http://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&feed=atom&action=historyTeam:Shenzhen SFLS/Project - Revision history2024-03-28T22:04:48ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=14553&oldid=prevEricLee at 02:35, 22 June 20132013-06-22T02:35:48Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Result</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>After we finished our project, we started to test it. We test two part of our project. The first one is our Device One. We tested its response to Pi starvation. Here is the results diagram.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>In this diagram we can find that Device one can be induced by phosphate starvation. When the concentration of dipotassium phosphate is 0.03μM we can see that our sensor reached a peak because our promoter can be activated by Pi starvation.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>Next is our whole Device. We tested when the green fluorescence would vanish and red fluorescence occur. Here is another diagram.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>This diagram shows that after several hours the amount of green fluorescence decreases, which means the work has been done and red fluorescence will occur.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>This result clearly pointed out that our product can work smoothly. The Pi starvation promoter is working well too. Our experiments have reached a success at last.</p</ins></div></td></tr>
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</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=12474&oldid=prevEricLee at 16:42, 21 June 20132013-06-21T16:42:12Z<p></p>
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</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=11466&oldid=prevEricLee at 00:36, 21 June 20132013-06-21T00:36:55Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We found its genetic <del class="diffchange diffchange-inline">circuit </del>and we used PCR to made it a standard biobrick. Then we started to find the promoters of our product. Finally we found the Pi Starvation Promoter(BBa_K737023), it’s not on the kits so we again designed its primer and used PCR.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>We found its genetic <ins class="diffchange diffchange-inline">sequence </ins>and we used PCR to made it a standard biobrick. Then we started to find the promoters of our product. Finally we found the Pi Starvation Promoter(BBa_K737023), it’s not on the kits so we again designed its primer and used PCR.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For the protein we used TetR protein(BBa_C0040)</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For the protein we used TetR protein(BBa_C0040)</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Now it’s time for our final genetic circuit. Take a look!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Now it’s time for our final genetic circuit. Take a look!</div></td></tr>
</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=10283&oldid=prevEricLee at 10:01, 20 June 20132013-06-20T10:01:12Z<p></p>
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</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=10282&oldid=prevEricLee at 10:00, 20 June 20132013-06-20T10:00:58Z<p></p>
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</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=10280&oldid=prevEricLee at 09:58, 20 June 20132013-06-20T09:58:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In this page, you can see how it was designed and made.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In this page, you can see how it was designed and made.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Eutrophication can be a very severe problem to the environment. In an effort to solve this problem, we want to build a genetic system which can test and remove the phosphate from water. In our idea, we want to make a product which can automatically detects phosphate and therefore remove it. And it must be a totally environmentally friendly one. And we began our designing.<del class="diffchange diffchange-inline"><br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Eutrophication can be a very severe problem to the environment. In an effort to solve this problem, we want to build a genetic system which can test and remove the phosphate from water. In our idea, we want to make a product which can automatically detects phosphate and therefore remove it. And it must be a totally environmentally friendly one. And we began our designing.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/d/d9/Project_img_2.jpg" width="800" height="284" alt="" /> <del class="diffchange diffchange-inline"><br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/d/d9/Project_img_2.jpg" width="800" height="284" alt="" /> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Frist we designed a genetic circuit which can test how the work has been done. We wanted to find a promoter which can only be induced by Pi starvation. Then there is a suppressor protein and an <span>rfp</span>. A double terminator is at the end of this device. This device can only work when the concentration of Pi is low. And the water will show red fluorescence. The red can also be a signal of ‘STOP’, which means the work has already finished.<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Frist we designed a genetic circuit which can test how the work has been done. We wanted to find a promoter which can only be induced by Pi starvation. Then there is a suppressor protein and an <span>rfp</span>. A double terminator is at the end of this device. This device can only work when the concentration of Pi is low. And the water will show red fluorescence. The red can also be a signal of ‘STOP’, which means the work has already finished.