Team:BV CAPS Kansas

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<span style="font-family:Helvetica;font-size:18px;font-weight:bold;text-decoration:none;text-transform:none;color:0174DF;">Welcome to CAPS </span>
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<b>Biofuels</b>
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The international oil industry drives our economy and very life of the world. However, this resource is dwindling and has detrimental effects on our atmosphere. In order to reduce oil usage and dependence, we will improve the efficiency of bio-fuels by manipulating the microbes used in their creation. One of the proposed biofuels is using alkanes to make biodiesel. They are useful because they float when the cell lysed and only needs to be dewatered. One way to produce alkanes is through Acetyl-CoA. Acetyl-CoA is synthesized by pyruvate. Pyruvate kinase converts phosphoenolpyruvate (PEP) into pyruvate. Pyruvate kinase adds a phosphate group from PEP to ADP to yield ATP and pyruvate. 
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Our iGEM project this year is to overexpress pyruvate kinase in cyanobacteria. To do this, we will insert a hyperactive pyruvate kinase from a rabbit muscle, yeast, or mutant cyanobacteria. This will increase the production of PEP greatly within the cyanobacteria. The excess PEP will then be used in the production of alkanes. Then, we will place the cyanobacteria in a low oxygen environment which will further suppress the usage of energy by limiting the electron transport chain. This energy would then be passed to the production of alkanes. Testing will be done with lipid extraction and profiling assay.
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!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=BV_CAPS_Kansas Official Team Profile]
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===Team===
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Blue Valley Center of Advanced Professional Studies (CAPS) is an outreach facility for students who wish to achieve higher goals and expectations in a professional environment. The team is composed of students from the Blue Valley district high schools giving us an array of students with different backgrounds and abilities.
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[https://2013hs.igem.org/Team:BV_CAPS_Kansas/Team Click here] to see our team members.
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[https://2013hs.igem.org/Team:BV_CAPS_Kansas/Notebook Click here] to see the monthly lab notebooks.
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===Results/Conclusions===
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What did you achieve over the course of your semester?
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What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
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[https://static.igem.org/mediawiki/2013hs/1/1c/CAPS_Bioscience_Lab_Safety_Manual.pdf] CAPS Bioscience Lab Safety Manual
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===Attributions===
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Who worked on what?
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[[File:Alec.jpg]]Alec Adams
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[[File:Corinne2.jpg]]Corinne Andresen
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[[File:Muriel.jpg]]Muriel Eaton
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[[File:Dylan.jpg]]Dylan Geick
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[[File:Austin.jpg]]Austin Jacobson
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[[File:Mason.jpg]]Mason Wilkinson
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Dr. Aron Fenton - [http://www.kumc.edu/school-of-medicine/biochemistry-and-molecular-biology/faculty/aron-w-fenton-phd.html]
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Eric Kessler
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Mr. Kessler’s interest in biology can be traced to his youth on his family’s annual canoe trips where he was obsessed with catching turtles.  He has earned three undergraduate degrees, in Education from UMKC, and Psychology and Zoology from the University of Texas. These studies were followed by a Masters in Biology from Emporia State University where he completed research on the retreat-site selection of woodland snakes. He complete 18 years at BVN before coming to CAPS, and maintains National Board Certification.
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Kelley Tuel
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Kelley Tuel will be the instructor for this course.  She is a MS Degree candidate in Biology at Emporia State University.  Her thesis is on the molecular genetics of Halobacterium sp. NRC-1, a salt-loving microbe, and its instructional use in high school classrooms.  For her research, she isolated and named a previously undescribed mutant strain of the bacteria possessing a novel gene that can copy and paste itself around the genome.  Colonies of the bacteria are in the adjacent image.  Kelley holds undergraduate degrees in Biology and Education from KSU, and has previously taught high school Biology and AP Biology.
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Joe Whalen
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Mr. Whalen began his adult life in biology as a commercial fisherman in Alaska.  His interest in the outdoors led him to complete an undergraduate degree in Environmental Science which he earned from the University of Kansas.  From there he worked as a liaison between research and high school education as a GK-12 Fellow, and then completed three years teaching Biology, Zoology, AP Biology, and some physical science at Blue Valley High School.  Before returning to CAPS he completed his Masters in Molecular and Cellular Biology at Oregon State University, where he studied genome defense mechanisms in the fungus, Fusarium graminearum.  Mr. Walen is currently responsible for teaching the Bioscience Research course.
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===Human Practices===
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What impact does/will your project have on the public?
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===Fun===
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Isn't Reading Quite Fun?
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'''References'''
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''General References on Biofuels''
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[http://cdn.intechopen.com/pdfs/43693/InTech-Metabolic_engineering_of_hydrocarbon_biosynthesis_for_biofuel_production.pdf] '''Metabolic Engineering of Hydrocarbon Biosynthesis for Biofuel Production''' by Anne M. Ruffing
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[http://onlinelibrary.wiley.com/doi/10.1002/biot.200900220/pdf] '''Advanced biofuel production in microbes''' by Pamela P. Peralta-Yahya1, and Jay D. Keasling
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[http://download.bioon.com.cn/upload/month_0906/20090602_9baf7ed8bf3db7b1eb2eXd9VaYDZvzAN.attach.pdf]  '''Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels''' by Sung Kuk Lee, Howard Chou, Timothy S Ham, Taek Soon Lee and Jay D Keasling
