color: #00904b;">April 2</td><br />
<br />
<br />
</tr><br />
<tr><br />
<td>&nbsp;</td><br />
<td>&nbsp;</td><br />
</tr><br />
</table></td><br />
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</table> <br />
<table width="761" border="0" cellspacing="0" cellpadding="0" bgcolor="#F8F8F8"><br />
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<td width="4%" height="331">&nbsp;</td><br />
<td width="91%" valign="top" class="STYLE1"><table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td height="52"><div align="center" style="font-family: Arial, Helvetica, sans-serif; font-size: 18px; ">Confirm the project we plan to do. Write the abstract.</div></td><br />
</tr><br />
</table><br />
<table width="69%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td>&nbsp;</td><br />
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<br /><br />
<table width="69%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td><img src="https://static.igem.org/mediawiki/2013hs/1/16/Notebook_03.jpg" width="747" height="58" /></td><br />
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Limu
http://2013hs.igem.org/Team_02.htm
Team 02.htm
2013-06-21T15:59:06Z
<p>Limu: </p>
<hr />
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<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team.htm">Member</a></span></td><br />
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<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
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<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
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<td width="4%">&nbsp;</td><br />
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<td width="92%"><span class="STYLE6"><a href="team_03.htm">Contact us</a></span></td><br />
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<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_02.htm">Outtakes</a></a></span></td><br />
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<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
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<td width="64%" bgcolor="#F7F7F7">&nbsp;</td><br />
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<br /><br />
Our human practice!<br />
<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/1/16/Out_take2.jpg" /><br /><br />
Our experiment note book! How assiduous we are~<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/8/84/Out_take3.jpg" /><br /><br />
Our hero^^<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/5/5d/Out_take4.jpg" /><br /><br />
How beautiful!......at least on me.<br />
<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/4/40/Out_take6.jpg" /><br /><br />
After a long time boring meeting. A inspiration of our logo!<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/5/5a/Out_take7.jpg" /><br /><br />
Two lovely girls!^^<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/3/3f/Out_take8.jpg" /><br /><br />
Yummy! <br />
<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/b/b7/Out_take9.jpg" /><br /><br />
Cute loli~~<br /><br />
At last all of us!<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/1/11/Out_take10.jpg" /><br /><br />
<br /><br />
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</td><br />
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<td width="245"><img src="https://static.igem.org/mediawiki/2013hs/c/c7/Buttom_01.jpg" width="245" height="166" /></td><br />
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</html></div>
Limu
http://2013hs.igem.org/Team_02.htm
Team 02.htm
2013-06-21T15:54:45Z
<p>Limu: </p>
<hr />
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<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
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<td width="92%"><span class="STYLE6"><a href="team_03.htm">Contact us</a></span></td><br />
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<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_02.htm">Outtakes</a></a></span></td><br />
</tr><br />
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</table></td><br />
<td width="13"></td><br />
<td width="673" valign="top"><table width="100%" height="312" border="0" cellpadding="0" cellspacing="0"><br />
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<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
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</tr><br />
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<td height="293" bgcolor="#F7F7F7">&nbsp;</td><br />
<td colspan="2" valign="top" bgcolor="#F7F7F7"><img src="images/out_take.jpg" /><br />
<br /><br />
Our human practice!<br />
<br /><br />
<img src="images/out_take2.jpg" /><br /><br />
