Team:Lethbridge Canada/parts

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Lethbridge_Canada iGEM
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<p id="stmp">Parts</p>
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<a href="#place1" class="stmp">Preprooxyphysin</a>
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<a href="#place2" class="stmp">Protein Convertase I</a>
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<a href="#place3" class="stmp">Synthesis</a>
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Content 1
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<span id="title_first">Lethbridge</span>
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<span id="title_second">iGEM Team</span>
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Content 2
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<li class="navigation_project"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project" class="strict">Project</a>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Description</a></li>
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                                                <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project#video_oxy">Visual Modeling</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/math">Math Model</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/results">Results</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/wikifreeze">Wikifreeze</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols#protocol_header">Protocols</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">Notebook: March</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april">Notebook: April</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may">Notebook: May</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june">Notebook: June</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/presentations">Presentations</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/interviews">Interviews</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/surveys">Parent Surveys</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/novel_study">Novel Study</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_advisors">The Advisors</a></li>
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<th>Name</th>
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<th>Designer(s)</th>
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<th>Length</th>
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<td>&#9829;</td>
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<td>&#9733;</td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_J23100">BBa_J23100</a></td>
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<td>Regulatory</td>
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<td>High constitutive promoter</td>
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<td>John Anderson</td>
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<td>35</td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_J23108">BBa_J23108</a></td>
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<td>Regulatory</td>
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<p>
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<td>Medium-high constitutive promoter</td>
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Content 3
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<td>John Anderson</td>
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<td>35</td>
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<td>&#9829;</td>
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<td>&#9733;</td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_J23113">BBa_J23113</a></td>
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<td>Regulatory</td>
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<td>Medium-low constitutive promoter</td>
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<td>John Anderson</td>
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<td>35</td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_J23114">BBa_J23114</a></td>
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<td>Regulatory</td>
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<p>
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<td>Low constitutive promoter</td>
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Content 4
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<td>John Anderson</td>
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<td>35</td>
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<td>&#9829;</td>
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<td>&#9733;</td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_J61002">BBa_J61002</a></td>
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<td>Plasmid_Backbone</td>
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<td>EX-Ptet-S-rbs-RFP-P “RFP reporter”</td>
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<td>John Anderson</td>
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<td>2948</td>
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</tr>
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<td> </td>
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<td>&#9733;</td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_B0034">BBa_B0034</a></td>
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<td>RBS</td>
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<td>RBS (Elowitz 1999) defines RBS efficiency</td>
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<td>Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.</td>
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<td>12</td>
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<td>&#9829;</td>
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<td> </td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td>
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<td>Composite</td>
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<td>f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette</td>
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<td>Thomas Huber, Justin Siegel</td>
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<td>518</td>
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<td> </td>
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<td>&#9733;</td>
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<td>W</td>
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<td><a class="part" href="http://partsregistry.org/Part:pSB1C3">pSB1C3</a></td>
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<td>Plasmid_Backbone</td>
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<td>High copy BioBrick assembly plasmid</td>
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<td>Austin Che</td>
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<td>2070</td>
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<td> </td>
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<td> </td>
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<td> </td>
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<td>Oxytocin-Neurophysin I_pUC57</td>
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<td>Coding</td>
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<td>Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid</td>
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<td>Lethbridge High School iGEM</td>
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<td>406</td>
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</tr>
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<td> </td>
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<td> </td>
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<td> </td>
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<td>NECI_pUC57</td>
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<td>Coding</td>
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<td>Cleavage enzyme NECI gene in pUC57 plasmid</td>
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<td>Lethbridge High School iGEM</td>
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<td>2277</td>
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</tr>
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<tr>
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<td> </td>
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<td>&#9733;</td>
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<td> </td>
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<td><a class="part" href="http://partsregistry.org/Part:BBa_J06702">BBa_J06702</a></td>
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<td>Reporter</td>
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<td>mCherry, bacterial with RBS and forward terminator </td>
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<td>Yves Wang</td>
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<td>869</td>
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<td><a href="http://parts.igem.org/Part:BBa_K988003">K314100+oxytocin-neurophysin I in pSB1C3</a></td>
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<td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid</td>
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<td>518 + 406 + 2070</td>
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<td>2994</td>
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<td><a href="http://parts.igem.org/Part:BBa_K988002">Oxytocin-neurophysin I</a></td>
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<td>Oxytocin-neurophysin I gene in pSB1C3</td>
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<td>406</td>
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<td>406</td>
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</tr>
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    <td>K314100+NEC1 in pSB1C3</td>
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    <td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress)</td>
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    <td>518 + 2277 + 2070</td>
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    <td>4865</td>
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    </tr>
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    <tr>
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  <td>J23100+J06702 in pSB1C3</td>
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  <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
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  <td>35 + 869 + 2070</td>
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  <td>2974</td>
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</tr>
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    <td>J23108+J06702 in pSB1C3</td>
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    <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
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    <td>35 + 869 + 2070</td>
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    <td>2974</td>
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    </tr>
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    <td>J23113+J06702 in pSB1C3</td>
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    <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
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    <td>35 + 869 + 2070</td>
 +
    <td>2974</td>
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    </tr>
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 +
    <tr>
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    <td>J23114+J06702 in pSB1C3</td>
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    <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
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    <td>35 + 869 + 2070</td>
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    <td>2974</td>
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Latest revision as of 03:54, 22 June 2013

Parts

Name Type Description Designer(s) Length
♥ ★ W BBa_J23100 Regulatory High constitutive promoter John Anderson 35
♥ ★ W BBa_J23108 Regulatory Medium-high constitutive promoter John Anderson 35
♥ ★ W BBa_J23113 Regulatory Medium-low constitutive promoter John Anderson 35
♥ ★ W BBa_J23114 Regulatory Low constitutive promoter John Anderson 35
♥ ★ W BBa_J61002 Plasmid_Backbone EX-Ptet-S-rbs-RFP-P “RFP reporter” John Anderson 2948
★ W BBa_B0034 RBS RBS (Elowitz 1999) defines RBS efficiency Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 12
♥ W BBa_K314100 Composite f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette Thomas Huber, Justin Siegel 518
★ W pSB1C3 Plasmid_Backbone High copy BioBrick assembly plasmid Austin Che 2070
Oxytocin-Neurophysin I_pUC57 Coding Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid Lethbridge High School iGEM 406
NECI_pUC57 Coding Cleavage enzyme NECI gene in pUC57 plasmid Lethbridge High School iGEM 2277
★ BBa_J06702 Reporter mCherry, bacterial with RBS and forward terminator Yves Wang 869
Name Description Expanded Length Total Length
K314100+oxytocin-neurophysin I in pSB1C3 BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid 518 + 406 + 2070 2994
Oxytocin-neurophysin I Oxytocin-neurophysin I gene in pSB1C3 406 406
K314100+NEC1 in pSB1C3 BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress) 518 + 2277 + 2070 4865
J23100+J06702 in pSB1C3 BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23108+J06702 in pSB1C3 BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23113+J06702 in pSB1C3 BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23114+J06702 in pSB1C3 BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974

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