Team:Consort Alberta/humanpract

From 2013hs.igem.org

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Revision as of 21:18, 21 June 2013

In order to educate our community about our project and the benefits of synthetic biology, we started out by talking to our local businesses. Our first objective was to introduce the ideas of non-toxic E. coli bacteria, and the benefits of genetic engineering. Every business owner expressed a vested interest in the idea of genetic engineering, especially when we revealed our project idea of measuring the amounts of xylene that had escaped into the soil around lease land, and was brought up into plants through root systems. Our local oil company (called 3-Star) was especially interested, and was the first to be interviewed on their opinions on the matter of using synthetic biology being utilized to identify hydrocarbon contamination. Though the business’ representatives hadn’t heard a lot about the new field of synthetic biology they agree that it is an extremely useful science and has endless applications in improving the oilfield. Not only did we talk to local people, we interviewed an oilfield remediation specialist in Calgary for a better idea of just how important avoiding environmental contamination really is.

Consort is a community build on a sturdy foundation of oil and agriculture. Unfortunately however, our oil industry will sometimes overlap with the agriculture industry in the form of lease lands and oil extraction. A lease land is an area of land the oil company rents from a farmer in order to access the oil resources deep in the soil. This means that the sites of oil extraction are in extremely close proximity with the food we all end up buying in the grocery store. With benzene and benzene derivatives being unhealthy for our cattle, crops and consumers, ranchers have become wary about allowing oil leases in their pastures. Detecting the hydrocarbons will be our first step to identifying an oil spill and if there are dangerous hydrocarbons contaminating produce - oil spills vary in regard to dangerous content. As there is a correlation between the amount of xylene and the amount of benzene in an area, we have created the plans for E. coli bacteria to measure the amount of xylene and indirectly the amount of benzene. This means that the beef we all buy for consumption at the supermarket can potentially contain contaminants. This class of compounds (benzene and benzene derivatives) is able to intercalate into DNA, causing mutations and is carcinogenic as a result. With dangerous hydrocarbons being so close to our cattle and crops - our main food supply - it becomes a dangerous safety consideration for both farmers and oil industry workers to take into account. By introducing the idea of E. coli xylene detectors we have found a cost effective and efficient potential safety method for the wellbeing of businesses and consumers alike.

As our community is built on a foundation of oil and agriculture we felt this was the best way to support our town, and our local businesses associated with these industries agreed wholeheartedly. Mid-June, we invited community members to join us in the lab so we could show them what we had been up to and practice presenting. Though it was only our first time presenting, the feedback was positive and encouraging and our village representatives were full of helpful constructive criticism that helped us build on what we had so far to better represent our remote community.We didn't only talk to business leaders in our village. In June, we presented our project to the grade four class, explaining to them our project and the generalities of altering DNA to serve a different function. We created a different presentation for the occasion that explained the concepts in a simplified manner, and we're thrilled at the enthusiasm with which it was received. Consort's elementary is now full of little iGem enthusiasts who can't wait to get to high school.