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[http://www.microbialcellfactories.com/content/pdf/1475-2859-9-3.pdf] '''The path to next generation biofuels: successes and challenges in the era of synthetic biology''' by Clementina Dellomonaco, Fabio Fava, Ramon Gonzalez
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[http://savagelab.org/media/papers/Savage_Silver_ACS_Chem_Biol_2008.pdf]  '''Defossiling Fuel: How Synthetic Biology Can Transform Biofuel Production''' by David F. Savage, Jeffrey Way, and Pamela A. Silver
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857869/pdf/JBB2010-761042.pdf]  '''Metabolic Engineering for Production of Biorenewable Fuels and Chemicals: Contributions of Synthetic Biology''' by Laura R. Jarboe, Xueli Zhang, Xuan Wang,Jonathan C. Moore,K. T. Shanmugam,and Lonnie O. Ingram
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2863401/pdf/zek486.pdf] '''Genetic Engineering of Algae for Enhanced Biofuel Production''' by Randor Radakovits, Robert E. Jinkerson, Al Darzins, and Matthew C. Posewitz
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[http://dspace.mit.edu/openaccess-disseminate/1721.1/68705] '''Synthetic Metabolism: Engineering Biology at the Protein and Pathway Scales''' by Collin H. Martin, David R. Nielsen, Kevin V. Solomon and Kristala L. Jones Prather
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[https://static.igem.org/mediawiki/2013hs/2/24/Jay_Keasling_etc....pdf]  '''Jay Keasling on Biofuels - a collection of links to references, videos, etc...'''
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''Cyanobacteria References''
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[http://pcp.oxfordjournals.org/content/44/7/758.full.pdf] '''From Genome to Enzyme: Analysis of Key Glycolytic and Oxidative PentosePhosphate Pathway Enzymes in the Cyanobacterium Synechocystis sp. PCC 6803''' by Vicki L. Knowles and William C. Plaxton
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[http://www.jbc.org/content/276/24/20966.full.pdf] '''Structural and Regulatory Properties of Pyruvate Kinase from the
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Cyanobacterium Synechococcus PCC 6301*''' by Vicki L. Knowles, Catherine S. Smith, Christopher R. Smith, and William C. Plaxton
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220531/pdf/10493919.pdf] '''Pathway alignment: application to the comparative analysis of glycolytic enzymes''' by Thomas Dandekar, Stefan Schuster‹, Berend Snel‹, Martijn Huynen‹Œ and Peer Bork
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[https://static.igem.org/mediawiki/2013hs/0/03/CO2-Limitation-Inducible_Green_Recovery_of_Fatty_Acids_from_Cyanobacteria_Biomass.pdf] '''CO2-limitation-inducible Green Recovery of fatty acids from cyanobacterial biomass''' by Xinyao Liu, Sarah Fallon, Jie Sheng, and Roy Curtiss III
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[http://www.pnas.org/content/110/19/7636.full.pdf] '''Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli''' by Thomas P. Howard, Sabine Middelhaufe, Karen Moore, Christoph Edner, Dagmara M. Kolak, George N. Taylor, David A. Parker, Rob Lee, Nicholas Smirnoff, Stephen J. Aves, and John Love
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''Synthetic Biology''
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[http://www.jbioleng.org/content/pdf/1754-1611-2-5.pdf] '''Engineering BioBrick vectors from BioBrick parts''' by Reshma P Shetty, Drew Endy and Thomas F Knight Jr
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[http://dspace.mit.edu/bitstream/handle/1721.1/21168/biobricks.pdf?sequence=1] '''Idempotent Vector Design for Standard Assembly of Biobricks''' by Tom Knight
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'''Diagrams'''
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[https://static.igem.org/mediawiki/2013hs/8/8c/Pathway_Diagram.PDF] Pathway Diagram
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[https://static.igem.org/mediawiki/2013hs/4/46/CyanoFuels_Fig.1.png] Another Pathway Diagram
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[https://static.igem.org/mediawiki/2013hs/c/cc/Image3.png] A 3rd Pathway Diagram
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'''Other Resources'''
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[http://www.tinkercell.com/] Tinker Cell Website
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[http://www.pdb.org/pdb/education_discussion/molecule_of_the_month/download/TheGlycoliticEnzymes.pdf] David Goodsell's piece on the Glycolytic Enzymes at the Protein Databank
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[https://igem.org/FAQ] iGEM FAQ, Wiki Editing
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background: -o-linear-gradient(top,  #3f3f3f 0%,#a0a0a0 100%); /* Opera 11.10+ */
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background: -ms-linear-gradient(top,  #3f3f3f 0%,#a0a0a0 100%); /* IE10+ */
 +
background: linear-gradient(to bottom,  #3f3f3f 0%,#a0a0a0 100%); /* W3C */
 +
filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#3f3f3f', endColorstr='#a0a0a0',GradientType=0 ); /* IE6-9 */
-
South Korean Team Slideshow tinkering
+
}
 +
.active-dot
 +
{
 +
background: #bfeefc; /* Old browsers */
 +
background: -moz-linear-gradient(top,  #bfeefc 0%, #0697db 100%); /* FF3.6+ */
 +
background: -webkit-gradient(linear, left top, left bottom, color-stop(0%,#bfeefc), color-stop(100%,#0697db)); /* Chrome,Safari4+ */
 +
background: -webkit-linear-gradient(top,  #bfeefc 0%,#0697db 100%); /* Chrome10+,Safari5.1+ */
 +
background: -o-linear-gradient(top,  #bfeefc 0%,#0697db 100%); /* Opera 11.10+ */
 +
background: -ms-linear-gradient(top,  #bfeefc 0%,#0697db 100%); /* IE10+ */
 +
background: linear-gradient(to bottom,  #bfeefc 0%,#0697db 100%); /* W3C */
 +
filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#bfeefc', endColorstr='#0697db',GradientType=0 ); /* IE6-9 */
-
<html>
+
}
-
<center>
+
</style>
<script type="text/javascript">
<script type="text/javascript">
-
var slideimages=new Array()
+
var jamboree;
-
function slideshowimages()
+
var countdown;
 +
countdownTimer=function()
{
{
-
    for (i=0;i<slideshowimages.arguments.length;i++){
+
var _loc6 = new Date();
-
        slideimages[i]=new Image()
+
var _loc2 = jamboree - _loc6;
-
        slideimages[i].src=slideshowimages.arguments[i]
+
if (_loc2 < 0){
-
    }
+
countdown.innerHTML="000:00:00:00";
 +
}else{
 +
var _loc1 = Math.floor(_loc2 / 86400000);
 +
var _loc4 = Math.floor(_loc2 / 3600000) % 24;
 +
var _loc5 = Math.floor(_loc2 / 60000) % 60;
 +
var _loc3 = Math.floor(_loc2 / 1000) % 60;
 +
if (_loc1 < 10){
 +
days = "00" + _loc1;
 +
}else if (_loc1 < 100){
 +
days = "0" + _loc1;
 +
}else{
 +
days = _loc1;
 +
}
 +
if (_loc4 < 10){
 +
hours = "0" + _loc4;
 +
}else{
 +
hours = _loc4;
 +
}
 +
if (_loc5 < 10){
 +
minutes = "0" + _loc5;
 +
}else{
 +
minutes = _loc5;
 +
}
 +
if (_loc3 < 10){
 +
seconds = "0" + _loc3;
 +
}else{
 +
seconds = _loc3;
 +
}
 +
countdown.innerHTML=days+":"+hours+":"+minutes+":"+seconds;
 +
}
 +
setTimeout(countdownTimer,500);
 +
}
 +
window.onload=function(){
 +
jamboree=new Date(2013,5,29,9);
 +
countdown=document.getElementById("countdown");
 +
countdownTimer();
}
}
</script>
</script>
-
 
