Team:CIDEB-UANL Mexico/Math-Parameters

From 2013hs.igem.org

(Difference between revisions)
(Created page with "<h2>Parameters and variables</h2> <p>Our gene circuit is made of six different variables: the concentrations of three proteins (cI, VIP and GFP) and their respective mRNA inside...")
Line 1: Line 1:
-
<h2>Parameters and variables</h2>
+
{{:Team:CIDEB-UANL_Mexico/style}}
 +
{{:Team:CIDEB-UANL_Mexico/menu}}
 +
<html>
 +
<head>
 +
<link href='http://fonts.googleapis.com/css?family=PT+Sans+Narrow' rel='stylesheet' type='text/css' />
 +
<style>
-
<p>Our gene circuit is made of six different variables: the concentrations of three proteins (cI, VIP and GFP) and their respective mRNA inside a cell. In table 1, the symbols for each variable are shown. Proteins are represented by a single letter and their mRNAs are represented by that same letter with a lowercase "m" before it.</p>
+
#texto {
 +
float: left;
 +
background-color: #FFFFFF;
 +
font-family: 'PT Sans Narrow';
 +
font-size:18px;
-
<table border="6" cellpadding="1" cellspacing="1">
+
}
-
<caption>Table 1.- Variables</caption>
+
-
<tr>
+
-
<td>Symbol</td>
+
-
<td>Definition</td>
+
-
<td>Gene size in bp</td>
+
-
<td>Source</td>
+
-
</tr>
+
-
<tr>
+
-
<td BGCOLOR="#2E64FE">mC, C</td>
+
-
<td BGCOLOR="#2E64FE">Transcription factor cI (mRNA and protein)</td>
+
-
<td>775</td>
+
-
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051">http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td BGCOLOR="#BF00FF">mV, V</td>
+
-
<td BGCOLOR="#BF00FF">Insecticide protein VIP (mRNA and protein)</td>
+
-
<td>2412</td>
+
-
<td><a href="http://www.ncbi.nlm.nih.gov/nuccore/HQ876489">http://www.ncbi.nlm.nih.gov/nuccore/HQ876489</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td BGCOLOR="#01DF01">mG, G</td>
+
-
<td BGCOLOR="#01DF01">Reporter protein GFP (mRNA and protein)</td>
+
-
<td>876</td>
+
-
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_E0240">http://parts.igem.org/wiki/index.php?title=Part:BBa_E0240</a></td>
+
-
</tr>
+
-
</table>
+
 +
.Estilo6 {
 +
        font-family: 'PT Sans Narrow';
 +
        color: #FFFFFF;
 +
}
 +
h1 {height:55px; padding-left: 10px; background-color: #0A2A12; font-size:50px; color:white; font-weight:bold; text-decoration:none; text-align:left;}
 +
h3 {height:15px; padding-left: 10px; background-color: #0B3B24; font-size:30px; color:white; font-weight:bold; text-decoration:none; text-align:left;}
 +
p {padding: 14px;}
-
<p>To parameterize our model, we chose to follow the approach of team Beijing 2009; they propose a relationship between the gene length in base pairs and the maximum transcription rate and, similarly, between the protein length in amino acid numbers and the maximum translation rate. Assuming that the number of polymerases and ribosomes are the average values determined for E. coli, the following equations are used:</p>
+
</style>
-
<table border="6" cellpadding="2" cellspacing="2">
+
</head>
-
<caption>Parameters</caption>
+
 
