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From 2013hs.igem.org

Put protocol here! CPEC Wobble EIPCR (enzymatic inverse PCR) Normal ligation assembly (3A assembly) Mini-preps Transformations Ligations Digestions Normal PCRs Gel-extractions PCR-purifications Running electrophoresis gels Comp cells Plating Overnight cultures (liquid cultures) Denote when the UCSD services had to be used – sequencing, designing primers, data managing techniques, ordering primers, thermocycler