<br /></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Next we designed a second device for removing the phosphate. The promoter of this device is repressed by the protein in Device one, which means when the concentration of Pi is low, Device two will be limited and stop working. Then we wanted to find a CDS which can remove phosphate from water. And a <span>gfp</span>+LVA(degradation tag) with a terminator at the end. When this device is working, The CDS will remove the phosphate from water and meanwhile the gfp+LVA will show green fluorescence, just like the green traffic signal. This device is mainly used for removing phosphate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Next we designed a second device for removing the phosphate. The promoter of this device is repressed by the protein in Device one, which means when the concentration of Pi is low, Device two will be limited and stop working. Then we wanted to find a CDS which can remove phosphate from water. And a <span>gfp</span>+LVA(degradation tag) with a terminator at the end. When this device is working, The CDS will remove the phosphate from water and meanwhile the gfp+LVA will show green fluorescence, just like the green traffic signal. This device is mainly used for removing phosphate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>This photo can show you how the two device are linked<del class="diffchange diffchange-inline"><br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>This photo can show you how the two device are linked</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/8/87/Project_img_4.jpg" width="800" height="525" alt="" <del class="diffchange diffchange-inline">/> <br </del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/8/87/Project_img_4.jpg" width="800" height="525" alt="" /> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>When we put our product into the water which has a very high concentration of phosphate, Device one will be limited and Device two will be activated. Then the water will show green fluorescence which means our product is removing Pi.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>When we put our product into the water which has a very high concentration of phosphate, Device one will be limited and Device two will be activated. Then the water will show green fluorescence which means our product is removing Pi.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/b/bf/Project_img_5.jpg" width="800" height="458" alt="" <del class="diffchange diffchange-inline">/><br </del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/b/bf/Project_img_5.jpg" width="800" height="458" alt="" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After a period of time, due to Device two’s work, the concentration of Pi decreases, then Device one will be activated because of Pi starvation. The suppressor protein will repress Device two. Device one will work and show red fluorescence to tell us that the work has been finished.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After a period of time, due to Device two’s work, the concentration of Pi decreases, then Device one will be activated because of Pi starvation. The suppressor protein will repress Device two. Device one will work and show red fluorescence to tell us that the work has been finished.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>That’s the idea of our project, we then started searching for files that support our idea. First we searched for a CDS which can digest phosphate. Then after weeks of searching we found Polyphosphate Kinase, also known as PPK. It is a kind of enzyme widely distributed in ecosystem, more importantly, it can be found in E-coli. Here this picture below can show you how it works.<del class="diffchange diffchange-inline"><br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>That’s the idea of our project, we then started searching for files that support our idea. First we searched for a CDS which can digest phosphate. Then after weeks of searching we found Polyphosphate Kinase, also known as PPK. It is a kind of enzyme widely distributed in ecosystem, more importantly, it can be found in E-coli. Here this picture below can show you how it works.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/c/cd/Project_img_6.jpg" width="800" height="298" alt="" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/c/cd/Project_img_6.jpg" width="800" height="298" alt="" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We found its genetic circuit and we used PCR to made it a standard biobrick. Then we started to find the promoters of our product. Finally we found the Pi Starvation Promoter(BBa_K737023), it’s not on the kits so we again designed its primer and used PCR.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We found its genetic circuit and we used PCR to made it a standard biobrick. Then we started to find the promoters of our product. Finally we found the Pi Starvation Promoter(BBa_K737023), it’s not on the kits so we again designed its primer and used PCR.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For the protein we used TetR protein(BBa_C0040)</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For the protein we used TetR protein(BBa_C0040)</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Now it’s time for our final genetic circuit. Take a look!<del class="diffchange diffchange-inline"><br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Now it’s time for our final genetic circuit. Take a look!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/7/72/Project_img_7.jpg" width="800" height="262" alt="" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/7/72/Project_img_7.jpg" width="800" height="262" alt="" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=10277&oldid=prevEricLee at 09:33, 20 June 20132013-06-20T09:33:13Z<p></p>
