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- For our first plasmid, we would have BBa_I741005, the xylR transcriptional regular, which would start the production of the protein "xylR" which allows xylene to bind to our Pu promoter.
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Our second plasmid will be a DNA sequence consisting of:
- BBa_I723020 - 320 Bp - This is activated by the xylR transcriptional regulator in response to the detection of environmental xylene. When xylene in the presence of xylR, it binds to our promoter to start the sequence.
- BBa_B0030 - 15Bp - This is a strong RBS, which regulates the expression.
- BBa_E0040 - 720 Bp - This is our green fluorescent protein (wild type). It will work as the reporter for our sequence.
- BBa_B0015 - 129 Bp - This a double terminator, which is the stop codon for our sequence.
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- As a backbone for our plasmids, we will be using pSB1AK8, which has AmpR and KanR, as well as the killswitch BBa_P1010 incorporated into it.
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