+
<style type="text/css">
-
<img src="https://static.igem.org/mediawiki/2013hs/d/de/Landscape1.jpg" alt="Slideshow Image Script" title="Slideshow Image Script" name="slide" border=0 width=918 height=248><a href="http://hscripts.com" style="text-decoration:none;cursor:pointer;font-size:12px;color:green;"></a>
+
<!-- beginning of slider style -->
-
<script type="text/javascript">
+
#globalWrapper { background-color: transparent; border: none; margin: 0; padding: 0; width: 100%;
-
 
+
height:auto !important; /* real browsers */
-
slideshowimages("https://static.igem.org/mediawiki/2013hs/d/de/Landscape1.jpg","https://static.igem.org/mediawiki/2013hs/7/75/Landscape2.jpg","https://static.igem.org/mediawiki/2013hs/0/08/Landscape3.jpg","https://static.igem.org/mediawiki/2013hs/0/0d/Landscape4.jpg","https://static.igem.org/mediawiki/2013hs/9/98/Landscape5.jpg","https://static.igem.org/mediawiki/2013hs/6/6a/Landscape6.jpg","https://static.igem.org/mediawiki/2013hs/f/f5/Landscape7.jpg","https://static.igem.org/mediawiki/2013hs/c/c6/Landscape8.jpg")
+
height:100%; /* IE6: treaded as min-height*/
-
 