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</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=10275&oldid=prevEricLee at 09:28, 20 June 20132013-06-20T09:28:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Next we designed a second device for removing the phosphate. The promoter of this device is repressed by the protein in Device one, which means when the concentration of Pi is low, Device two will be limited and stop working. Then we wanted to find a CDS which can remove phosphate from water. And a <span>gfp</span>+LVA(degradation tag) with a terminator at the end. When this device is working, The CDS will remove the phosphate from water and meanwhile the gfp+LVA will show green fluorescence, just like the green traffic signal. This device is mainly used for removing phosphate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Next we designed a second device for removing the phosphate. The promoter of this device is repressed by the protein in Device one, which means when the concentration of Pi is low, Device two will be limited and stop working. Then we wanted to find a CDS which can remove phosphate from water. And a <span>gfp</span>+LVA(degradation tag) with a terminator at the end. When this device is working, The CDS will remove the phosphate from water and meanwhile the gfp+LVA will show green fluorescence, just like the green traffic signal. This device is mainly used for removing phosphate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>This photo can show you how the two device are linked</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>This photo can show you how the two device are linked<ins class="diffchange diffchange-inline"><br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img src="https://static.igem.org/mediawiki/2013hs/8/87/Project_img_4.jpg" width="800" height="525" alt="" /> <br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">When we put our product into the water which has a very high concentration of phosphate, Device one will be limited and Device two will be activated. Then the water will show green fluorescence which means our product is removing Pi.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img src="https://static.igem.org/mediawiki/2013hs/b/bf/Project_img_5.jpg" width="800" height="458" alt="" /><br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">After a period of time, due to Device two’s work, the concentration of Pi decreases, then Device one will be activated because of Pi starvation. The suppressor protein will repress Device two. Device one will work and show red fluorescence to tell us that the work has been finished.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>That’s the idea of our project, we then started searching for files that support our idea. First we searched for a CDS which can digest phosphate. Then after weeks of searching we found Polyphosphate Kinase, also known as PPK. It is a kind of enzyme widely distributed in ecosystem, more importantly, it can be found in E-coli. Here this picture below can show you how it works.<br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img src="https://static.igem.org/mediawiki/2013hs/c/cd/Project_img_6.jpg" width="800" height="298" alt="" /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>We found its genetic circuit and we used PCR to made it a standard biobrick. Then we started to find the promoters of our product. Finally we found the Pi Starvation Promoter(BBa_K737023), it’s not on the kits so we again designed its primer and used PCR.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>For the protein we used TetR protein(BBa_C0040)</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>Now it’s time for our final genetic circuit. Take a look!<br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img src="https://static.igem.org/mediawiki/2013hs/7/72/Project_img_7.jpg" width="800" height="262" alt="" /></ins></div></td></tr>
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</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=10270&oldid=prevEricLee at 09:17, 20 June 20132013-06-20T09:17:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In this page, you can see how it was designed and made.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In this page, you can see how it was designed and made.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Eutrophication can be a very severe problem to the environment. In an effort to solve this problem, we want to build a genetic system which can test and remove the phosphate from water. In our idea, we want to make a product which can automatically detects phosphate and therefore remove it. And it must be a totally environmentally friendly one. And we began our designing.<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Eutrophication can be a very severe problem to the environment. In an effort to solve this problem, we want to build a genetic system which can test and remove the phosphate from water. In our idea, we want to make a product which can automatically detects phosphate and therefore remove it. And it must be a totally environmentally friendly one. And we began our designing.<br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/d/d9/Project_img_2.jpg" width="800" height="284" alt="" /> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/d/d9/Project_img_2.jpg" width="800" height="284" alt="" /> <ins class="diffchange diffchange-inline"><br /></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Frist we designed a genetic circuit which can test how the work has been done. We wanted to find a promoter which can only be induced by Pi starvation. Then there is a suppressor protein and an <span>rfp</span>. A double terminator is at the end of this device. This device can only work when the concentration of Pi is low. And the water will show red fluorescence. The red can also be a signal of ‘STOP’, which means the work has already finished.