Team:Lambert GA/labnotebook

From 2013hs.igem.org

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<td>June 11th</td>
<td>June 11th</td>
<td>We ran a gradient PCR with HybB and R0011 as control. diagnostic gel showed the best temperature for PCR was 44.6°C and 53.3°C. On the gel we had bands around 400 base pairs, which is the expected length of our primers and part. We used QIAprep for PCR cleanup. We decided to combine 4 PCR reactions and purify to use in digestions and ligations. After purification HybB is 27.4ng/ul and control was 9.9 ng/ul.
<td>We ran a gradient PCR with HybB and R0011 as control. diagnostic gel showed the best temperature for PCR was 44.6°C and 53.3°C. On the gel we had bands around 400 base pairs, which is the expected length of our primers and part. We used QIAprep for PCR cleanup. We decided to combine 4 PCR reactions and purify to use in digestions and ligations. After purification HybB is 27.4ng/ul and control was 9.9 ng/ul.
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<center>https://static.igem.org/mediawiki/2013hs/b/bc/June11Gel.jpg</center>
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[[File:June11Gel.jpg]]
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<center>June 11 Gel</center>
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Revision as of 17:55, 21 June 2013