Our experiment note book! How assiduous we are~<br /><br />
<img src="images/out_take3.jpg" /><br /><br />
Our hero^^<br /><br />
<img src="images/out_take4.jpg" /><br /><br />
How beautiful!......at least on me.<br />
<img src="images/out_take5.jpg" /><br /><br />
How beautiful!...........at least on me.<br />
<br /><br />
<img src="images/out_take6.jpg" /><br /><br />
After a long time boring meeting. A inspiration of our logo!<br /><br />
<img src="images/out_take7.jpg" /><br /><br />
Two sexy girls!^^<br /><br />
<img src="images/out_take8.jpg" /><br /><br />
Yummy! <br />
<br /><br />
<img src="images/out_take9.jpg" /><br /><br />
Cute loli~~<br /><br />
At last all of us!<br /><br />
<img src="images/out_take10.jpg" /><br /><br />
<br /><br />
<br />
</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
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<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
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<td>&nbsp;</td><br />
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</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="245"><img src="https://static.igem.org/mediawiki/2013hs/c/c7/Buttom_01.jpg" width="245" height="166" /></td><br />
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<td><img src="https://static.igem.org/mediawiki/2013hs/4/4e/Buttom_03.jpg" /></td><br />
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</table></td><br />
<td width="330"><img src="https://static.igem.org/mediawiki/2013hs/3/3e/Buttom_04.jpg" /></td><br />
</tr><br />
</table><br />
</body><br />
</html></div>
Limu
http://2013hs.igem.org/Team_02.htm
Team 02.htm
2013-06-21T15:53:07Z
<p>Limu: Created page with "<html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <title>Outtakes</title> <style type="text/css"> <!-- body..."</p>
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#apDiv1 {<br />
position:absolute;<br />
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text-decoration: none;<br />
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text-decoration: none;<br />
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--><br />
</style><br />
</head><br />
<br />
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<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td width="116"><a href="home.htm"><img src="https://static.igem.org/mediawiki/2013hs/a/a0/Title_home.jpg" width="116" height="76" / border="0"></a></td><br />
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</tr><br />
</table><br />
<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td width="230" height="576" valign="top"><table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td width="510" height="51" valign="middle" background="images/team_02.jpg"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
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<td width="4%">&nbsp;</td><br />
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<td width="92%"><span class="STYLE6"><a href="team.htm">Member</a></span></td><br />
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</table></td><br />
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<tr><br />
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<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
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<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_03.htm">Contact us</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_02.htm">Outtakes</a></a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table></td><br />
<td width="13"></td><br />
<td width="673" valign="top"><table width="100%" height="312" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
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<td width="64%" bgcolor="#F7F7F7">&nbsp;</td><br />
</tr><br />
<tr><br />
<td height="293" bgcolor="#F7F7F7">&nbsp;</td><br />
<td colspan="2" valign="top" bgcolor="#F7F7F7"><img src="images/out_take.jpg" /><br />
<br /><br />
Our human practice!<br />
<br /><br />
<img src="images/out_take2.jpg" /><br /><br />
Our experiment note book! How assiduous we are~<br /><br />
<img src="images/out_take3.jpg" /><br /><br />
Our hero^^<br /><br />
<img src="images/out_take4.jpg" /><br /><br />
How beautiful!......at least on me.<br />
<img src="images/out_take5.jpg" /><br /><br />
How beautiful!...........at least on me.<br />
<br /><br />
<img src="images/out_take6.jpg" /><br /><br />
After a long time boring meeting. A inspiration of our logo!<br /><br />
<img src="images/out_take7.jpg" /><br /><br />
Two sexy girls!^^<br /><br />
<img src="images/out_take8.jpg" /><br /><br />
Yummy! <br />
<br /><br />