<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Frist we designed a genetic circuit which can test how the work has been done. We wanted to find a promoter which can only be induced by Pi starvation. Then there is a suppressor protein and an <span>rfp</span>. A double terminator is at the end of this device. This device can only work when the concentration of Pi is low. And the water will show red fluorescence. The red can also be a signal of ‘STOP’, which means the work has already finished.<br /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/e/e2/Project_img_3.jpg" width="800" height="244" alt="" /> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/e/e2/Project_img_3.jpg" width="800" height="244" alt="" /> <ins class="diffchange diffchange-inline"><br /></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Next we designed a second device for removing the phosphate. The promoter of this device is repressed by the protein in Device one, which means when the concentration of Pi is low, Device two will be limited and stop working. Then we wanted to find a CDS which can remove phosphate from water. And a <span>gfp</span>+LVA(degradation tag) with a terminator at the end. When this device is working, The CDS will remove the phosphate from water and meanwhile the gfp+LVA will show green fluorescence, just like the green traffic signal. This device is mainly used for removing phosphate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Next we designed a second device for removing the phosphate. The promoter of this device is repressed by the protein in Device one, which means when the concentration of Pi is low, Device two will be limited and stop working. Then we wanted to find a CDS which can remove phosphate from water. And a <span>gfp</span>+LVA(degradation tag) with a terminator at the end. When this device is working, The CDS will remove the phosphate from water and meanwhile the gfp+LVA will show green fluorescence, just like the green traffic signal. This device is mainly used for removing phosphate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
</table>EricLeehttp://2013hs.igem.org/wiki/index.php?title=Team:Shenzhen_SFLS/Project&diff=10269&oldid=prevEricLee at 09:17, 20 June 20132013-06-20T09:17:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013hs/6/6b/Project_img_0.jpg" width="939" height="2702" alt="Project" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2013hs/6/6b/Project_img_0.jpg" width="939" height="2702" alt="Project" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>--></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>This is our project, EcoPi. </<del class="diffchange diffchange-inline">p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>This is our project, EcoPi. <<ins class="diffchange diffchange-inline">br </ins>/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/4/41/Project_img_1.jpg" width="800" height="262" alt="" /> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013hs/4/41/Project_img_1.jpg" width="800" height="262" alt="" /> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In this page, you can see how it was designed and made.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In this page, you can see how it was designed and made.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Eutrophication can be a very severe problem to the environment. In an effort to solve this problem, we want to build a genetic system which can test and remove the phosphate from water. In our idea, we want to make a product which can automatically detects phosphate and therefore remove it. And it must be a totally environmentally friendly one. And we began our designing.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Eutrophication can be a very severe problem to the environment. In an effort to solve this problem, we want to build a genetic system which can test and remove the phosphate from water. In our idea, we want to make a product which can automatically detects phosphate and therefore remove it. And it must be a totally environmentally friendly one. And we began our designing.<ins class="diffchange diffchange-inline"><br /></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img src="https://static.igem.org/mediawiki/2013hs/d/d9/Project_img_2.jpg" width="800" height="284" alt="" /> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Frist we designed a genetic circuit which can test how the work has been done. We wanted to find a promoter which can only be induced by Pi starvation. Then there is a suppressor protein and an <span>rfp</span>. A double terminator is at the end of this device. This device can only work when the concentration of Pi is low. And the water will show red fluorescence. The red can also be a signal of ‘STOP’, which means the work has already finished.<br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><img src="https://static.igem.org/mediawiki/2013hs/e/e2/Project_img_3.jpg" width="800" height="244" alt="" /> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Next we designed a second device for removing the phosphate. The promoter of this device is repressed by the protein in Device one, which means when the concentration of Pi is low, Device two will be limited and stop working. Then we wanted to find a CDS which can remove phosphate from water. And a <span>gfp</span>+LVA(degradation tag) with a terminator at the end. When this device is working, The CDS will remove the phosphate from water and meanwhile the gfp+LVA will show green fluorescence, just like the green traffic signal. This device is mainly used for removing phosphate.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>This photo can show you how the two device are linked</p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
</table>EricLee