+
min-height:100%; /* real browsers */
-
var slideshowspeed=1500
+
}
-
var whichimage=0
+
#content { z-index: 1; background-color: transparent; border: none; padding: 0; margin: 0; width: 100%; overflow: hidden; margin-top: -45px !important;
-
function slideit()
+
height:auto !important; /* real browsers */
 +
height:100%; /* IE6: treaded as min-height*/
 +
min-height:100%; /* real browsers */
 +
}
 +
#bodyContent { border: none; padding:0; margin:0; width:100%;
 +
height:auto !important; /* real browsers */
 +
height:100%; /* IE6: treaded as min-height*/
 +
min-height:100%; /* real browsers */
 +
}
 +
#top-section { z-index: 2; height: 0px; margin: 0px; margin-left: auto; margin-right: auto; margin-bottom: 0 !important; padding:0; border: none; font-size: 10px;}
 +
#p-logo { height:1px; overflow:hidden; display: none;}
 +
#search-controls { overflow:hidden; display:block; background: none; position: absolute; top: 100px; right: 40px;}
 +
.left-menu { width: 500px !important; display:block; margin-top:-80px; border: none; text-align: right;}
 +
.left-menu ul { border: none; }
 +
#menubar.right-menu { width:300px; display:block; float:left; margin-top:-80px; border: none;}
 +
.right-menu ul { border: none; width: 300px;}
 +
#footer-box { background-color: #FFFF00; border: none; width: 850px; height: 30px; margin: -10px auto 0 auto; padding: 20px 0;}
 +
.visualClear { display: none; }
 +
#footer { border: none; width: 850px; margin: 0 auto; padding: 0;}
 +
.firstHeading { display: block;}
 +
#f-list a { color: #0019FF; font-size: 10px;}
 +
#f-list a:hover { color: #0019FF;}
 +
.printfooter { display: none; }
 +
#footer ul { margin: 0; padding: 0;}
 +
#footer ul li { margin-top: 0; margin-bottom: 0; margin-left: 10px; margin-right: 10px; padding: 0;}
 +
#search-controls { display:none; }
 +
h3#siteSub { display: none;}
 +
#contentSub {display: none;}
 +
p:first-child { display: block;}
 +
h1{border:none; width: 100%; clear: both;}
 +
<!-- end of slides style -->
 +
#globalWrapper {
 +
    position: relative;
 +
    font-size: 127%;
 +
    width: 850px //100%;
 +
    margin: 0;
 +
    padding: 0;
 +
    padding-bottom: 10px;
 +
#    background:url(https://static.igem.org/mediawiki/2013hs/5/5f/Cyano_Background2.jpg);
 +
#    background-repeat: no-repeat;
 +
#    background-attachment: fixed;
 +
#    background-size:100% auto;
 +
}
 +
#main-content {
 +
    position: relative;
 +
    width:  850px;
 +
    margin: 0 auto;
 +
    padding: 5px 5px 5px 5px;;
 +
    background: transparent;
 +
    color: black;
 +
#    border-left: 1px solid #ffffff;
 +
#    border-right: 1px solid #ffffff;
 +
#    line-height: 1.5em;
 +
}
 +
body {
 +
display:inherit;
 +
background-color: rgba(255,255,255,1);
 +
background-image: url('https://static.igem.org/mediawiki/2013hs/5/5f/Cyano_Background2.jpg');
 +
background-size: 100%;
 +
background-repeat: no-repeat;
 +
background-attachment:fixed;
 +
    color: black;
 +
    font: x-small sans-serif;
 +
    margin: 0;
 +
    padding: 0;
 +
#    height:100%;
 +
}
 +
#topmenubar {
 +
    position: relative;
 +
    white-space: nowrap;
 +
    top: -3px;
 +
    width: 850px;
 +
    z-index: 5;
 +
    font-family: sans-serif;
 +
    font-size: 80%;
 +
    line-height: 1em;
 +
}
 +
.topleft-menu, .topleft-menu a {
 +
    left: 0px;
 +
    text-align: right;
 +
    text-transform: lowercase;
 +
}
 +
.topleft-menu:hover {
 +
    color: blue;
 +
    background-color: rgba(174,181,184,0.7)
 +
}
 +
.topright-menu, .right-menu a {
 +
    right: 0px;
 +
    text-align: right;
 +
    color: blue;
 +
}
 +
#topmenubar ul {
 +
    color: #000000;
 +
    list-style: none;
 +
}
 +
#topmenubar li {
 +
    display: inline;
 +
    position: relative;
 +
    cursor: pointer;
 +
    padding-left: 0px;
 +
    padding-right: 0px;
 +
}
 +
.topleft-menu li a {
 +
    padding: 0px 7px 0px 0px;
 +
}
 +
.topleft-menu .selected {
 +
#    color: white;
 +
}
 +
#.left-menu .selected:hover {
 +
#    color: #0000AA;
 +
#}
 +
.topleft-menu:hover a {
 +
    color: #0000AA;
 +
}
 +
#newnavi {
 +
        position:relative;
 +
        height:42px;
 +
        width: 850px;
 +
        margin-left: -2px;
 +
        display:block;
 +
        background:transparent;
 +
}
 +
#newnavi .newmenu {
 +
        display:inline;
 +
        overflow:hidden;
 +
        padding:0px;
 +
        margin:2px solid transparent;
 +
        list-style-type:none;
 +
        height:24px;
 +
        width: 107px;
 +
        list-style-image:none;
 +
        background: #FFFFFF;
 +
}
 +
#newnavi .newmenu li    {
 +
        float:right;
 +
        background: #0000AA;
 +
        text-align:center;
 +
}
 +
#newnavi .newmenu .selected {
 +
        background: #000064;
 +
}
 +
#newnavi .newmenu li a  {
 +
text-align:center; vertical-align:middle; width:86px; padding:5px 10px 5px 10px; display:block; color:#FFFFFF; text-decoration:none; font:14px arial, verdana, sans-serif; font-weight: italic;
 +
}
 +
#newnavi .newmenu li:hover a {
 +
        text-decoration:none;
 +
}
 +
#newnavi .newmenu ul    {
 +
position:absolute; left:-9999px; top:-9999px; list-style-type:none; list-style-image:none; margin:0px; border:0px; padding:0px; background:  #63BC5B;
