Team:Lethbridge Canada/parts

From 2013hs.igem.org

(Difference between revisions)
 
(31 intermediate revisions not shown)
Line 15: Line 15:
</head>
</head>
<body>
<body>
 +
<div id="parts">
<div id="parts">
<div id="wrapper">
<div id="wrapper">
Line 21: Line 22:
<a href="https://2013hs.igem.org/Team:Lethbridge_Canada">
<a href="https://2013hs.igem.org/Team:Lethbridge_Canada">
<div id="logo_all">
<div id="logo_all">
-
<img src="logo.png" alt="logo" />
+
<img src="https://static.igem.org/mediawiki/2013hs/3/32/Leth_hs_2013_logo.png" alt="logo" />
</div>
</div>
<span id="title_first">Lethbridge</span>
<span id="title_first">Lethbridge</span>
Line 28: Line 29:
</div>
</div>
-
<div id="content">
+
<div id="cont">
<div class="cleafix>">
<div class="cleafix>">
<div id="navigation">
<div id="navigation">
-
<ul>
+
<li class="navigation_project"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project" class="strict">Project</a>
-
<li class="navigation_project"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Project</a>
+
<ul>
<ul>
-
<li></li>
+
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Description</a></li>
 +
                                                <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project#video_oxy">Visual Modeling</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/math">Math Model</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/results">Results</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/wikifreeze">Wikifreeze</a></li>
</ul>
</ul>
</li>
</li>
-
<li class="navigation_parts"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/parts">Parts</a>
+
<li class="navigation_parts decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/parts" class="strict">Parts</a></li>
 +
<li class="navigation_notebook decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols" class="strict">Notebook</a>
<ul>
<ul>
-
<li></li>
+
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols#protocol_header">Protocols</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">Notebook: March</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april">Notebook: April</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may">Notebook: May</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june">Notebook: June</a></li>
</ul>
</ul>
</li>
</li>
-
<li class="navigation_notebook"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols">Notebook</a></li>
+
<li class="navigation_safety decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/safety" class="strict">Safety</a></li>
-
<li class="navigation_safety"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/safety">Safety</a></li>
+
<li class="navigation_outreach decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach" class="strict">Outreach</a>
-
<li class="navigation_outreach"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach">Outreach</a></li>
+
<ul>
-
<li class="navigation_team"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team">Team</a></li>
+
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach">Overview</a></li>
-
<li class="navigation_sponsors"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/sponsors">Sponsors</a></li>
+
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/presentations">Presentations</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/interviews">Interviews</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/videos">Videos</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/surveys">Parent Surveys</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/novel_study">Novel Study</a></li>
 +
</ul>
 +
</li>
 +
<li class="navigation_team decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team" class="strict">Team</a>
 +
<ul>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_team">The Team</a></li>
 +
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_advisors">The Advisors</a></li>
 +
</ul>
 +
</li>
 +
<li class="navigation_sponsors decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/sponsors" class="strict">Sponsors</a></li>
</ul>
</ul>
</div>
</div>
-
</div>
+
</div>
-
+
 
-
+
<div id="mainContent">
-
<div id="mainContent">
+
-
+
<div id="parts_section">
<div id="parts_section">
 
 
-
  <h2 class="table_title">Parts:</h2>
+
  <img class="parts_banner_image" src="https://static.igem.org/mediawiki/2013hs/a/a2/Lethbridge_hs_igem_2013_banner_parts.png">
    