<img src="images/out_take9.jpg" /><br /><br />
Cute loli~~<br /><br />
At last all of us!<br /><br />
<img src="images/out_take10.jpg" /><br /><br />
<br /><br />
<br />
</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
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<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
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<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="245"><img src="images/buttom_01.jpg" width="245" height="166" /></td><br />
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<tr><br />
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</tr><br />
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</table><br />
</body><br />
</html></div>
Limu
http://2013hs.igem.org/Team_03.htm
Team 03.htm
2013-06-21T15:48:54Z
<p>Limu: Created page with "<html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <title>Contact us</title> <style type="text/css"> <!-- bo..."</p>
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#apDiv1 {<br />
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</style><br />
</head><br />
<br />
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<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td width="116"><a href="home.htm"><img src="https://static.igem.org/mediawiki/2013hs/a/a0/Title_home.jpg" width="116" height="76" / border="0"></a></td><br />
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<td width="254">&nbsp;</td><br />
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<td width="4%">&nbsp;</td><br />
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<td width="92%"><span class="STYLE6"><a href="team.htm">Member</a></span></td><br />
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</table></td><br />
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<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
</tr><br />
</table></td><br />
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<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_03.htm">Contact us</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
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<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_02.htm">Outtakes</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table></td><br />
<td width="13"></td><br />
<td width="673" valign="top"><table width="100%" height="312" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
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</tr><br />
<tr><br />
<td height="293" bgcolor="#F7F7F7">&nbsp;</td><br />
<td colspan="2" valign="top" bgcolor="#F7F7F7"><p class="STYLE1"><span class="STYLE9">c</span>ontact us<br />
<br />
we are shenzhen middle school iGEM team!<br /><br />
If you want to contact us, you can log in our school website www.shenzhong.net<br /><br />
or you can send email to lim746@126.com.<br /><br />
</p></td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="245"><img src="https://static.igem.org/mediawiki/2013hs/c/c7/Buttom_01.jpg" width="245" height="166" /></td><br />
<td background="https://static.igem.org/mediawiki/2013hs/5/52/Buttom_02.jpg"><table width="10%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td><img src="https://static.igem.org/mediawiki/2013hs/4/4e/Buttom_03.jpg" /></td><br />
</tr><br />
</table></td><br />
<td width="330"><img src="https://static.igem.org/mediawiki/2013hs/3/3e/Buttom_04.jpg" /></td><br />
</tr><br />
</table><br />
</body><br />
</html></div>
Limu
http://2013hs.igem.org/Team_01.htm
Team 01.htm
2013-06-21T15:42:00Z
<p>Limu: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>School</title><br />
<style type="text/css"><br />
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</style><br />
</head><br />
<br />
<body><br />
<br />
<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="116"><a href="home.htm"><img src="https://static.igem.org/mediawiki/2013hs/a/a0/Title_home.jpg" width="116" height="76" / border="0"></a></td><br />
<td width="144"><a href="project.htm"><img src="https://static.igem.org/mediawiki/2013hs/c/c2/Title-project.jpg" width="144" height="76" / border="0"></a></td><br />
<td width="112"><a href="team.htm"><img src="https://static.igem.org/mediawiki/2013hs/e/ed/Title_team.jpg" width="112" height="76" / border="0"></a></td><br />
<td width="173"><a href="notebook/notebook.htm"><img src="https://static.igem.org/mediawiki/2013hs/2/25/Title-notebook.jpg" width="173" height="76" / border="0"></a></td><br />
<td width="254"><a href="human.htm"><img src="https://static.igem.org/mediawiki/2013hs/4/41/Title_human-practice.jpg" width="254" height="76" / border="0"></a></td><br />
