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Circuit
The circuit works as the following way.
1. A pR (repressible promoter) is active and produces the Vip3Ca3 protein and the Green Fluorescent Protein as a reporter of the Vip3Ca3 production.
2. In the other part, there is a Constitutive promoter (pConst) but it works with a riboswitch.
3. The riboswitch is activated after the 32ºC and it starts trasnlation producing the cI protein which inhibits the pR turning off the Vip3Ca3 production.
In summary: The Vip3Ca3 production is regulated by temperature: it is produced below the 32ºC and at higher temperatures it is turned off.
For more details you can see the circuit:Main Idea Description of the project.
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