 +
<body>
 +
 
 +
<div style="background-color: #0B3B24;" class="Estilo6">
 +
 
 +
<h1>Project</h1></div>
 +
<div>
 +
<h3>Abstract</h3>
 +
</div>
 +
<h4></h4>
 +
 
 +
</div>
 +
 
 +
<div style="background-color: #FFFFFF;">
 +
<table width="100%" id="texto">
<tr>
<tr>
-
<th>Symbol</th>
+
<td>
-
<th>Definition</th>
+
<p><b>Thermonator III: The crop guardian</b></p>
-
<th>Values</th>
+
<p align="justify">Our project is about a genetic engineered machine in E. coli with the ability to produce Vip3ca3 which acts as a pesticide protein. Vip3ca3 production will be regulated by specific temperatures in order to avoid overproduction and it will show activity against target organisms Coleoptera and Lepidoptera, which are related to a local problem concerning potato crops. The Vip3ca3 production is regulated with a constitutive promoter and a riboswitch that initiate translation around 32°C. Since we want to produce the Vip3ca3 below the 32°C, we use a set of promoter-repressors in order to invert the activation of the protein production. This model may be used as a regulator in future transgenic plant generations for the production of substances against plagues, avoiding pesticide overproduction, thus reducing the effect in Non-Target Organisms and bioaccumulation and can be used with different temperature ranges and/or different proteins to attack other target organisms.</p>
-
<th>Formula</th>
+
</td>
-
<th>Source</th>
+
<td style="background-color: #FFFFFF;">
-
</tr>
+
<img src="https://static.igem.org/mediawiki/2013hs/d/d3/D9b5b18226b32476e3e16622aae4da9e.jpg" height="300px" />
-
<tr>
+
</td>
-
<td>α1</td>
+
-
<td>Transcription rate of cI</td>
+
-
<td>5.6</td>
+
-
<td>4200/Gene Length (nM/min)</td>
+
-
<td><a href="https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters">https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td>α2</td>
+
-
<td>Translation rate of cI</td>
+
-
<td>9.6</td>
+
-
<td>2400RBS/Protein Length</td>
+
-
<td><a href="https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters">https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td>α3</td>
+
-
<td>Transcription rate of VIP</td>
+
-
<td>1.74129353</td>
+
-
<td>4200/Gene Length (nM/min)</td>
+
-
<td><a href="https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters">https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td>α4</td>
+
-
<td>Translation rate of VIP</td>
+
-
<td>2.985075</td>
+
-
<td>2400RBS/Protein Length</td>
+
-
<td><a href="https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters">https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td>α5</td>
+
-
<td>Transcription rate of GFP</td>
+
-
<td>5.53359684</td>
+
-
<td>4200/Gene Length (nM/min)</td>
+
-
<td><a href="https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters">https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td>α6</td>
+
-
<td>Translation rate of GFP</td>
+
-
<td>9.486166</td>
+
-
<td>2400RBS/Protein Length</td>
+
-
<td><a href="https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters">https://2009.igem.org/Team:PKU_Beijing/Modeling/Parameters</a></td>
+
</tr>
</tr>
</table>
</table>
 +
</div>
-
<p>For all the variables the degradation rate is expressed by the formula (ln(2)/half life)+(ln(2)/division time), with the same division time of e. coli (30 min), because all the process occurs within it. The only thing that change is the half time; for cI, VIP and GFP (mRNA) is 6.8 minutes and for cI (Selinger, GW, et al., 2003), VIP and GFP protein is more than 10 hours (Varshavsky, (1997) and Tobias et al., 1991).</p>
 
-
<table border="6" cellpadding="2" cellspacing="2">
+
</body>
-
<caption>Degradation</caption>
+
 
-
<tr>
+
</html>
-
<th>Symbol</th>
+
{{:Team:CIDEB-UANL_Mexico/footer}}
-
<th>Definition</th>
+
-
<th>Values</th>
+
-
<th>Formula</th>
+
-
<th>Source</th>
+
-
</tr>
+
-
<tr>
+
-
<td>μ1μ3,μ5,</td>
+
-
<td>Degradation rate of cI (mRNA</td>
+
-
<td>0.18063836</td>
+
-
<td>Half life = 6.8 min, Division time = 30 min</td>
+
-
<td>(Selinger, GW, et al., 2003)</td>
+
-
</tr>
+
-
<tr>
+
-
<td>μ2,μ4,μ6</td>
+
-
<td>Degradation rate of cI (protein)</td>
+
-
<td>0.03885825</td>
+
-
<td>Half life > 10 h; division time = 30 min</td>
+
-
<td>(Varshavsky, (1997) and Tobias et al., 1991)</td>
+
-
</tr>
+
-
</table>
+

Revision as of 04:01, 16 June 2013

Project

Abstract

Thermonator III: The crop guardian

Our project is about a genetic engineered machine in E. coli with the ability to produce Vip3ca3 which acts as a pesticide protein. Vip3ca3 production will be regulated by specific temperatures in order to avoid overproduction and it will show activity against target organisms Coleoptera and Lepidoptera, which are related to a local problem concerning potato crops. The Vip3ca3 production is regulated with a constitutive promoter and a riboswitch that initiate translation around 32°C. Since we want to produce the Vip3ca3 below the 32°C, we use a set of promoter-repressors in order to invert the activation of the protein production. This model may be used as a regulator in future transgenic plant generations for the production of substances against plagues, avoiding pesticide overproduction, thus reducing the effect in Non-Target Organisms and bioaccumulation and can be used with different temperature ranges and/or different proteins to attack other target organisms.

cideb
cideb
Contact us! Follow us on twitter and facebook or send us a mail.
CIDEB UANL Team. Centro de Investigación y Desarrollo de Educación Bilingüe
facebooktwitterenvelope