<td width="117"><a href="jsp/other.htm"><img src="https://static.igem.org/mediawiki/2013hs/7/7d/Title-other.jpg" width="116" height="76" / border="0"></a></td><br />
</tr><br />
</table><br />
<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="116" height="36">&nbsp;</td><br />
<td width="144">&nbsp;</td><br />
<td width="112">&nbsp;</td><br />
<td width="173">&nbsp;</td><br />
<td width="254">&nbsp;</td><br />
<td width="117">&nbsp;</td><br />
</tr><br />
</table><br />
<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="576" valign="top"><table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" background="images/team_02.jpg"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team.htm">Member</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_03.htm">Contact us</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_02.htm">Outtakes</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table></td><br />
<td width="13"></td><br />
<td width="673" valign="top"><table width="100%" height="312" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
<td width="32%" bgcolor="#F7F7F7">&nbsp;</td><br />
<td width="64%" bgcolor="#F7F7F7">&nbsp;</td><br />
</tr><br />
<tr><br />
<td height="293" bgcolor="#F7F7F7">&nbsp;</td><br />
<td colspan="2" valign="top" bgcolor="#F7F7F7"><p class="STYLE1"><span class="STYLE9">S</span>chool Introduction---Shenzhen Middle School: <br /><br />
&nbsp;Shenzhen Middle School was founded in 1947, and was affirmed as the only provincial key middle school in June 1983, the fast improving and well effective school in 1986, the sample school of the national new curriculum standard in 2004 and one of the first group of schools which pass Cantonese national exemplary high school junior assess in June 2006. Shenzhen Middle School passed the assess of national exemplary school, and precisely raised the mission of “building the technicality high school and fostering innovation-type talents” and the working definition of “building the outstanding academic Chinese high school during the international period”.<br /><br />
&nbsp;The high school club of Shenzhen Middle School was consisted of the two campuses of East and West, it covers the area of 9.8 square kilometers with a collection of 1150 thousand books, and preliminary structures the cyber fundamental facilities and essential service supporting platform with the goal of achieving the digitization, webified and informatization of environment, resource and activity.<br /><br />
&nbsp;At the moment, the high school club has 60 academic classes with students reaching the number of 2872. And those students of Shenzhen Middle School all gets brilliant academic achievement the grade of college entrance examination has the advanced advantage among the whole city and even whole province, the percent of undergraduate course and emphasis school are always keeping still above 90% and 60% since middle of the nineties, and over 200 students successfully entered Beijing and Qinghua Universities which are the best universities in China in the average number of 30 annually.<br /><br />
Shenzhen Middle School also has abundant fruit on international field: it has won nine gold metals of international Olympic competition and over 200 students of it entered the universities in the top 50 of USA.<br /><br />
Furtherly, in order to promote the comprehensive development, foster the wholesome personality and cultivate the aesthetic sentiment of students, besides providing superior studying conditions, Shenzhen Middle School also offers its students affluent after-class life platforms. In Shenzhen Middle School, we can find student organization such as school Youth League Committee, student union, committee of student congress and the senior group; the activities such as soccer games, basketball match, sports festival, culture and art festival, cultural lecture, Shenzhong Forum, subject week and expert lecture; more than fifty student clubs such as chorus, wind band, dance troupe, volunteer team, drama club, astronomy club, parkour club, simulative U.N. and National Middle School Student Panda Protecting Organization. All of these activities and organizations are the important platform for students to attend social practice and develop personalities freely.<br /><br />
Shenzhen Middle School was named as National Artistic Advanced School, National Aesthetic Curriculum Reform Experimental School and Cantonese Artistic Education Exemplary School in 2010 by Ministry of Education. The famous clubs of chorus, wind band and dance troupe has repeatedly won golden awards in international aesthetic competitions and won fourteen national first awards and one second award in only three periods of national artistic exhibition. And noticeably, the chorus of Shenzhen Middle School has won the gold award of Olympic Chorusing Competition for continuously three years.<br /><br />