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-<p> Consort is an oil and agriculture based community. Our oil industry will sometimes overlap with the agriculture industry in the form of lease lands and oil extraction. A lease land is an area of land the oil company rents from a farmer in order to access the oil resources deep in the soil. This means that the sites of oil extraction are in extremely close proximity with the food we all end up buying in the grocery store.  With hydrocarbons being unhealthy for our cattle, crops and consumers, ranchers have become wary about allowing oil leases in their pastures. Detecting the hydrocarbons will be our first step to identifying an oil spill and if there are dangerous hydrocarbons contaminating produce. Hydrocarbons in the environment are extremely detrimental as it has been linked to cancer and blood problems in lab tests. With hydrocarbons being so close to our cattle and crops - our main food supply - it becomes a dangerous safety consideration for both farmers and oil industry workers to take into account.
+<p> In order to educate our community about our project and the benefits of synthetic biology, we started out by talking to our local businesses. Our first objective was to introduce the ideas of non-toxic E. coli bacteria, and the benefits of genetic engineering. Every business owner expressed a vested interest in the idea of genetic engineering, especially when we revealed our project idea of measuring the amounts of xylene that had escaped into the soil around lease land, and was brought up into plants through root systems. Our local oil company (called 3-Star) was especially interested, and was the first to be interviewed on their opinions on the matter of using synthetic biology being utilized to identify hydrocarbon contamination. Though the business’ representatives hadn’t heard a lot about the new field of synthetic biology they agree that it is an extremely useful science and has endless applications in improving the oilfield. Not only did we talk to local people, we interviewed an oilfield remediation specialist in Calgary for a better idea of just how important avoiding environmental contamination really is.
 <br><br>
-When we first told our community that we were dealing with E. Coli bacteria, the first thing people thought of was the E. coli found in an XL food plant in Canada. It caused 18 illnesses in Canada, leading to the largest food recall in Canadian history. So once we finished telling them that there are all different kinds of E. coli they found what we were doing useful and interesting
-<br><br> In order to educate our community about our project and the benefits of synthetic biology, we started out by talking to our local businesses. Our first objective was to introduce the ideas of non-toxic e. coli bacteria, and the benefits of genetic engineering. Every business owner expressed a vested interest in the idea of genetic engineering, especially when we revealed our project idea of measuring the amounts of xylene that had escaped into the soil around lease land, and was brought up into plants through root systems. Our local 3-Star oil company was especially interested, and was the first to be interviewed on their opinions on the matter of using synthetic biology being utilized to identify hydrocarbon contamination. Though the business’ representatives hadn’t heard a lot about the new field of synthetic biology they agree that it is an extremely useful science and has endless applications in improving the oilfield.
+ Consort is a community build on a sturdy foundation of oil and agriculture. Unfortunately however, our oil industry will sometimes overlap with the agriculture industry in the form of lease lands and oil extraction. A lease land is an area of land the oil company rents from a farmer in order to access the oil resources deep in the soil. This means that the sites of oil extraction are in extremely close proximity with the food we all end up buying in the grocery store.  With benzene and benzene derivatives being unhealthy for our cattle, crops and consumers, ranchers have become wary about allowing oil leases in their pastures. Detecting the hydrocarbons will be our first step to identifying an oil spill and if there are dangerous hydrocarbons contaminating produce - oil spills vary in regard to dangerous content. As there is a correlation between the amount of xylene and the amount of benzene in an area, we have created the plans for E. coli bacteria to measure the amount of xylene and indirectly the amount of benzene. This means that the beef we all buy for consumption at the supermarket can potentially contain contaminants.  This class of compounds (benzene and benzene derivatives) is able to intercalate into DNA, causing mutations and is carcinogenic as a result. With dangerous hydrocarbons being so close to our cattle and crops - our main food supply - it becomes a dangerous safety consideration for both farmers and oil industry workers to take into account.  By introducing the idea of E. coli xylene detectors we have found a cost effective and efficient potential safety method for the wellbeing of businesses and
-<br><br>
+consumers alike.
-The fact that lease lands are in the middle of our fields means the grain we eat is potentially contaminated. Furthermore, the cows that graze in fields surrounding lease land eat grasses that may have absorbed contaminants. This means that the beef we buy for consumption at the supermarket can potentially contain contaminants as well.  As exposure to xylene is linked to cancer and blood problems, this isn't a good thing.
-<br><br>
-As our community is built on a foundation of oil and agriculture we felt this was the best way to support our town, and our local businesses associated with these industries agreed wholeheartedly. Mid-June, we invited community members to join us in the lab so we could show them what we had been up to and practice presenting. Though it was only our first time presenting, the feedback was positive and encouraging, and our village representatives were full of helpful constructive criticism that helped us build on what we had so far to better represent our remote community.
-<br><br>
-We didn't only talk to business leaders in our village. In June, we presented our project to the grade four class, explaining to them our project and the generalities of altering DNA to serve a different function. We created a different presentation for the occasion that explained the concepts in a simplified manner, and we're thrilled at the enthusiasm with which it was received. Consort's elementary is now full of little iGem enthusiasts who can't wait to get to high school. Not only did we talk to local people, we interviewed a Consort Alberta iGEM 2013
 <br><br>
-The aim of this year’s Consort high-school iGEM project is to engineer a bacterial strain which can be used to test for xylene levels in soil samples. We live in a rural area and our economy is based on agriculture and fossil fuel production. The presence of certain hydrocarbon compounds can pose health and safety risks if they enter the food chain or if people exceed safe exposure limits. Oil and gas wells are routinely drilled on leases, small portions of fields used for agricultural production. Oil is usually trucked from these sites to collection centres where it is processed. Every time the oil is transferred there is an opportunity for some spillage to occur. Not all spills, however, are equally dangerous and differentiating between more and less dangerous spills is important in reacting to them appropriately. Additionally the extent or the presence of a dangerous spill is often uncertain. Our project would, in principle, allow anyone to easily and quickly answer all the queries above. Early identification of contamination will facilitate rapid clean-up and minimize health risks to members of our community and to the consumers who rely on the food we produce.
+As our community is built on a foundation of oil and agriculture we felt this was the best way to support our town, and our local businesses associated with these industries agreed wholeheartedly. Mid-June, we invited community members to join us in the lab so we could show them what we had been up to and practice presenting. Though it was only our first time presenting, the feedback was positive and encouraging and our village representatives were full of helpful constructive criticism that helped us build on what we had so far to better represent our remote community.We didn't only talk to business leaders in our village. In June, we presented our project to the grade four class, explaining to them our project and the generalities of altering DNA to serve a different function. We created a different presentation for the occasion that explained the concepts in a simplified manner, and we're thrilled at the enthusiasm with which it was received. Consort's elementary is now full of little iGem enthusiasts who can't wait to get to high school.
-<br><br>
+</p>
-Our project has been the development of ECOS (Environmental COntaminant Sensor). The heart of the sensor is an E. coli culture that has been modified to produce green fluorescent protein when exposed to xylene. Xylene was chosen as a trigger because its presence is very well correlated with the presence of other more dangerous compounds such as benzene and benzene derivatives. This class of compounds is able to intercalate into DNA, causing mutations and is carcinogenic as a result. The XylR transcriptional activator is a protein which in the presence of m-xylene will bind to the Pu promoter resulting in the expression of our reporter, green fluorescent protein. The other components of the sensor include a heated sample container to assist in vapourizing the xylene and an air pump to push the vapour through our bacterial culture.