Team:CIDEB-UANL Mexico/Project-Preview

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<p><b>How do we get to this project?</b></p>
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<p align="justify">After the workshop given to us, we started with a brainstorm to have ideas from where we could buid a project. We started with some ideas, such as bacteria with the ability to break down DDT; bacteria that detected cancerous cells and killed them; bacteria that produced alliinase in order to catalyze the formation of allicin which is a substance produced by <i>Allium Sativum</i> (garlic) and has pesticide properties[1].</p>
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In September, 2013, the school invited all the 3th semester students to participate in a selection in order to create the team IGEM-CIDEB 2013. A big conference was planned and a lot of students went in order to know a more about the competition and Synthetic Biology.</p>
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The filters consisted in 2 phases. The first one was a series of classes with information about Synthetic Biology during October and November. The classes were given by 2 of the instructors.  In these classes, each week was taught a topic about Synthetic Biology and it was given an assignment for each topic.</p>
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<p align="justify"> We liked that idea but we found a lot of complications to get the project done, somewhere that allicin is a very volatile substance, so capturing it was very difficult, <i>E. coli</i> but allicin has anti-bacterial properties, and other properties that didn’t result favorable for our project[2]. Due to these problems, we decided to look for another substance but we wanted to work with the same idea of pesticides. <br>We found some proteins with pesticide properties that were discovered few years ago and that do not harm the environment. One of them is the Vip3 protein which has a lot of derives. Some of them where more problematic for our project idea than others, for this, research had to be continued. A big problem we had to face is that the Vip3 is about 3000 bp and this is a big size for a protein to be inserted in the plasmids we would be working with.  Finally, we choose the substance Vip3ca3 and that’s how we got to the actual project but this is only the first part of the whole project idea.</p></td>
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<p align="justify">The second phase was about a workshop in the laboratory with practices such as transformation, miniPreparation of DNA plasmidic, electrophoresis and some others, during the second week of January 2013.
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Based on the participation, assistance and compromise, the final team was selected. After the workshop we started with a brainstorm of ideas about the project. We started with some ideas such as a bacterium with the ability to breakdown DDT, a bacterium that detected cancerous cells and kill them, a bacterium that produce alliinase in order to catalyze the formation of allicin which is a substance produced by the Allium Sativum (garlic) and has pesticide properties1. We liked the idea of the alliinase but we found a lot of implications to get the project done. Some of the obstacles were that allicin is a very volatile substance, that we wanted to work with E. coli but allicin has anti-bacterial properties, and other small complications2. Due to these problems, we decided to look for another substance but we wanted to work with the same idea of the pesticides. </p>
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<p align="justify">We found some proteins with pesticide properties that were discovered few years ago and that do not harm the environment. One of them is the Vip3 protein which has a lot of derivatives. We were finding also more obstacles with some of the substances with wanted to work, so we had to continue with the researching. A big problem we had to face is that the Vip3 is about 3000 bp and this is a big size for a protein to be inserted in the plasmids we would be working in.  Finally, we choose the substance Vip3ca3 and that’s how we get to the actual project but this is only the first part of the project idea.</p>
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1 Chavarrías, Marta. "Ajo en la lucha contra Campylobacter."Eroski Consumer. Fundación Eroski contigo, 16 May 2012. Web. 23 May 2013. <http://www.consumer.es/seguridad-alimentaria/ciencia-y-tecnologia/2012/05/16/209583.php>.
1 Chavarrías, Marta. "Ajo en la lucha contra Campylobacter."Eroski Consumer. Fundación Eroski contigo, 16 May 2012. Web. 23 May 2013. <http://www.consumer.es/seguridad-alimentaria/ciencia-y-tecnologia/2012/05/16/209583.php>.
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2 EB Kuettner et al, The active principle of garlic at atomic resolution, J. Biol. Chem. 277 (2002) 46402-46407
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<br>2 EB Kuettner et al, The active principle of garlic at atomic resolution, J. Biol. Chem. 277 (2002) 46402-46407
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Latest revision as of 06:47, 22 June 2013

Project
Preview

After the workshop given to us, we started with a brainstorm to have ideas from where we could buid a project. We started with some ideas, such as bacteria with the ability to break down DDT; bacteria that detected cancerous cells and killed them; bacteria that produced alliinase in order to catalyze the formation of allicin which is a substance produced by Allium Sativum (garlic) and has pesticide properties[1].

We liked that idea but we found a lot of complications to get the project done, somewhere that allicin is a very volatile substance, so capturing it was very difficult, E. coli but allicin has anti-bacterial properties, and other properties that didn’t result favorable for our project[2]. Due to these problems, we decided to look for another substance but we wanted to work with the same idea of pesticides.
We found some proteins with pesticide properties that were discovered few years ago and that do not harm the environment. One of them is the Vip3 protein which has a lot of derives. Some of them where more problematic for our project idea than others, for this, research had to be continued. A big problem we had to face is that the Vip3 is about 3000 bp and this is a big size for a protein to be inserted in the plasmids we would be working with. Finally, we choose the substance Vip3ca3 and that’s how we got to the actual project but this is only the first part of the whole project idea.

1 Chavarrías, Marta. "Ajo en la lucha contra Campylobacter."Eroski Consumer. Fundación Eroski contigo, 16 May 2012. Web. 23 May 2013. .
2 EB Kuettner et al, The active principle of garlic at atomic resolution, J. Biol. Chem. 277 (2002) 46402-46407

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