<img src="https://static.igem.org/mediawiki/2013hs/8/82/School.jpg" /><br />
<br />
</p></td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="245"><img src="https://static.igem.org/mediawiki/2013hs/c/c7/Buttom_01.jpg" width="245" height="166" /></td><br />
<td background="https://static.igem.org/mediawiki/2013hs/5/52/Buttom_02.jpg"><table width="10%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td><img src="https://static.igem.org/mediawiki/2013hs/4/4e/Buttom_03.jpg" /></td><br />
</tr><br />
</table></td><br />
<td width="330"><img src="https://static.igem.org/mediawiki/2013hs/3/3e/Buttom_04.jpg" /></td><br />
</tr><br />
</table><br />
</body><br />
</html></div>
Limu
http://2013hs.igem.org/Team_01.htm
Team 01.htm
2013-06-21T15:40:11Z
<p>Limu: Created page with "<html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <title>School</title> <style type="text/css"> <!-- body {..."</p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<title>School</title><br />
<style type="text/css"><br />
<!--<br />
body {<br />
margin-left: 0px;<br />
margin-top: 0px;<br />
margin-right: 0px;<br />
margin-bottom: 0px;<br />
}<br />
.STYLE6 {<br />
font-size: 18px;<br />
font-family: Arial, Helvetica, sans-serif;<br />
}<br />
#apDiv1 {<br />
position:absolute;<br />
left:139px;<br />
top:38px;<br />
width:142px;<br />
height:158px;<br />
z-index:1;<br />
}<br />
.STYLE7 { font-family: Arial, Helvetica, sans-serif;<br />
font-size: 24px;<br />
color: #00904b;<br />
}<br />
.STYLE9 {font-family: Arial, Helvetica, sans-serif; font-size: 24px; color: #000000; }<br />
.STYLE1 { font-family: Arial, Helvetica, sans-serif;<br />
font-size: 14px;<br />
line-height: 24px;<br />
}<br />
.STYLE2 {font-size: 24px}<br />
a:link {<br />
text-decoration: none;<br />
color: #000000;<br />
}<br />
a:visited {<br />
text-decoration: none;<br />
color: #000000;<br />
}<br />
a:hover {<br />
text-decoration: none;<br />
}<br />
a:active {<br />
text-decoration: none;<br />
}<br />
--><br />
</style><br />
</head><br />
<br />
<body><br />
<br />
<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="116"><a href="home.htm"><img src="https://static.igem.org/mediawiki/2013hs/a/a0/Title_home.jpg" width="116" height="76" / border="0"></a></td><br />
<td width="144"><a href="project.htm"><img src="https://static.igem.org/mediawiki/2013hs/c/c2/Title-project.jpg" width="144" height="76" / border="0"></a></td><br />
<td width="112"><a href="team.htm"><img src="https://static.igem.org/mediawiki/2013hs/e/ed/Title_team.jpg" width="112" height="76" / border="0"></a></td><br />
<td width="173"><a href="notebook/notebook.htm"><img src="https://static.igem.org/mediawiki/2013hs/2/25/Title-notebook.jpg" width="173" height="76" / border="0"></a></td><br />
<td width="254"><a href="human.htm"><img src="https://static.igem.org/mediawiki/2013hs/4/41/Title_human-practice.jpg" width="254" height="76" / border="0"></a></td><br />
<td width="117"><a href="jsp/other.htm"><img src="https://static.igem.org/mediawiki/2013hs/7/7d/Title-other.jpg" width="116" height="76" / border="0"></a></td><br />
</tr><br />
</table><br />
<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="116" height="36">&nbsp;</td><br />
<td width="144">&nbsp;</td><br />
<td width="112">&nbsp;</td><br />
<td width="173">&nbsp;</td><br />
<td width="254">&nbsp;</td><br />
<td width="117">&nbsp;</td><br />
</tr><br />
</table><br />
<table width="916" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="576" valign="top"><table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" background="images/team_02.jpg"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team.htm">Member</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_01.htm">About the high school</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_03.htm">Contact us</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="230" height="1"></td><br />
</tr><br />
</table><br />
<table width="230" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="510" height="51" valign="middle" bgcolor="#e5e5e5"><table width="100%" height="43" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%">&nbsp;</td><br />
<td width="4%">&nbsp;</td><br />
<td width="92%"><span class="STYLE6"><a href="team_02.htm">Outtakes</a></span></td><br />
</tr><br />
</table></td><br />
</tr><br />
</table></td><br />
<td width="13"></td><br />
<td width="673" valign="top"><table width="100%" height="312" border="0" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td width="4%" bgcolor="#F7F7F7">&nbsp;</td><br />
