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| - | <div id="notebook_march">
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| - | <div id="wrapper">
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| - | <div id="header">
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| - | <a href="https://2013hs.igem.org/Team:Lethbridge_Canada">
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| - | <div id="logo_all">
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| - | <img src="https://static.igem.org/mediawiki/2013hs/3/32/Leth_hs_2013_logo.png" alt="logo" />
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| - | <span id="title_first">Lethbridge</span>
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| - | <span id="title_second">iGEM Team</span>
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| - | </a>
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| - | <div id="cont">
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| - | <div class="cleafix>">
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| - | <div id="navigation">
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| - | <li class="navigation_project"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project" class="strict">Project</a>
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| - | <ul>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Description</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/math">Math Model</a></li>
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| - | </ul>
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| - | </li>
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| - | <li class="navigation_parts decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/parts" class="strict">Parts</a></li>
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| - | <li class="navigation_notebook decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols" class="strict">Notebook</a>
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| - | <ul>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols#protocol_header">Protocols</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">Notebook: March</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april">Notebook: April</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may">Notebook: May</a></li>
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| - | </ul>
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| - | </li>
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| - | <li class="navigation_safety decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/safety" class="strict">Safety</a></li>
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| - | <li class="navigation_outreach decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach" class="strict">Outreach</a>
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| - | <ul>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach">Overview</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/presentations">Presentations</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/interviews">Interviews</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/videos">Videos</a></li>
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| - | </ul>
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| - | <li class="navigation_team decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team" class="strict">Team</a>
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| - | <ul>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_team">The Team</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_advisors">The Advisors</a></li>
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| - | </ul>
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| - | </li>
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| - | <li class="navigation_sponsors decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/sponsors" class="strict">Sponsors</a></li>
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| - | </ul>
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| - | </div>
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| - | </div>
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| - | <div id="mainContent">
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| - | <div id="notebook_header">
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| - | <div id="notebook_links">
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| - | <h1 id="notebook_links_header">Notebook Links</h1>
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| - | <ul id="notebook_march_links">
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| - | <li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">March</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_one">March 12, 2013: Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_two">March 13, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_three">March 13, 2013: Miniprep and Glycerol Stock</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_four">March 15, 2013: Restriction Digestion</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_five">March 16, 2013: Agarose Gel</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_six">March 18, 2013: Agarose Gel</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_seven">March 19, 2013: Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_eight">March 20, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_nine">March 21, 2013: Miniprep and Glycerol Stock</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_ten">March 27, 2013: Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_eleven">March 28, 2013: Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_twelve">March 28, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_thirteen">March 29, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_fourteen">March 29, 2013: Picking Cells of -80 Glycerol Stock</a></li>
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| - | </ul>
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| - | <ul id="notebook_april_links">
