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Team:CIDEB-UANL Mexico/WetLab-Results
From 2013hs.igem.org
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<img src="https://static.igem.org/mediawiki/2013hs/0/0d/Imagen_circuito.png" width="500px" height="350px" /></p> | <img src="https://static.igem.org/mediawiki/2013hs/0/0d/Imagen_circuito.png" width="500px" height="350px" /></p> | ||
| - | <p align="justify">We had a lot of problems with the part "PCC1" because there were problems when it was </p> | + | <p align="justify">We had a lot of problems with the part "PCC1" because there were problems when it was synthesized. We couldn't cut it but we tried to ligate it. The part "PUC-57" worked very well while cutting with the restriction enzymes and at the ligation. For the Friday 21th, 2013, we got colonies of both ligations. We think the "PCC1" is not a good ligation because it did not released the fragment. But we are going to characterize the part with the "PUC-57" the next week. We cannot be sured right now because it doesn't have a reporter.</p> |
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Revision as of 00:15, 22 June 2013
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Wet-Lab
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Results
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Here there is a construction map of the circuit:
We had a lot of problems with the part "PCC1" because there were problems when it was synthesized. We couldn't cut it but we tried to ligate it. The part "PUC-57" worked very well while cutting with the restriction enzymes and at the ligation. For the Friday 21th, 2013, we got colonies of both ligations. We think the "PCC1" is not a good ligation because it did not released the fragment. But we are going to characterize the part with the "PUC-57" the next week. We cannot be sured right now because it doesn't have a reporter. |
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