Team:Lethbridge Canada/parts

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Revision as of 20:08, 12 June 2013

Photo by: Missy S Link: http://www.flickr.com/photos/listenmissy/5087404401/ Photo by: Daniel Parks Link: http://www.flickr.com/photos/parksdh/5227623068/ Photo by: Mike Ranweiler Link: http://www.flickr.com/photos/27874907@N04/4833059991/ Photo by: Stuart SeegerLink: http://www.flickr.com/photos/stuseeger/97577796/

Parts:

Name Type Description Designer(s) Length
♥ ★ W BBa_J23100 Regulatory High constitutive promoter John Anderson 35
♥ ★ W BBa_J23108 Regulatory Medium-high constitutive promoter John Anderson 35
♥ ★ W BBa_J23113 Regulatory Medium-low constitutive promoter John Anderson 35
♥ ★ W BBa_J23114 Regulatory Low constitutive promoter John Anderson 35
♥ ★ W BBa_J61002 Plasmid_Backbone EX-Ptet-S-rbs-RFP-P “RFP reporter” John Anderson 2948
★ W BBa_B0034 RBS RBS (Elowitz 1999) defines RBS efficiency Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 12
♥ W BBa_K314100 Composite J23100 promoter and B0034 RBS - High constitutive expression cassette Thomas Huber, Justin Siegel 518
★ W pSB1C3 Plasmid_Backbone High copy BioBrick assembly plasmid Austin Che 2070
Oxytocin-NeurophysinI_pUC57 Coding Protein hormone and carrier molecule for expression in pUC57 plasmid Lethbridge High School iGEM 406
NECI_pUC57 Coding Cleavage enzyme for expression in pUC57 plasmid Lethbridge High School iGEM 2277
★ BBa_J06702 Reporter mCherry, bacterial with RBS and forward terminator Yves Wang 869

Final Constructs:

Name Description Expanded Length Total Length
K314100+oxytocin-neurophysin1 in pSB1C3 BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin-neurophysin1 gene in pSB1C3 plasmid 518 + 406 + 2070 2994
K314100+NEC1 in pSB1C3 BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid 518 + 2277 + 2070 4865
J23100+J06702 in pSB1C3 BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23108+J06702 in pSB1C3 BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23113+J06702 in pSB1C3 BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974
J23114+J06702 in pSB1C3 BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid 35 + 869 + 2070 2974

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