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| - | <li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april">April</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_one">April 2, 2013: Restriction - Ligation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_two">April 2, 2013: Ligation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_three">April 3, 2013: Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_four">April 4, 2013: Re-Plating Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_five">April 5, 2013: Miniprep of ON Cultures</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_six">April 5, 2013: UV Spectroscopy DNA Concentrations</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_seven">April 6, 2013: Miniprep From Ligations</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_eight">April 7, 2013: PCR</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_nine">April 8, 2013: Restriction Digestion of PCR Parts</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_ten">April 8, 2013: 3% Agarose Gel of Digested PCR Parts</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_eleven">April 8, 2013: Gel Extraction</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twelve">April 9, 2013: Repeat PCR</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_thirteen">April 9, 2013: Picking Cell Colonies (Cultures)</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_fourteen">April 10, 2013: Miniprep</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_fifteen">April 10, 2013: Restriction</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_sixteen">April 10, 2013: Agarose Gel of PCR 1%</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_seventeen">April 11, 2013: Miniprep</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_eighteen">April 12, 2013: Restriction Digestion</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_nineteen">April 12, 2013: PCR and Cell Reculturization</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty">April 12, 2013: Miniprep</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_one">April 14, 2013: Miniprep</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_two">April 14, 2013: 1% Agarose Gel</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_six">April 15, 2013: Restriction/Digestion</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_seven">April 15, 2013: Restriction/Digestion</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_three">April 15, 2013: 1% Agarose Gel of Promoters</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_four">April 17, 2013: Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_five">April 30, 2013: 1% Agarose Gel of Promoter Constructs</a></li>
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| - | </ul>
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| - |
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| - | <ul id="notebook_may_links">
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| - | <li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may">May</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_one">May 1, 2013: Ligation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_two">May 4, 2013: Restriction Digest</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_three">May 4, 2013: 1% Agarose Gel of Promoter Constructs</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_four">May 4, 2013: Gel Extractions</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_five">May 5, 2013: Transformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_six">May 7, 2013: Restriction</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_seven">May 7, 2013: Gel Extraction</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eight">May 7, 2013: Gel Extracted on Conformation Gel</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_nine">May 8, 2013: Ligations</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_ten">May 9, 2013: PCR of Ligation Mixture</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eleven">May 13, 2013: Restriction of PCR Ligations Gel Conformation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twelve">May 14, 2013: Transformation of Ligations</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirteen">May 15, 2013: Ligation</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_fourteen">May 15, 2013: Picking Cells</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_fifteen">May 16, 2013: Picking Cells</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_sixteen">May 16, 2013: 10 Fold Miniprep</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_seventeen">May 17, 2013: Agarose Gel</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eighteen">May 17, 2013: Miniprep</a></li>
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| - | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_nineteen">May 17, 2013: Glycerol Stock</a></li>
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| - | </ul>
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| - | </div>
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| - | </div>
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| - |
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| - |
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| - | <div id="notebook_body">
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| - | <div class="notebook_entry">
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| - | <div id="entry_one">
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| - | <h1 class="notebook_entry_header">Transformations</h1>
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| - | <ul class="notebook_entry_list">
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| - | <li >Name: Marie C, Fiona S, Chris I, Katie T, Wesley M</li>
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| - | <li >Date: March 12, 2013</li>
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| - | <li >Species: E. Coli</li>
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| - | <li >Strain: DH5A</li>
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| - | <li >Protocol: Ute Kothe</li>
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| - | <li >Antibiotic: Kan and Amp</li>
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| - | <li >Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3</li>
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| - | <li >Results: TBA</li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_two">
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| - | <h1 class="notebook_entry_header">Picking Cell Colonies (Cultures)</h1>
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| - | <ul class="notebook_entry_list">
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| - | <li >Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H</li>
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| - | <li >Date: March 13, 2013</li>
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| - | <li >Species: E. Coli</li>
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| - | <li >Strain: DH5A</li>
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| - | <li >Protocol: Ute Kothe</li>
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| - | <li >Antibiotic: Amp</li>
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| - | <li >Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3</li>
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| - | <li >Results: TBA</li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_three">
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| - | <h1 class="notebook_entry_header">Miniprep and Glycerol Stock</h1>
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| - | <ul class="notebook_entry_list">
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| - | <li >Names: Mark H, Chris I, Elaine B, Dax L</li>
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| - | <li >Date: March 13, 2013</li>
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| - | <li >Species: E. Coli</li>