 +
}
 +
#newnavi .newmenu li:hover      {
 +
position:relative; background: #63BC5B;
 +
}
 +
#newnavi .newmenu li:hover ul  {
 +
left:0px; top:26px; background: #63BC5B;  width:150px; opacity:1.0 ; z-index:4;
 +
}
 +
#newnavi .newmenu li:hover ul li        {
 +
        background: #63BC5B;
 +
}
 +
#newnavi .newmenu li:hover ul li a      {
 +
text-align:left; padding:5px 0px 5px 0px;display:block; font-size:12px; width:150px; text-indent:10px; color:#FFFFFF;
 +
background-color: #0000AA; text-decoration:none;     
 +
}
 +
#newnavi .newmenu li:hover ul li a:hover {
 +
background: #63BC5B; color:#FFFFFF; width:150px;     
 +
}
 +
#newnavi .newmenu li ul li ul {
 +
        height:40px;
 +
        left:299px;
 +
        top:-26px;
 +
        margin: 0;
 +
padding: 0;
 +
list-style: none;
 +
width: 150px;
 +
        position:absolute;
 +
display:none;
 +
}
 +
#newnavi .newmenu li ul li ul li {
 +
position:relative;
 +
background: #000000;
 +
width: 150px;
 +
}
 +
#newnavi .newmenu li ul li ul li a {
 +
padding:5px 0px 5px 0px;display:block; font-size:12px; width:100px; text-indent:10px; color:#FFFFFF;
 +
background-color: #0019FF; text-decoration:none;
 +
}
 +
#newnavi .newmenu li ul li:hover ul {
 +
display:block;
 +
color:#FFFFFF;
 +
width:150px;
 +
background:transparent;
 +
}
 +
#newnavi .newmenu li ul li:hover ul li {
 +
top:-25px;
 +
left:150px;
 +
margin-bottom:-1px;
 +
height:26px;
 +
}
 +
#newnavi .newmenu li ul li:hover ul li a {
 +
padding:5px 0px 5px 0px;display:block; font-size:12px; text-indent:10px; color:#bbbbb;
 +
background-color: #0000AA; text-decoration:none;border:0px solid;
 +
}
 +
#slides {
 +
    position:relative;
 +
    height:280px;
 +
    width: 840px
 +
<!--change slide pic size here-->
 +
}
 +
#slides IMG {
 +
        position:absolute;
 +
        opacity:0.0;
 +
        top:0px;   
 +
        left:0px;
 +
        z-index:1;   
 +
}
 +
#slides .current {
 +
    z-index:3;
 +
    opacity:1.0;
 +
}
 +
#slides .previous {
 +
    z-index:2;
 +
}
 +
.progress {
 +
margin-top:-270px;
 +
margin-left:20px;
 +
position:absolute;
 +
}
 +
.progress li{
 +
border:1px solid rgba(0,0,0,0.9);
 +
border-radius:3px;
 +
margin-left: 5px;
 +
float:left;
 +
display:block;
 +
position:relative;
 +
z-index:10;
 +
background-color:rgba(102,200,255,0.4);
 +
  line-height:1.0em;
 +
}
 +
.progress li a{
 +
    padding-left: 6px;
 +
    padding-right: 6px;
 +
    font-size:12px;
 +
    line-height:1.0em;
 +
}
 +
.progress li a:hover {
 +
text-decoration:none;
 +
}
 +
.progress .current {
 +
background-color:#FFFFFF;
 +
}
 +
/* slide ends here */
 +
//leftbox, rightbox starts here
 +
#myleftrightbox
{
{
-
    if (!document.images)
+
position:relative;
-
          return
+
width:850px;
-
    document.images.slide.src=slideimages[whichimage].src
+
float:left;
-
    if (whichimage<slideimages.length-1)
+
background-color:#37FF00;
-
              whichimage++
+
margin-top: 0px;
-
    else
+
margin-left:3.75px solid transparent;
-
              whichimage=0
+
margin-right:3.75px solid transparent;
-
      setTimeout("slideit()",slideshowspeed)
+
border-radius: 4px;
 +
padding: 0px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: red;
}
}
-
  slideit()
+
#myleftrightbox .fourboxes
-
</script>
+
{
-
</html>
+
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: red;
 +
}
 +
#myleftrightbox .fourboxes-1
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftrightbox .fourboxes-2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftrightbox .fourboxes-3
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftrightbox .fourboxes-4
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftrightbox .fourboxes-1:hover {
 +
background-color:rgba(174,181,184,0.7);
 +
}
 +
#myleftrightbox .fourboxes-2:hover {
 +
background-color:rgba(174,181,184,0.7);
 +
}
 +
#myleftrightbox .fourboxes-3:hover {
 +
background-color:rgba(174,181,184,0.7);
 +
}
 +
#myleftrightbox .fourboxes-4:hover {
 +
background-color:rgba(174,181,184,0.7);
 +
}
 +
#myleftrightbox .fourboxes2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: red;
 +
}
 +
#myleftrightbox .fourboxes2-1
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: red;
 +
}
 +
#myleftrightbox .fourboxes2-2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftrightbox .fourboxes2-3
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
#myleftrightbox .fourboxes2-4
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
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 +
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 +
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#footer-box-1 {
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 +
        margin: 0 auto;
 +
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 +
<center>
 +
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#tweets .twtr-hd {
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    background: #d40;
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 +
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#tweets .twtr-hd h3,
 +
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 +