<td width="32%" bgcolor="#F7F7F7">&nbsp;</td><br />
<td width="64%" bgcolor="#F7F7F7">&nbsp;</td><br />
</tr><br />
<tr><br />
<td height="293" bgcolor="#F7F7F7">&nbsp;</td><br />
<td colspan="2" valign="top" bgcolor="#F7F7F7"><p class="STYLE1"><span class="STYLE9">S</span>chool Introduction---Shenzhen Middle School: <br /><br />
&nbsp;Shenzhen Middle School was founded in 1947, and was affirmed as the only provincial key middle school in June 1983, the fast improving and well effective school in 1986, the sample school of the national new curriculum standard in 2004 and one of the first group of schools which pass Cantonese national exemplary high school junior assess in June 2006. Shenzhen Middle School passed the assess of national exemplary school, and precisely raised the mission of “building the technicality high school and fostering innovation-type talents” and the working definition of “building the outstanding academic Chinese high school during the international period”.<br /><br />
&nbsp;The high school club of Shenzhen Middle School was consisted of the two campuses of East and West, it covers the area of 9.8 square kilometers with a collection of 1150 thousand books, and preliminary structures the cyber fundamental facilities and essential service supporting platform with the goal of achieving the digitization, webified and informatization of environment, resource and activity.<br /><br />
&nbsp;At the moment, the high school club has 60 academic classes with students reaching the number of 2872. And those students of Shenzhen Middle School all gets brilliant academic achievement the grade of college entrance examination has the advanced advantage among the whole city and even whole province, the percent of undergraduate course and emphasis school are always keeping still above 90% and 60% since middle of the nineties, and over 200 students successfully entered Beijing and Qinghua Universities which are the best universities in China in the average number of 30 annually.<br /><br />
Shenzhen Middle School also has abundant fruit on international field: it has won nine gold metals of international Olympic competition and over 200 students of it entered the universities in the top 50 of USA.<br /><br />
Furtherly, in order to promote the comprehensive development, foster the wholesome personality and cultivate the aesthetic sentiment of students, besides providing superior studying conditions, Shenzhen Middle School also offers its students affluent after-class life platforms. In Shenzhen Middle School, we can find student organization such as school Youth League Committee, student union, committee of student congress and the senior group; the activities such as soccer games, basketball match, sports festival, culture and art festival, cultural lecture, Shenzhong Forum, subject week and expert lecture; more than fifty student clubs such as chorus, wind band, dance troupe, volunteer team, drama club, astronomy club, parkour club, simulative U.N. and National Middle School Student Panda Protecting Organization. All of these activities and organizations are the important platform for students to attend social practice and develop personalities freely.<br /><br />
Shenzhen Middle School was named as National Artistic Advanced School, National Aesthetic Curriculum Reform Experimental School and Cantonese Artistic Education Exemplary School in 2010 by Ministry of Education. The famous clubs of chorus, wind band and dance troupe has repeatedly won golden awards in international aesthetic competitions and won fourteen national first awards and one second award in only three periods of national artistic exhibition. And noticeably, the chorus of Shenzhen Middle School has won the gold award of Olympic Chorusing Competition for continuously three years.<br /><br />
<img src="images/school.jpg" /><br />
<br />
</p></td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td>&nbsp;</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
</table><br />
<table width="100%" border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="245"><img src="https://static.igem.org/mediawiki/2013hs/c/c7/Buttom_01.jpg" width="245" height="166" /></td><br />
<td background="https://static.igem.org/mediawiki/2013hs/5/52/Buttom_02.jpg"><table width="10%" border="0" align="center" cellpadding="0" cellspacing="0"><br />
<tr><br />
<td><img src="https://static.igem.org/mediawiki/2013hs/4/4e/Buttom_03.jpg" /></td><br />
</tr><br />
</table></td><br />
<td width="330"><img src="https://static.igem.org/mediawiki/2013hs/3/3e/Buttom_04.jpg" /></td><br />
</tr><br />
</table><br />
</body><br />
</html></div>
Limu