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| - | <li >Strain: DH5A</li>
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| - | <li >Protocol: Ute Kothe</li>
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| - | <li >Miniprepped: 3-B0030, 3-B0031, 3-B0032,
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| - | 2-J04500 and 3-R0010</li>
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| - | <li >Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5</li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_four">
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| - | <h1 class="notebook_entry_header">Restriction Digestion</h1>
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| - | <ul class="notebook_entry_list">
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| - | <li >Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S</li>
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| - | <li >Date: March 15, 2013</li>
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| - | <li >Species: E. Coli</li>
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| - | <li >Strain: DH5A</li>
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| - | <li >Protocol: Ute Kothe use buffer 4 instead of buffer 10</li>
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| - | <li >Restricted: 6-B0030, 6-B0031, 6-B0032,
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| - | 4-J04500 and 6-R0010</li>
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| - | <li >Incubate 1 hour at 37˚ C</li>
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| - | <li >Stored freezer overnight</li>
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| - | </ul>
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| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
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| - | </div>
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| - | </div>
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| - |
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| - | <div class="notebook_entry">
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| - | <div id="entry_five">
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| - | <h1 class="notebook_entry_header">Agarose Gel</h1>
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| - | <div class="restrict_width">
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| - | <ul class="notebook_entry_list">
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| - | <li >Name: Patrick O</li>
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| - | <li >Date: March 16, 2013</li>
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| - | <li >Species: E. Coli</li>
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| - | <li >Strain: DH5A</li>
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| - | <li >Protocol: Ute Kothe</li>
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| - | <li >Ran 15 uL each of 3-B0030, 3-B0031, 3-B0032,
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| - | 2-J04500 and 3-R0010 through 1% agarose gel</li>
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| - | <li >Quantities: 12.5 uL DNA and 2.5 uL of 6x loading dye (15 uL total), 12.5 uL 1kb DNA ladder and 2.5 uL 6x loading dye (15 uL total) note: ladder difficult to see on gel picture, next time less DNA ladder</li>
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| - | <ul>
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| - | </div>
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| - | <table class="table_three table_position">
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| - | <thead>
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| - | <tr>
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| - | <th>Lane #</th>
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| - | <th>Contents</th>
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| - | <th>Volume(µL)</th>
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| - | </tr>
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| - | </thead>
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| - | <tbody>
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| - | <tr>
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| - | <td>1</td>
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| - | <td>Empty</td>
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| - | <td>n/a</td>
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| - | </tr>
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| - | <tr>
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| - | <td>2</td>
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| - | <td>1kb DNA Ladder</td>
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| - | <td>15</td>
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| - | </tr>
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| - | <tr>
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| - | <td>3</td>
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| - | <td>BBa_B0030</td>
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| - | <td>15</td>
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| - | </tr>
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| - | <tr>
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| - | <td>4</td>
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| - | <td>BBa_B0030</td>
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| - | <td>15</td>
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| - | </tr>
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| - | <tr>
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| - | <td>5</td>
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| - | <td>BBa_B0030</td>
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| - | <td>15</td>
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| - | </tr>
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| - | <tr>
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| - | <td>6</td>
| |
| - | <td>BBa_B0031</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>7</td>
| |
| - | <td>BBa_B0031</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>8</td>
| |
| - | <td>BBa_B0031</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>9</td>
| |
| - | <td>BBa_B0032</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>10</td>
| |
| - | <td>BBa_B0032</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>11</td>
| |
| - | <td>BBa_B0032</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>12</td>
| |
| - | <td>BBa_R0010</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>13</td>
| |
| - | <td>BBa_R0010</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>14</td>
| |
| - | <td>BBa_R0010</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>15</td>
| |
| - | <td>BBa_J04500</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>16</td>
| |
| - | <td>BBa_J04500</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>17+</td>
| |
| - | <td>empty</td>
| |
| - | <td>n/a</td>
| |
| - | </tr>
| |
| - | </tbody>
| |
| - | </table>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_six">
