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Team:CIDEB-UANL Mexico/Project-Circuit
From 2013hs.igem.org
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| - | <p><b>Circuit</b | + | <p><b>Circuit</b> |
<p align="justify">The circuit works as the following way. | <p align="justify">The circuit works as the following way. | ||
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| - | 1. | + | <b>1.</b> A pR (repressible promoter) is active and produces the Vip3Ca3 protein and the Green Fluorescent Protein as a reporter of the Vip3Ca3 production. |
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| - | 2. | + | <b>2.</b> In the other part, there is a Constitutive promoter (pConst) but it works with a riboswitch. |
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| - | 3. | + | <b>3.</b> The riboswitch is activated after the 32ºC and it starts trasnlation producing the cI protein which inhibits the pR turning off the Vip3Ca3 production. |
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| - | In summary: The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.<br> | + | <b>In summary:</b> The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.<br> |
| - | For more details you can see the <a href="https://2013hs.igem.org/Team:CIDEB-UANL_Mexico/Project-Idea1" >Main Idea Description of the project.</a | + | For more details you can see the circuit:<a href="https://2013hs.igem.org/Team:CIDEB-UANL_Mexico/Project-Idea1" >Main Idea Description of the project.</a> |
| - | < | + | <center><img src="https://static.igem.org/mediawiki/2013hs/3/37/Circuito2L.jpg" width="700px" height="300px" /></center> </p> |
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Revision as of 20:13, 20 June 2013
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Circuit
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Circuit The circuit works as the following way.
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CIDEB UANL Team. Centro de Investigación y Desarrollo de Educación Bilingüe |
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