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 +
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#tweets .twtr-hd h3 {
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 +
}
 +
#tweets .twtr-tweet {
 +
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 +
    font: sans-serif !important;
 +
    font-size: 12px;
 +
}
 +
#tweets .twtr-tweet a:link,
 +
#tweets .twtr-tweet a:visited,
 +
#tweets .twtr-tweet a:hover {
 +
    color: #c40 !important;
 +
}
 +
#tweets .twtr-tweet .twtr-tweet-text {
 +
    border-bottom: 1px dashed #666 !important;
 +
    padding-bottom: 4px !important;
 +
    font: sans-serif;
 +
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 +
}
 +
#tweets .twtr-tweet .twtr-tweet-text p {
 +
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 +
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 +
    font-size: 12px;
 +
}
 +
//twitt style ends here
 +
</style>
 +
</head>
 +
</center>
 +
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_UC_Davis">
 +
  <div id="globalWrapper">
 +
  <div id="main-content">   
 +
<div id="topmenubar" class="topleft-menu" >
 +
  <ul>
 +
  <li style='color:#014457; cursor:default'><a>teams</a></li>
 +
                  <li class='selected'        ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas">Page</a></li>
 +
                <li class='new'><a href="https://2013hs.igem.org/wiki/index.php?title=Talk:Team:BV_CAPS_Kansas&amp;action=edit&amp;redlink=1">Discussion</a></li>
 +
              <li><a href="https://2013hs.igem.org/wiki/index.php?title=Team:BV_CAPS_Kansas&amp;action=edit">Edit</a></li>
 +
                          <li><a href="https://2013hs.igem.org/wiki/index.php?title=Team:BV_CAPS_Kansas&amp;action=history">History</a></li>
 +
                <li><a href="https://2013hs.igem.org/Special:MovePage/Team:BV_CAPS_Kansas">Move</a></li>
 +
                          <li><a href="https://2013hs.igem.org/wiki/index.php?title=Team:BV_CAPS_Kansas&amp;action=watch">Watch</a></li>
 +
  <li><a href="https://igem.org/Login">Log in</a></li>
 +
  </ul>
 +
</div>
 +
  <div id="newnavi">
 +
    <ul class="newmenu">
 +
    <li ><a target="new" href="https://2013.igem.org" title="Back to iGEM"><b>iGEM</b></a>
 +
          <ul>
 +
          <li><a target="new" href="https://2013.igem.org" >Main iGEM</a></li>
 +
          <li><a
 +
href="https://2013hs.igem.org" target="_blank">HS iGEM</a></li>
 +
          </ul>
 +
        </li>
 +
        <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Attributions"
 +
title="Attributions"><b>Attributions</b></a></li>
 +
        <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Outreach"
 +
title="Outreach"><b>Outreach</b></a></li>
 +
        <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Gallery"
 +
title="Fun"><b>Fun</b></a> <ul>
 +
            <li ><a title="Facebook">Facebook</a>
 +
                <ul>
 +
                  <li ><a href="https://www.facebook.com/groups/368737559905193/?fref=ts" target="_blank">HS iGEM</a></li>
 +
                  <li ><a href="https://www.facebook.com/capsigem2013?fref=ts" target="_blank">CAPS iGEM</a></li>
 +
                  <li ><a href="https://www.facebook.com/groups/igemmers/?fref=ts" target="_blank">iGEM</a></li>
 +
                </ul>
 +
              </li>
 +
            <li ><a title="Twitter">Twitter</a>
 +
                <ul>
 +
                  <li ><a href="https://twitter.com/CAPSiGEM" target="_blank">CAPS iGEM</a></li>
 +
                  <li ><a href="https://twitter.com/iGEM" target="_blank">iGEM HQ</a></li>
 +
                </ul>
 +
              </li>
 +
            <li ><a title="Slideshows">Slideshows</a>
 +
                <ul>
 +
                  <li ><a href="https://static.igem.org/mediawiki/2013hs/b/bd/CAPS_iGEM_Slideshow_2013.pdf" target="_blank">2013</a></li>
 +
                  <li ><a href="https://static.igem.org/mediawiki/2013hs/6/66/IGEM_2012_Slideshow.pdf" target="_blank">2012</a></li>
 +
                </ul>
 +
              </li>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Gallery">Glycolysis Poem</a></li>
 +
          </ul>
 +
        </li>
 +
              <ul>
 +
                <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Gallery">Glycolysis Poem</a></li>
 +
              </ul>
 +
            </li></li>
 +
        <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Safety"
 +
title="Safety"><b>Safety</b></a></li>
 +
        <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project" title="Project"><b>Project</b></a>
 +
          <ul>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project">Project Overview</a></li>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Problems">Problems</a></li>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Solutions">Solutions</a></li>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Notebook">Notebook</a></li>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Methods">Methods</a></li>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Achievements">Achievements</a></li>
 +
<li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/References">References</a></li>
