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From 2013hs.igem.org

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Contents 1 1 March 2 8 March 2.1 Electrocortecoli 2.2 Thermobacter 2.3 Mineral-Fuser Bacter 3 14 March 3.1 Project Ideas 4 15 March 5 22 March

1 March

-Today marks the official start of our 2013 iGEM adventure. We hope that today becomes a start for the many great things to come. -The reason why today was the "official" start is because our first trip to Turgut Ozal University for this year was today. We met with Mustafa Semih Elitok, our advisor, to discuss the current situation and our project ideas. -The 2 new members of the team got acquinted with our advisor.

-Out of today's discussions, we have decided to work on the following project ideas:

• Cyanide Disposing Bacteria - Cemal and Abdulkadir will work on this idea.
• Miner Bacteria - Alperen and Furkan Sacit will work on this idea.
• Thermobacter - Fatih will work on this idea.
• Musilage Bacter - İbrahim and Lashynbek will work on this idea.

8 March

-We continued discussing our project ideas.

-As a result of the improvising, we decided to conduct research on only these 3 ideas for now:

Electrocortecoli

The E.coli that we plan to produce will be negatively charged. Combined with an adesion molecule, the E.coli will bind to a specific cell thus creating an overall negatively charged system. This system can then be easily destroyed in the presence of a charged unit. Through this, sepcific cells can be targeted with E.coli and then be easily destroyed.

The list of the research required for us to do is as follows:

• Finding the sequence of an adesion molecule which can be implantabled to E.coli
• Finding a way to electrocharge our E.coli
• Searching iGEM to see if something which could be of use was done before

Thermobacter

The thermobacter will be able to sense temperature and react to it through producing urease which will cause the urea we add into the environment to be converted to uric acid. This reaction is endothermic and will result in the environment cooling down. Through this system, an automatic cooler through bacteria will be formed.

The list of the research required for us to do is as follows:

• Searching to see if this project was don before or if there is anything in iGEM that could be of use
• Finding the sequence for urease enzyme
• Designating several ententities of the RNA Thermometer which can be of use

Mineral-Fuser Bacter

14 March

-Today we discussed:

• Project Ideas
• Sponsorship
• Our Previous iGEM Experience

Project Ideas

• Electrocortecoli: We were not able to discuss much about it as we hadn't been successful in finding information. We know the existence of an adhesion molecule for mammal cells in iGEM called cadherin but it is for mammal cells. Also, we still have no clue on how to electrocharge our E.coli.

• Thermobacter: This project looks like it is in the bag. The parts are already in iGEM (RNA Thermometer and urease enzyme). We won't have to worry much about the research for the RNA Thermometer.

• Mineral-Fuser Bacter: The research we conducted over the last week showed that it will be extremely difficult to do this as a project as there is already a lack of information on this matter. The only thing that we could find was that a certain type of bacteria has this property but scientists haven't identified how they do it.

• A Revival of our old Idea Stone Miner: In a multiple choice question of an exam, Furkan Beştepe read about a type of coral called "Lithophaga". This coral was found in Naples and had been designated as the cause of the degrading of historical buildings. This coral can eat through stone!

15 March

-The discussions we had in school yesterday were pretty much carried out but this time with our advisor. -Also, we did our new tradition once more. What is this you may ask. Well it is 2 minutes of random idea burst without reasoning. Here are the results:

• Neurobacter, Painkiller, Writer, Clock-o-Bacter, Methanecoli, Rust Eater, Decayer, Tooth Paste, Tooth Filling, Balloon Inflator, CO Filter, Imnosia Bacter, SleepingBactuety, Photosynthetic, Fire Figther, Quartz Watch, Tire Repair, Anti-Bacterial-Bacter, UV to Visible Light Converter, Barocoli, Cooler, Optic Glass, Virus Detector, Infrared Bacter, Jammer, Psychic Bacter, Crystalecoli, Balance, Sewing Bacter, MRBacter, Pixellence, Glass Eater, Asphalt 4, Vacuum, Sun Lotion, Painter, Hemoglobin Detector, PCR, Anti-Radioctivity Bacter, Bactomembrane, Glue Bacter, Plastic Eater, Paper Consumer

22 March

-As of today, considering the time and difficulty, we decided to focus only on Thermobacter. To improve thermobacter, we realized that we had add at least one part to iGEM. As a new part we could do:

1) A New Type of RNA Thermometer

2) Another addition to the project

-With the remaining time, we came up with human practice ideas.

• Visiting schools
• Visiting businessman
• Visiting corporations
• Conducting surveys
• Preparing handouts
• Doing advertisements
• iGEMan
• Making a board game
• Making a computer game
• Preparing Canvas Times 2
• Doing Videos