| Date | Accomplishments |
|---|---|
| January 1-15 | Met for the first time during class time as an Instructional Focus. Discussed project ideas and iGEM in general. Gave out protocols to study |
| January 16-31 | Practiced transformations with p-GLO from Biorad. Learned about Bacterial Antibiotic resistance using Biorad Antibiotic Resistance Lab. |
| February 1-14 | Discussed project ideas. Planned for visit to Georgia Institute of Technology, Styczynski Lab. Sold Pretzels for fund raiser. |
| February 15-28 | Studied and took test over iGEM protocols and 3A Assembly. Finalized list of Biobrick parts of interest. Outlined initial ideas. Use K410000 to characterize cold shock heat generator. |
| March 1-14 | Field trip to GA Tech on March 9 • Rehydrated J23119, J13002, J04450, K410000 • Miniprepped parts • Ran diagnostic gels • Sent parts for sequencing • Calculated concentration of DNA • Met with team members from GATech iGEM. Discussed the K410000 project. • New idea for project; isolate HybB and make it a Biobrick. GATech team will send their frozen stocks. |
| March 15-28 | Worked on research for the new parts. Wrote Project proposal. |
| April 1-24 | April 1st • Transformed again • Made Amp plates • Cataloged Gaucher 2010 box • Plated the HypB with RFP on Amp plates April 4th • Liquid culture results were analyzed • Took the HypB samples and mixed with Glycerol (Glyceroled Bio Bricks) and placed them in 80oC freezer • Streaked LB plates with HypB samples, promoter, and K410000 (bio brick) • Made more LB plates • Miniprepped hypB parts and K410 from Gaucher box • Calculated the DNA concentration • Calculated concentrations for sequencing • Wrote instructions for Cold Shock April 24th • Nanodropped with DNA concentrations • Ligation ( Protocol) • Digest of RFP, hypB, psBIC3, 410 |
| May 1- May 14 | • Transformation • RFP control and 410 RFP grew but only RFP has an expression • Ran transformation efficiency test with Protocol • Prep for experiment- plated 10 plates and inoculated 16 tubes • Experiment for temperature variation was successful but did not prove hypothesis |
| May 30th | Several of our members attended a safety training meeting, and are now certified in lab safety courses. |
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