Team:CIDEB-UANL Mexico/WetLab-Notebook
From 2013hs.igem.org
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We did MiniPreparation of Plasmidic DNA of the 3 reporters GFP, YFP, CFP and the 2 plasmids pSB1K3 and pSB1C3. We ran the electrophoresis gel with the 5 DNAs. | We did MiniPreparation of Plasmidic DNA of the 3 reporters GFP, YFP, CFP and the 2 plasmids pSB1K3 and pSB1C3. We ran the electrophoresis gel with the 5 DNAs. | ||
Also, we transformed the synthetic DNAs that just arrived today that are in the PCCI and PUC-57 plasmids. Also this day, we pick up some colonies from the samples of the 5-14O, 5-4E and 5-14I dishes; which were the same reporters that the ones used in the MiniPrep, but these samples were from previous samples then were from the past iGem team. These were stored in the incubator. </p> | Also, we transformed the synthetic DNAs that just arrived today that are in the PCCI and PUC-57 plasmids. Also this day, we pick up some colonies from the samples of the 5-14O, 5-4E and 5-14I dishes; which were the same reporters that the ones used in the MiniPrep, but these samples were from previous samples then were from the past iGem team. These were stored in the incubator. </p> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2013hs/f/f5/Snapshot.jpg" height="300px" width="400px" /> | ||
+ | <p>Image 1. Bacteria with GFP under the microscope.<p></center> | ||
<p align="justify"><b>June 4th, 2013</b><br> | <p align="justify"><b>June 4th, 2013</b><br> | ||
We got a lot of colonies of PCCI and very few of the PUC-57. As the colonies with the cI biobrick did not grow, we transformed it again and a little more of the PUC-57. We also cloned cells to make competent cells.This day we noticed that the colonies of reporters that we picked up the day before didn’t grow. Again this day the colonies of GFP, CFP and YFP and also the PCC1 and PUC-57 were picked up and inserted in other tubes with other LB medium, and again were stored in the incubator. </p> | We got a lot of colonies of PCCI and very few of the PUC-57. As the colonies with the cI biobrick did not grow, we transformed it again and a little more of the PUC-57. We also cloned cells to make competent cells.This day we noticed that the colonies of reporters that we picked up the day before didn’t grow. Again this day the colonies of GFP, CFP and YFP and also the PCC1 and PUC-57 were picked up and inserted in other tubes with other LB medium, and again were stored in the incubator. </p> |
Revision as of 02:23, 22 June 2013
Wet-Lab
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Notebook
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May, 23th, 2013 May, 24th, 2013 May, 29th, 2013 May, 30th, 2013 May, 31th, 2013 Image 1. Bacteria with GFP under the microscope. June, 1st, 2013 June 3rd, 2013 Image 2. Finally we have bacteria in tubes. We are ready to do our first miniPrep Image 1. Bacteria with GFP under the microscope. June 4th, 2013 June 5th, 2013 June 6th, 2013 Image 2. Gel with the MiniPreps June 6th, 2013 June 7th, 2013 Image 3. Gel with the MiniPreps June 6th degradated June 10th, 2013 June 11th, 2013 Image 4. Gel with only PCC1. First time it worked. June 12th, 2013 June 13th, 2013 June 14th, 2013 June 17th, 2013 June 18th, 2013 June 20th, 2013 June 21th, 2013 |
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CIDEB UANL Team. Centro de Investigación y Desarrollo de Educación Bilingüe |
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