 +
            <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Future">Future</a></li>
 +
          </ul>
 +
        </li>
 +
        <li ><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Team" title="Team"><b>Team</b></a></li>
 +
        <li  class="selected"><a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas" title="Home"><b>Home</b></a></li>
 +
    </ul>
 +
  </div>
 +
<!-- slide show starts here -->
 +
<div id="slides">
 +
    <img  src="https://static.igem.org/mediawiki/2013hs/d/d9/CyanoSchool.jpg" width="850" height="280" alt="" class="current" />
 +
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 +
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 +
    <img  src="https://static.igem.org/mediawiki/2013hs/b/bb/CyanoFun.jpg" width="850" height="280" alt="" />
 +
</div>
 +
    <ul class="progress">
 +
<li class="current"><a href="#n_0">1</a> </li>
 +
<li><a href="#n_1">2</a> </li>
 +
        <li><a href="#n_2">3</a> </li>
 +
        <li><a href="#n_3">4</a> </li>
 +
    </ul>
 +
<!-- slide show ends here -->
 +
<div id="bodyContent"> 
 +
<!--  <div id="contentSub"></div> -->
 +
<!--  </div> -->
 +
<!-- float: right; first try -->
 +
<!-- 1st row four boxes starts here -->
 +
<div id="myleftrightbox">
 +
<div id="myleftrightbox"  class="fourboxes-1">
 +
<a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Problems"><img src="https://static.igem.org/mediawiki/2013hs/f/f5/Problems_Brick.jpg
 +
"></a>
 +
</div>
 +
<div id="myleftrightbox"  class="spacebox">
 +
<p></p>
 +
</div>
 +
<div id="myleftrightbox"  class="fourboxes-2">
 +
<a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Solutions"><img src="https://static.igem.org/mediawiki/2013hs/1/10/SolutionBrick.jpg
 +
"></a>
 +
</div>
 +
<div id="myleftrightbox"  class="spacebox">
 +
<p></p>
 +
</div>
 +
<div id="myleftrightbox"  class="fourboxes-3">
 +
<a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Notebook"><img src="https://static.igem.org/mediawiki/2013hs/3/3e/Notebook_Brick.jpg"></a>
 +
</div>
 +
<div id="myleftrightbox"  class="spacebox">
 +
<p></p>
 +
</div>
 +
<div id="myleftrightbox"  class="fourboxes-4">
 +
<a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Methods"><img src="https://static.igem.org/mediawiki/2013hs/d/d6/Methods_Brick.jpg"></a>
 +
</div>
 +
</div>
 +
<!-- 1st row four boxes ends here -->
 +
<!-- 2nd row four boxes ends here -->
 +
<div id="myleftrightbox">
 +
<div id="myleftrightbox"  class="fourboxes2-1">
 +
<a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Achievements"><img src="https://static.igem.org/mediawiki/2013hs/5/50/Achievement_Brick.jpg"></a>
 +
</div>
 +
<div id="myleftrightbox"  class="spacebox">
 +
<p></p>
 +
</div>
 +
<div id="myleftrightbox"  class="fourboxes2-2">
 +
<a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Future"><img src="https://static.igem.org/mediawiki/2013hs/c/c1/Future_Brick.jpg"></a>
 +
</div>
 +
<div id="myleftrightbox"  class="spacebox">
 +
<p></p>
 +
</div>
 +
<div id="myleftrightbox"  class="fourboxes2-3">
 +
<a href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Gallery"><img src="https://static.igem.org/mediawiki/2013hs/0/0f/Fun_Button.jpg"></a>
 +
</div>
 +
<div id="myleftrightbox"  class="spacebox">
 +
<p></p>
 +
</div>
 +
<div id="myleftrightbox"  class="fourboxes2-4">
 +
<a href="https://2013hs.igem.org/Jamboree"><img src="https://static.igem.org/mediawiki/2013hs/9/9f/IGEM_Brick_Image.jpg"></a>
 +
</div>
 +
</div>
 +
<!-- 2nd row four boxes ends here -->
 +
<!-- <div id="sponsorbox" style="width:220px; float:right;"> -->
 +
<div id="sponsorbox">
 +
<div id="sponsorbox" class="sponsorfloat">
 +
<!-- twitter starts here -->
 +
</script>
 +
<div id="tweets">
 +
<center>
 +
<h2>BV CAPS iGEM Tweets</h2>
 +
</center>
 +
<a class="twitter-timeline" href="https://twitter.com/CAPSiGEM" data-widget-id="342763142138454016">Tweets by @CAPSiGEM</a>
 +
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<script charset="utf-8" src="http://widgets.twimg.com/j/2/widget.js"></script>
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<script>
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new TWTR.Widget({
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<h2>Thanks!</h2>
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<a href="https://www.microryza.com/projects/exploring-molecules-and-microbes" target="_blank"><img src="https://static.igem.org/mediawiki/2013hs/9/97/Microryzalarger.jpg" width="200"></a>
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<h1> Welcome </h1>
 +
<p>Welcome to the BV CAPS page!</p>
 +
This is the BV CAPS iGEM team’s second year participating in the iGEM jamboree. Our group is from Overland Park, Kansas where the state flower is the beautiful sunflower. Our program called Center for Advanced Professional Studies (CAPS) contains students from the five different high schools in the Blue Valley School District. At CAPS, students are able to learn in depth about a specific career path and are able to experience real-world problems. This year we are trying to overexpress pyruvate kinase in cyanobacteria to increase the production of alkanes which is one of the important biofuels.<br><br>
