Team:CIDEB-UANL Mexico/Project-Circuit

From 2013hs.igem.org

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In summary: The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.<br>
In summary: The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.<br>
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For more details you can see the<a href="https://2013hs.igem.org/Team:CIDEB-UANL_Mexico/Project-Idea1 >Main Idea Description of the project.</a> </p>
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For more details you can see the <a href="https://2013hs.igem.org/Team:CIDEB-UANL_Mexico/Project-Idea1" >Main Idea Description of the project.</a> </p>
<p align="center"> <img src="https://static.igem.org/mediawiki/2013hs/3/37/Circuito2L.jpg" width="700px" height="300px" /> </p>
<p align="center"> <img src="https://static.igem.org/mediawiki/2013hs/3/37/Circuito2L.jpg" width="700px" height="300px" /> </p>

Revision as of 15:36, 20 June 2013

Project
Circuit

Circuit

The circuit works as the following way.
1.- A pR (repressible promoter) is active and produces the Vip3Ca3 protein and the Green Fluorescent Protein as a reporter of the Vip3Ca3 production.
2.- In the other part, there is a Constitutive promoter (pConst) but it works with a riboswitch.
3.- The riboswitch is activated after the 32ºC and it starts trasnlation producing the cI protein which inhibits the pR turning off the Vip3Ca3 production.
In summary: The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.
For more details you can see the Main Idea Description of the project.

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