Team:CIDEB-UANL Mexico

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:CIDEB-UANL_Mexico_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:CIDEB-UANL_Mexico | Team CIDEB-UANL_Mexico]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=CIDEB-UANL_Mexico Official Team Profile]
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===Team===
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Tell us about your team, your school!
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Project</div>
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Abstract
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===Project Description===
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What are you working on this semester?
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<p><b>Thermonator III: The crop guardian</b></p>
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<p align="justify">Our project is about a genetic engineered machine in <i>E. coli</i> with the ability to produce Vip3ca3 which acts as a pesticide protein. Vip3ca3 production will be regulated by specific temperatures in order to avoid overproduction and it will show activity against target organisms Coleoptera and Lepidoptera, which are related to a local problem concerning potato crops. The Vip3ca3 production is regulated with a constitutive promoter and a riboswitch that initiate translation around 32°C. Since we want to produce the Vip3ca3 below the 32°C, we use a set of promoter-repressors in order to invert the activation of the protein production. This model may be used as a regulator in future transgenic plant generations for the production of substances against plagues, avoiding pesticide overproduction, thus reducing the effect in Non-Target Organisms and bioaccumulation and can be used with different temperature ranges and/or different proteins to attack other target organisms.</p>
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The agriculture is a very important activity and is the base of our alimentation and the production of raw materials that eventually will be transformed into products or more complicated materials. Different technologies have been applied to agriculture to reduce important problems and some obstacles that decrease the efficiency in the cultivation of certain plants.  One problem that affects considerably some cultures is the presence of plagues. 
 
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Some insects cause a significant damage to cultures and reduce the quality of the products. In order to avoid or reduce the impact of these organisms, the use of pesticides has increased and it is very common in the cultivation of almost all plants. Nevertheless, the use of these substances causes damage in the environment by the contamination of water and soil.
 
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Nowadays we can find Genetically-Modified plants adapted to avoid these problems such as the GM maize: “The GM maize planted in Europe produces a substance that enables it to defend itself against a persistent pest known as the European corn borer.”[http://www.gmo-compass.org/eng/grocery_shopping/crops/18.genetically_modified_maize_eu.htm 1]
 
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Our idea is about a genetically modified bacterium which produces a biological pesticide (Vip3ca3) against target organisms: earthworms. The bacterium would be regulated by temperature and aims to control pesticide production. As it is a biological pesticide it does not harm the environment and affects only target species reducing the impact this could have in such environment and its effects on other species.
 
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1.GMO Compass. (2008, December 3). Genetically modified maize in the EU. Retrieved from http://www.gmo-compass.org/eng/grocery_shopping/crops/18.genetically_modified_maize_eu.htm
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===Notebook===
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Show us how you spent your days.
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===Results/Conclusions===
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What did you achieve over the course of your semester?
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===Safety===
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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===Attributions===
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Who worked on what?
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===Human Practices===
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What impact does/will your project have on the public? 
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===Fun!===
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What was your favorite team snack?? Have a picture of your team mascot?
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Revision as of 20:54, 21 June 2013

Project
Abstract

Thermonator III: The crop guardian

Our project is about a genetic engineered machine in E. coli with the ability to produce Vip3ca3 which acts as a pesticide protein. Vip3ca3 production will be regulated by specific temperatures in order to avoid overproduction and it will show activity against target organisms Coleoptera and Lepidoptera, which are related to a local problem concerning potato crops. The Vip3ca3 production is regulated with a constitutive promoter and a riboswitch that initiate translation around 32°C. Since we want to produce the Vip3ca3 below the 32°C, we use a set of promoter-repressors in order to invert the activation of the protein production. This model may be used as a regulator in future transgenic plant generations for the production of substances against plagues, avoiding pesticide overproduction, thus reducing the effect in Non-Target Organisms and bioaccumulation and can be used with different temperature ranges and/or different proteins to attack other target organisms.

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