Team:CIDEB-UANL Mexico/WetLab-Notebook
From 2013hs.igem.org
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We prepared a digestion with the restriction enzymes with all the parts that we were going to need. This day it was done a replanting of the reporters GFP, CFP and YFP, and the plasmids 1-5A and 1-3A into LB medium and they were stored in the incubator. Also an electrophoresis was done with Friday’s cuts again, showing the same results. </p> | We prepared a digestion with the restriction enzymes with all the parts that we were going to need. This day it was done a replanting of the reporters GFP, CFP and YFP, and the plasmids 1-5A and 1-3A into LB medium and they were stored in the incubator. Also an electrophoresis was done with Friday’s cuts again, showing the same results. </p> | ||
<p align="justify"><b>June 11th, 2013</b><br> | <p align="justify"><b>June 11th, 2013</b><br> | ||
- | On this day, the colonies that were replanted yesterday doesn’t grow up, but even this we tried a MiniPrep in each sample. The results of the electrophoresis showed that only 3 DNA’s went well, while the other ones showed a faint DNA. Anyway, we decided to do the cuts with the enzymes of the 3 DNA’s that went well. Also this day, another replanting was done with the same samples of yesterday, to see if they grow correctly tomorrow. </p> | + | On this day, the colonies that were replanted yesterday doesn’t grow up, but even this we tried a MiniPrep in each sample. The results of the electrophoresis showed that only 3 DNA’s went well, while the other ones showed a faint DNA. Anyway, we decided to do the cuts with the enzymes of the 3 DNA’s that went well. Also this day, another replanting was done with the same samples of yesterday, to see if they grow correctly tomorrow. We ran a gel with only PCC1 and these were the results: </p> |
- | + | <center><img src="https://static.igem.org/mediawiki/2013hs/1/19/GeldegradadoD.png" height="300px" width="400px" /> | |
+ | <p>Image 3. Gel with only PCC1. First time it worked.<p></center> | ||
<p align="justify"><b>June 12th, 2013</b><br> | <p align="justify"><b>June 12th, 2013</b><br> | ||
The cuts that were done yesterday, were subject of an electrophoresis in agarose gel, the cuts showed the expected results except for the PCC1. It was decided to start all the MiniPreps again, to have a backup in case ligation would not function. This means a MiniPrep of all the reporter samples, GFP, YFP and CFP; the plasmids 1-3A and 1-5A; and the PUC, PCCI and 5-4E samples. After this, the results in the agarose gel show a very faint result with the DNA of the samples. | The cuts that were done yesterday, were subject of an electrophoresis in agarose gel, the cuts showed the expected results except for the PCC1. It was decided to start all the MiniPreps again, to have a backup in case ligation would not function. This means a MiniPrep of all the reporter samples, GFP, YFP and CFP; the plasmids 1-3A and 1-5A; and the PUC, PCCI and 5-4E samples. After this, the results in the agarose gel show a very faint result with the DNA of the samples. |
Revision as of 02:00, 22 June 2013
Wet-Lab
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Notebook
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May, 23th, 2013 May, 24th, 2013 May, 29th, 2013 May, 30th, 2013 May, 31th, 2013 June, 1st, 2013 June 3rd, 2013 June 4th, 2013 June 5th, 2013 June 6th, 2013 Image 1. Gel with the MiniPreps June 6th, 2013 June 7th, 2013 Image 2. Gel with the MiniPreps June 6th degradated June 10th, 2013 June 11th, 2013 Image 3. Gel with only PCC1. First time it worked. June 12th, 2013 June 13th, 2013 June 14th, 2013 June 17th, 2013 June 18th, 2013 June 20th, 2013 June 21th, 2013 |
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