Team:TPHS SanDiego/Project

From 2013hs.igem.org

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</html>
</html>
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<html lang="en">
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<style = "text/css" media="screen">
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background-color: gray;
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ul.topnav li span.subhover {background-position: center bottom; cursor: pointer;} /*--Hover effect for trigger--*/
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ul.topnav li ul.subnav {
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font-family: 'Georgia', Times, serif;
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font-weight: 150;
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html ul.topnav li ul.subnav li a {
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float: left;
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width: 145px;
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}
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html ul.topnav li ul.subnav li a:hover { /*--Hover effect for subnav links--*/
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background-color: blue;
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}
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font-family:Georgia ,Helvetica,sans-serif;
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height: 300px;
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font-family:Georgia ,Helvetica,sans-serif;
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height: 50px;
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body{ background: url(https://static.igem.org/mediawiki/2013hs/f/f4/BackgroundTareq.jpg);}
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#content{
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padding: 0px;
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width: 1000px;
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background: #ffe5d4;
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// Background Color
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font-family:Georgia ,Helvetica,sans-serif;
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border: none;
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text-decoration: none;
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}
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.main_item h1{
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text-decoration:none;
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display:none;
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#top-section2{
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width: 100%;
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background: url(http://www.eecs.berkeley.edu/~jvmiller/images/imagetestberkeley.gif);
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height: 65px;
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    height: 20px;
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#team{border: 1px solid black;
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padding: 2px;
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height:380px;}
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    height: 12em;
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/*
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* jQuery Nivo Slider v2.6
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* http://nivo.dev7studios.com
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*
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* Copyright 2011, Gilbert Pellegrom
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* Free to use and abuse under the MIT license.
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* http://www.opensource.org/licenses/mit-license.php
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*
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* March 2010
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*/
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position:relative;
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z-index:6;
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display:none;
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display:block;
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position:absolute;
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z-index:5;
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height:100%;
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display:block;
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position:absolute;
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z-index:5;
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/* Caption styles */
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.nivo-caption {
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position:absolute;
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left:0px;
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bottom:0px;
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background:#000;
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color:#fff;
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width:100%;
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z-index:8;
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.nivo-caption p {
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padding:5px;
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.nivo-caption a {
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display:inline !important;
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}
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.nivo-html-caption {
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    display:none;
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}
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/* Direction nav styles (e.g. Next & Prev) */
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.nivo-directionNav a {
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position:absolute;
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top:45%;
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z-index:9;
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cursor:pointer;
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}
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.nivo-prevNav {
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left:15px;
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}
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.nivo-nextNav {
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right:15px;
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}
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/* Control nav styles (e.g. 1,2,3...) */
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.theme-default .nivo-controlNav {
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      position:absolute; left:100%; bottom:-42px;
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      margin-left: -1000px;
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.nivo-controlNav a {
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position:relative;
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z-index:9;
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cursor:pointer;
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}
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.nivo-controlNav a.active {
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font-weight:bold;
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}
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.theme-default #slider {
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    height:464px; /* Make sure your images are the same size */
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}
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</style>
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</html>

Revision as of 17:55, 18 June 2013


Our project focuses on promoter engineering. Our goal is to characterize a set of promoters (of our design) by moving the repressor and/or activator binding sites with respect to the -10 and -35 regions of the promoter. Ideally, we would like to show that by moving an activator binding site it can become a repressor and that by moving a repressor binding site it may become either irrelevant to transcription rate or even boost it. We also want to see if there is a steep decline in repressor/activator function as the binding site move along the promoter or if it is a gradual/linear change. We believe this project could have application to genetic circuits by allowing a single protein to either activate or repress a promoter depending on where the binding sites are placed on the promoter.

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