Team:Shenzhen SFLS/Project

From 2013hs.igem.org

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Revision as of 15:25, 29 March 2013

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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

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a team description
project description
safety information (did your team take a safety training course? were you supervised in the lab?)
team attribution (who did what part of your project?)

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lab notebook
sponsor information
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Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	Your team picture
	Team Shenzhen_SFLS

Official Team Profile

Student at SFLS want to produce an enzyme known as “polyphosphate kinase”（PPK）to governance the eutrophication of water bodies. We know that the eutrophication of water bodies is often caused by Pi. If the the concentration of phosphate in the water is too high, ther will have a lot of truble such as red tide in lake, sea and river.PPK is known to digest Pi (phosphate). We want to make two device in a gene circut to deal with this problem.

De0vice 1: We use a limitable promoter which would be limited by high concentrations of Pi, then the GFP in this device won't light and a protein we add in the device won't breed too.

Device 2: If the protein didn't breed, the promoter in Device 2 will be induced and the PPK gene will work, it will digest Pi. When the concentration of phosphate become low, devive 2 will stop to digest Pi and the promoter in device 1 will be induced, then the GFP in device 1 will light. That means the work has finished.

( We will put on our picture of gene circuts in few days)

Notebook

Show us how you spent your days.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

Who worked on what?

Human Practices

What impact does/will your project have on the public?

Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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 ===Project===
-Student at SFLS want to produce an enzyme known as “acid phosphatase” (“AP”). AP is known to be inhibited by heavy metals, and it might be inhibited by different pesticides. In order to attain AP,　students could simply order it or extract it from a plant.　For IGEM, however, they will need to do it the hard way: they will need to force a bacteria to produce more AP than it normally does.　This can be achieved by:
+Student at SFLS want to produce an enzyme known as “polyphosphate kinase”（PPK）to governance the eutrophication of water bodies. We know that the eutrophication of water bodies is often caused by Pi. If the the concentration of phosphate in the water is too high, ther will have a lot of truble such as red tide in lake, sea and river.PPK is known to digest Pi (phosphate). We want to make two device in a gene circut to deal with this problem.
-１．Find out what gene is responsible for AP production. Figure out if it can be easily inserted into e.coli or yeast　genome.
+De0vice 1: We use a limitable promoter which would be limited by high concentrations of Pi, then the GFP in this device won't light and a protein we add in the device won't breed too.
-２．Order this　gene from a　laboratory．
+Device 2: If the protein didn't breed, the promoter in Device 2 will be induced and the PPK gene will work, it will digest Pi. When the concentration of phosphate become low, devive 2 will stop to digest Pi and the promoter in device 1 will be induced, then the GFP in device 1 will light. That means the work has finished.
-３．Insert this gene into　e.coil　or yeast.
-４．Isolate AP　after it has been expressed.
+( We will put on our picture of gene circuts in few days)
-５． Use　fluorometric ａｎａｌｙｓｉｓ ａｎｄ ｎｏｔｅ ｔｈｅ baseline　ｗａｖｅｌｅｎｇｔｈoｆｌｉｇｈｔ　ｓｈｏｔ　ｔｈｒｏｕｇｈ　ａ　agarose／jellose／ＡＰ in the presence of an acid.
-Add different heavy metals or pesticides to this mixture and do other fluorometric analyses. These analyses should yield different wavelengths because AP activity is inhibited by heavy metals, etc.
 ===Notebook===