Team:Lambert GA/labnotebook

From 2013hs.igem.org

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<td>May 30th</td>
<td>May 30th</td>
<td>Several of our members attended a safety training meeting, and are now certified in lab safety courses.</td>
<td>Several of our members attended a safety training meeting, and are now certified in lab safety courses.</td>
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<td>June 3rd</td>
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<td>Designed primers and developed lab calendar</td>
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<td>June 4th</td>
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<td>Ordered primers</td>
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<td>June 6th</td>
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<td>We researched HybB and PSB1C3. Then chloramphenicol plates were made.</td>
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<td>June 7th</td>
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<td>We did a practice PCR reaction and ran a diagnostic gel to check practice our PCR. Primers came in, so we made stock solutions of a 1/10 dilution and a 1/20 dilution from the primers.</td>
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<td>June 10th</td>
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<td>We did a PCR of our HybB promoter and ran a gel. The gel showed that the temperature of our PCR was incorrect. Then we ran a gradient PCR with HybB promoter at deven temperatures ranging from 44.6°C to 53.3°C with the control at 54.5°C.
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Then we inoculated liquid stock with DH10 beta in order to purify genomic DNA.</td>
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<td>June 11th</td>
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<td>We ran a diagnostic gel, which showed the best temperature for PCR was 46.1°C and 53.3°C. On the gel we got 500 base pairs, which is the expected length of our primers and part. We used QIAprep for PCR cleanup.</td>
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<td>June 12th</td>
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<td>We met with Georgia Tech iGem team and heard their presentation and discussed cooperation and outreach. We did a double digest with EcoRl remix and PST1. We blasted all of our parts to check all of the DNA sequences against the parts registry. We also worked on the wiki and the poster.</td>
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<td>June 13th</td>
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<td>We repeated the double digest. We used Antarctic phosphatase to cut off the extra phosphates on the 5 prime ends of the backbone. We ligated the HybB promoter and the backbone and we included a control of just the backbone. Then we transformed the ligation mixtures of today and yesterday resulting in eight plates.</td>
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Revision as of 18:47, 14 June 2013