Team:CIDEB-UANL Mexico/Project-Abstract

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<p><b>Thermonator III: The crop guardian</b></p>
<p><b>Thermonator III: The crop guardian</b></p>
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<p align="justify">Nuestro proyecto es sobre una máquina geneticamente diseñada en E.coli con la habilidad de producir Vip3ca3, una proteína bio-insecticida. La producción del Vip3ca3 será regulada por temperaturas específicas para controlar la producción y atacar mejor a ciertos organismos en específico. Esta proteína sólo ataca lombrices como Chrysodeixis chalcites y Helicoverpa armigera. La producción del Vip3ca3 se regula con un promoter constitutive y un riboswitch el cuál inicia la traducción alrededor de los 32°C. Cómo se pretende producir el Vip3ca3 a temperaturas menores a lo 32°C, utilizamos un set de promotor y represor para invertir la activación de la producción de la proteína. Este modelo se puede utilizar como un regulador en la producción de sustancias contra plagas específicas; reduciría la sobreproducción, el efecto en terceros organismos, la bioacumulación y se podría cambiar el rango de temperaturas y/o la proteína para atacar a  otro tipo de plagas. </p>
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<p align="justify">Our project is about a genetic engineered machine in <i>E. coli</i> with the ability to produce Vip3ca3 which acts as a pesticide protein. Vip3ca3 production will be regulated by specific temperatures in order to avoid overproduction and it will show activity against target organisms Coleoptera and Lepidoptera, which are related to a local problem concerning potato crops. The Vip3ca3 production is regulated with a constitutive promoter and a riboswitch that initiate translation around 32°C. Since we want to produce the Vip3ca3 below the 32°C, we use a set of promoter-repressors in order to invert the activation of the protein production. This model may be used as a regulator in future transgenic plant generations for the production of substances against plagues, avoiding pesticide overproduction, thus reducing the effect in Non-Target Organisms and bioaccumulation and can be used with different temperature ranges and/or different proteins to attack other target organisms.</p>
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Latest revision as of 06:47, 22 June 2013

Project
Abstract

Thermonator III: The crop guardian

Our project is about a genetic engineered machine in E. coli with the ability to produce Vip3ca3 which acts as a pesticide protein. Vip3ca3 production will be regulated by specific temperatures in order to avoid overproduction and it will show activity against target organisms Coleoptera and Lepidoptera, which are related to a local problem concerning potato crops. The Vip3ca3 production is regulated with a constitutive promoter and a riboswitch that initiate translation around 32°C. Since we want to produce the Vip3ca3 below the 32°C, we use a set of promoter-repressors in order to invert the activation of the protein production. This model may be used as a regulator in future transgenic plant generations for the production of substances against plagues, avoiding pesticide overproduction, thus reducing the effect in Non-Target Organisms and bioaccumulation and can be used with different temperature ranges and/or different proteins to attack other target organisms.
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