Team:TPHS SanDiego/Project

From 2013hs.igem.org

(Difference between revisions)
Line 1: Line 1:
 +
<html>
<html>
 +
<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013hs/3/35/TorrePinesNavigation%28Tareq%29.jpg" alt="header" border="0"  usemap="#igemmap" width="1000"></center></div>
 +
 +
<map name="igemmap">
 +
  <area  shape="circle" coords="480,40,40" alt="Mercury" href="https://2011.igem.org" onfocus="this.blur()" />
 +
</map>
 +
 +
 +
<div class="layout-1000">
 +
<div class="row">
 +
<div class="col12" >
 +
<ul class="topnav">
 +
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego">Home  </a></li>
 +
    <li>
 +
        <a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Project">Project</a>
 +
    </li>
 +
    <li>
 +
        <a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Parts">Parts</a>
 +
    </li>
 +
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Safety">Safety</a></li>
 +
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Team">Team</a></li>
 +
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Protocols">Protocols</a></li>
 +
    <li><a href="https://2013hs.igem.org/Team:TPHS_SanDiego/Journal">Journal</a></li>
 +
</ul>
 +
<div> <div class ="row-end"> </div>
 +
</div>
 +
</div>
 +
 +
<div class="row">
 +
<div class="col12" id="spacer2"></div>
 +
<div class="row-end"> </div>
 +
</div>
 +
 +
<div class="row">
 +
<div class="col12-2"> <img src="https://static.igem.org/mediawiki/2011/1/19/Teambanner-berkeley.jpg" width="980"> </div>
 +
<div class="row-end" ></div>
 +
</div>
 +
</html>

Revision as of 18:03, 18 June 2013

header
Mercury


Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter.

Retrieved from "http://2013hs.igem.org/Team:TPHS_SanDiego/Project"