| |
| - | <h1 class="notebook_entry_header">Agarose Gel</h1>
| |
| - | <div class="restrict_width">
| |
| - | <ul class="notebook_entry_list restrict_width">
| |
| - | <li >Names: Fiona S, Katie T, Chris I, Elaine B. </li>
| |
| - | <li >Date: March 18, 2013 </li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Ran 15 uL each of 3-B0030, 3-B0031, 3-B0032,
| |
| - | 2-J04500 and 3-R0010 through 1% agarose gel</li>
| |
| - | <li >Quantities: 12.5 uL DNA and 2.5 uL of 6x loading dye (15 uL total), 12.5 uL 1kb DNA ladder and 2.5 uL 6x loading dye (15 uL total)</li>
| |
| - | <li ><img src="https://static.igem.org/mediawiki/2013hs/f/fc/Leth_hs_2013_agarose_picture_3_18_2013.gif" alt"Agrose Gel on March 18, 2013" width="400px" /></li>
| |
| - | </ul>
| |
| - | </div>
| |
| - | <ul>
| |
| - | <li>
| |
| - | <table class="table_three table_position">
| |
| - | <thead>
| |
| - | <tr>
| |
| - | <th>Lane #</th>
| |
| - | <th>Contents</th>
| |
| - | <th>Volume(µL)</th>
| |
| - | </tr>
| |
| - | </thead>
| |
| - | <tbody>
| |
| - | <tr>
| |
| - | <td>1</td>
| |
| - | <td>Empty</td>
| |
| - | <td>n/a</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>2</td>
| |
| - | <td>1 kb DNA Ladder</td>
| |
| - | <td>6</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>2</td>
| |
| - | <td>1 kb DNA Ladder</td>
| |
| - | <td>6</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>3</td>
| |
| - | <td>BBa_B0030</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>4</td>
| |
| - | <td>BBa_B0030</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>5</td>
| |
| - | <td>BBa_B0030</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>6</td>
| |
| - | <td>BBa_B0031</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>7</td>
| |
| - | <td>BBa_B0031</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>8</td>
| |
| - | <td>BBa_B0031</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>9</td>
| |
| - | <td>BBa_B0032</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>10</td>
| |
| - | <td>BBa_B0032</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>11</td>
| |
| - | <td>BBa_B0032</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>12</td>
| |
| - | <td>BBa_R0010</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>13</td>
| |
| - | <td>BBa_R0010</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>14</td>
| |
| - | <td>BBa_R0010</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>15</td>
| |
| - | <td>BBa_J04500</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>16</td>
| |
| - | <td>BBa_J04500</td>
| |
| - | <td>15</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>17+</td>
| |
| - | <td>Empty</td>
| |
| - | <td>n/a</td>
| |
| - | </tr>
| |
| - | </tbody>
| |
| - | </table>
| |
| - | </li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_seven">
| |
| - | <h1 class="notebook_entry_header">Transformations of J04650</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Names: Fiona S, Chris I.</li>
| |
| - | <li >Date: March 19, 2013 </li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Transformed 2 uL of J04650 pSB1AK3 from Spring 2012 Kit Plate 4 C12 into competent cells.</li>
| |
| - | <li >Quantities: 2 uL J04650 pSB1AK3, 200 uL LB media</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_eight">
| |
| - | <h1 class="notebook_entry_header">Picking Cells of J04650</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Names: Yoyo Y, Wesley M</li>
| |
| - | <li >Date: March 20, 2013</li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Picked cells of J04650 from previous day’s transformation</li>
| |
| - | <li >Colonies didn't form properly within either plate, cells were picked with a dab (single colonies)</li>
| |
| - | <li >Quantities: 3x5ml of media</li>
| |
| - | <li >Antibiotic: Ampicillin</li>
| |
| - | <li >Glycerol Stock: R0010 pSB1A2</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_nine">
| |
| - | <h1 class="notebook_entry_header">Miniprep and Glycerol Stock of R0010</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Name: Mark H</li>
| |
| - | <li >Date: March 21, 2013</li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Miniprepped 1-R0010 used 50uL of Elution Buffer</li>
| |
| - | <li >Glycerol Stocked 1-R0010 in G6</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_ten">
| |
| - | <h1 class="notebook_entry_header">Transformations J04650 and B0015</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Name: Yoyo Y</li>
| |
| - | <li >Date: March 27, 2013</li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Transformed 2uL of J04650, and B0015 into 25uL of DH5A cell</li>
| |
| - | <li >Quantities: 2uL of J04650 and 2uL of B0015 added into 25uL of DH5A cells</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_eleven">
| |
| - | <h1 class="notebook_entry_header">Transformations</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Names: Marie C, Fiona S, Chris I</li>
| |
| - | <li >Date: March 28, 2013</li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Transformed 2.5uL of J06702</li>
| |
| - | <li >Plasmid: pSB1A2 </li>
| |
| - | <li >Quantities: 2.5uL of DNA into 100uL of pSB1A2</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_twelve">
| |
| - | <h1 class="notebook_entry_header">Picking Cells of J04650</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Names: Chris I, Fiona S, Marie C</li>
| |
| - | <li >Date: March 28, 2013</li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Picked 3 colonies from March 19, J04650</li>
| |
| - | <li >Picked 1 colony from March 26, J04650</li>
| |
| - | <li >Picked 1 colony from March 27, B0015</li>
| |
| - | <li >5mL cultures with 5uL ampicillin. </li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_thirteen">
| |
| - | <h1 class="notebook_entry_header">Picking Cells of J06702</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Names: Chris I, Fiona S, Marie C</li>
| |
| - | <li >Date: March 29, 2013</li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Picked 2 colonies from March 28, J06702</li>
| |
| - | <li >5mL cultures with 5uL ampicillin</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | <div class="notebook_entry">
| |
| - | <div id="entry_fourteen">
| |
| - | <h1 class="notebook_entry_header">Picking Cells of -80 Glycerol Stock , J61002, J23113, J23108, J23111, J23100</h1>
| |
| - | <ul class="notebook_entry_list">
| |
| - | <li >Names: Chris I, Fiona S, Marie C</li>
| |
| - | <li >Date: March 29, 2013</li>
| |
| - | <li >Species: E. Coli</li>
| |
| - | <li >Strain: DH5A</li>
| |
| - | <li >Protocol: Ute Kothe</li>
| |
| - | <li >Picked, J61002</li>
| |
| - | <li >Picked, J23113</li>
| |
| - | <li >Picked, J23108</li>
| |
| - | <li >Picked, J23114</li>
| |
| - | <li >Picked, J23100 </li>
| |
| - | <li >5mL cultures with 5uL ampicillin</li>
| |
| - | <li >Shaken overnight at 37 degrees Celsius</li>
| |
| - | </ul>
| |
| - | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| - | </body>
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| - | </html>
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