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<h1>iGEM 2013 High School Jamboree</h1>
 +
<div id="countdown">000:00:00:00</div>
 +
&nbsp; &nbsp; Days &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Hrs &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Min &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;  Sec&nbsp; &nbsp; </center><br>
 +
We can't wait to see you all at the competition!<br><br>
 +
Best viewed in Google Chrome &nbsp; (Download) &nbsp;<a href="https://www.google.com/intl/en/chrome/browser/?&brand=CHMB&utm_campaign=en&utm_source=en-ha-na-us-sk&utm_medium=ha" target="_blank"><img src="https://static.igem.org/mediawiki/2013hs/c/c1/Google_Chrome.png
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>
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style="text-indent:-15px;
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list-style-image:none;list-style-type:none;color:#000000;"><li><a
 +
style="color:#000000"
 +
href="https://2013hs.igem.org/Team:BV_CAPS_Kansas">Welcome</a> </li><li><a
 +
style="color:#000000"
 +
href="https://twitter.com/CAPSiGEM">Tweets</a></li><li><a
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style="color:#000000"
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style="color:#000000"
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project">Project Overview</a>
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</li><li><a style="color:#000000"
 +
href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Problems">Problems
 +
</a></li><li><a style="color:#000000"
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Solutions
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<li><a style="color:#000000"
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Notebook">Notebook
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</a></li>
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  <li><a style="color:#000000"
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Methods">Methods
 +
</a></li>
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  <li><a style="color:#000000"
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Achievements">Achievements</a></li>
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<li><a style="color:#000000"
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/References">References</a></li>
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<li><a style="color:#000000"
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Project/Future">Future</a>
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</li></ul> </li>
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<li style="float:left ;margin:0 10px"><a
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Safety "> <p>Safety</p></a>
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<a style="color:#000000 "
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href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Safety "> Safety</a> </li>
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"><li><a style="color:#000000 "
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 +
style="text-indent:-15px;list-style-image:none;list-style-type:none;color:#000000
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"></li><li><a style="color:#000000 "
 +
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Outreach</a> </li></ul>
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<li style="float:left ;margin:0 10px"><a
 +
href="https://2013hs.igem.org/Team:BV_CAPS_Kansas/Attributions ">
 +
<p>Attributions </p></a><a style="color:#000000 "
 +
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 +
Attributions</a></li>
 +
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 +
"><li><a style="color:#000000 " href="https://2013.igem.org/Main_Page
 +
">Main iGEM</a> </li><li><a style="color:#000000 "
 +
href="https://2013hs.igem.org"> HS iGEM</a>
 +
</li></ul>
 +
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 +
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Latest revision as of 18:59, 30 May 2014

Team:BV CAPS Kansas

BV CAPS iGEM Tweets

Thanks!

Welcome

Welcome to the BV CAPS page!

This is the BV CAPS iGEM team’s second year participating in the iGEM jamboree. Our group is from Overland Park, Kansas where the state flower is the beautiful sunflower. Our program called Center for Advanced Professional Studies (CAPS) contains students from the five different high schools in the Blue Valley School District. At CAPS, students are able to learn in depth about a specific career path and are able to experience real-world problems. This year we are trying to overexpress pyruvate kinase in cyanobacteria to increase the production of alkanes which is one of the important biofuels.

iGEM 2013 High School Jamboree

000:00:00:00
    Days                 Hrs               Min             Sec   

We can't wait to see you all at the competition!

Best viewed in Google Chrome   (Download)  

Retrieved from "https://2013hs.igem.org/Team:BV_CAPS_Kansas"

Retrieved from "http://2013hs.igem.org/Team:BV_CAPS_Kansas"