Team:Shenzhen SFLS/Project

From 2013hs.igem.org

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===Project===
===Project===
Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP, students could simply order it or extract it from a plant. For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does. This can be achieved by: 
Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP, students could simply order it or extract it from a plant. For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does. This can be achieved by: 
 +
1.Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast genome
1.Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast genome
 +
2.Order this gene from a laboratory.
2.Order this gene from a laboratory.
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3.Insert(插入) this gene into e。coil or yeast.
3.Insert(插入) this gene into e。coil or yeast.
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4.Isolate(隔离) AP after it has been expressed.
4.Isolate(隔离) AP after it has been expressed.
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5. Use fluorometric  analysis(荧光分析) and note the baseline wavelength(排列波长) of light shot through a agarose/jellose/AP in the presence of an acid.
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5. Use fluorometric analysis(荧光分析)and note the baseline wavelength(排列波长) of light shot through a agarose/jellose/AP in the presence of an acid.
===Team===
===Team===

Revision as of 08:48, 25 March 2013

iGEM-2012HS - LSL-Heidelberg


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have the following information on your wiki:
  • a team description
  • project description
  • safety information (did your team take a safety training course? were you supervised in the lab?)
  • team attribution (who did what part of your project?)
You may also wish to add other page such as:
  • lab notebook
  • sponsor information
  • other information
REMEMBER, keep all of your pages within your teams namespace.
Example: 2013hs.igem.org/Team:Shenzhen_SFLS/Our_Pets

Project

Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP, students could simply order it or extract it from a plant. For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does. This can be achieved by: 

1.Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast genome

2.Order this gene from a laboratory.

3.Insert(插入) this gene into e。coil or yeast.

4.Isolate(隔离) AP after it has been expressed.

5. Use fluorometric analysis(荧光分析)and note the baseline wavelength(排列波长) of light shot through a agarose/jellose/AP in the presence of an acid.

Team

blablabla


Notebook

Show us how you spent your days.


Results/Conclusions

What did you achieve over the course of your semester?


Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?


Attributions

Who worked on what?


Human Practices

What impact does/will your project have on the public?


Fun!

What was your favorite team snack?? Have a picture of your team mascot?


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