Team:Lethbridge Canada/parts

From 2013hs.igem.org

(Difference between revisions)
Line 21: Line 21:
<a href="https://2013hs.igem.org/Team:Lethbridge_Canada">
<a href="https://2013hs.igem.org/Team:Lethbridge_Canada">
<div id="logo_all">
<div id="logo_all">
-
<img src="logo.png" alt="logo" />
+
<img src="https://static.igem.org/mediawiki/2013hs/3/32/Leth_hs_2013_logo.png" alt="logo" />
</div>
</div>
<span id="title_first">Lethbridge</span>
<span id="title_first">Lethbridge</span>

Revision as of 19:10, 6 June 2013

Parts

Parts:

Name Type Description Designer(s) Length
W BBa_J23100 Regulatory High constitutive promoter John Anderson 35
W BBa_J23108 Regulatory Medium-high constitutive promoter John Anderson 35
W BBa_J23113 Regulatory Medium-low constitutive promoter John Anderson 35
W BBa_J23114 Regulatory Low constitutive promoter John Anderson 35
W BBa_J61002 Plasmid_Backbone EX-Ptet-S-rbs-RFP-P “RFP reporter” John Anderson 2948
W BBa_B0034 RBS RBS (Elowitz 1999) defines RBS efficiency Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 12
W BBa_K314100 Composite J23100 promoter and B0034 RBS - High constitutive expression cassette Thomas Huber, Justin Siegel 518
W pSB1C3 Plasmid_Backbone High copy BioBrick assembly plasmid Austin Che 2070
Oxytocin-NeurophysinI_pUC57 Coding Protein hormone and carrier molecule for expression in pUC57 plasmid Lethbridge High School iGEM 406
NECI_pUC57 Coding Cleavage enzyme for expression in pUC57 plasmid Lethbridge High School iGEM 2277
BBa_J06702 Reporter mCherry, bacterial with RBS and forward terminator Yves Wang 869

Final Constructs:

Name Description Expanded Length Total Length
K314100+oxytocin-neurophysin1 in pSB1C3 BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin-neurophysin1 gene in pSB1C3 plasmid 518 + 406 + 2070 2994
K314100+NEC1 in pSB1C3 BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid 518 + 2277 + 2070 4865
J23100+J06702 in pSB1C3 BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23108+J06702 in pSB1C3 BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23113+J06702 in pSB1C3 BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23114+J06702 in pSB1C3 BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974