    
  <table class="sortable" id="sorter">
  <table class="sortable" id="sorter">
Line 144: Line 165:
<td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td>
<td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td>
<td>Composite</td>
<td>Composite</td>
-
<td>J23100 promoter and B0034 RBS - High constitutive expression cassette</td>
+
<td>f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette</td>
<td>Thomas Huber, Justin Siegel</td>
<td>Thomas Huber, Justin Siegel</td>
<td>518</td>
<td>518</td>
Line 164: Line 185:
<td> </td>
<td> </td>
<td> </td>
<td> </td>
-
<td>Oxytocin-NeurophysinI_pUC57</td>
+
<td>Oxytocin-Neurophysin I_pUC57</td>
<td>Coding</td>
<td>Coding</td>
-
<td>Protein hormone and carrier molecule for expression in pUC57 plasmid</td>
+
<td>Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid</td>
<td>Lethbridge High School iGEM</td>
<td>Lethbridge High School iGEM</td>
<td>406</td>
<td>406</td>
Line 177: Line 198:
<td>NECI_pUC57</td>
<td>NECI_pUC57</td>
<td>Coding</td>
<td>Coding</td>
-
<td>Cleavage enzyme for expression in pUC57 plasmid</td>
+
<td>Cleavage enzyme NECI gene in pUC57 plasmid</td>
<td>Lethbridge High School iGEM</td>
<td>Lethbridge High School iGEM</td>
<td>2277</td>
<td>2277</td>
Line 203: Line 224:
<div id="constructs_section">
<div id="constructs_section">
-
<h2 class="table_title">Final Constructs:</h2>
+
<img class="parts_banner_image" src="https://static.igem.org/mediawiki/2013hs/a/a6/Lethbridge_hs_igem_2013_banner_finalcontructs.png">
    
    
  <table class="sortable" id="sorter2">
  <table class="sortable" id="sorter2">
Line 210: Line 231:
    <th>Name</th>
    <th>Name</th>
    <th>Description</th>
    <th>Description</th>
-
    <th class="nosort">Expanded Length</th>
+
    <th>Expanded Length</th>
    <th>Total Length</th>
    <th>Total Length</th>
  </tr>
  </tr>
     
     
  <tr>
  <tr>
-
<td>K314100+oxytocin-neurophysin1 in pSB1C3</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K988003">K314100+oxytocin-neurophysin I in pSB1C3</a></td>
-
<td>BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin-neurophysin1 gene in pSB1C3 plasmid</td>
+
<td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid</td>
<td>518 + 406 + 2070</td>
<td>518 + 406 + 2070</td>
<td>2994</td>
<td>2994</td>
 +
</tr>
 +
 +
  <tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K988002">Oxytocin-neurophysin I</a></td>
 +
<td>Oxytocin-neurophysin I gene in pSB1C3</td>
 +
<td>406</td>
 +
<td>406</td>
</tr>
</tr>
     
     
<tr>
<tr>
    <td>K314100+NEC1 in pSB1C3</td>
    <td>K314100+NEC1 in pSB1C3</td>
-
    <td>BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid</td>
+
    <td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress)</td>
    <td>518 + 2277 + 2070</td>
    <td>518 + 2277 + 2070</td>
    <td>4865</td>
    <td>4865</td>
Line 230: Line 258:
    <tr>
    <tr>
  <td>J23100+J06702 in pSB1C3</td>
  <td>J23100+J06702 in pSB1C3</td>
-
  <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
+
  <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
  <td>35 + 869 + 2070</td>
  <td>35 + 869 + 2070</td>
  <td>2974</td>
  <td>2974</td>
Line 237: Line 265:
<tr>
<tr>
    <td>J23108+J06702 in pSB1C3</td>
    <td>J23108+J06702 in pSB1C3</td>
-
    <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
+
    <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
    <td>35 + 869 + 2070</td>
    <td>35 + 869 + 2070</td>
    <td>2974</td>
    <td>2974</td>
Line 244: Line 272:
    <tr>
    <tr>
    <td>J23113+J06702 in pSB1C3</td>
    <td>J23113+J06702 in pSB1C3</td>
-
    <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
+
    <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
    <td>35 + 869 + 2070</td>
    <td>35 + 869 + 2070</td>
    <td>2974</td>
    <td>2974</td>
Line 251: Line 279:
    <tr>
    <tr>
    <td>J23114+J06702 in pSB1C3</td>
    <td>J23114+J06702 in pSB1C3</td>
-
    <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
+
    <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
    <td>35 + 869 + 2070</td>
    <td>35 + 869 + 2070</td>
    <td>2974</td>
    <td>2974</td>

Latest revision as of 03:54, 22 June 2013

Parts

Name Type Description Designer(s) Length
♥ ★ W BBa_J23100 Regulatory High constitutive promoter John Anderson 35
♥ ★ W BBa_J23108 Regulatory Medium-high constitutive promoter John Anderson 35
♥ ★ W BBa_J23113 Regulatory Medium-low constitutive promoter John Anderson 35
♥ ★ W BBa_J23114 Regulatory Low constitutive promoter John Anderson 35
♥ ★ W BBa_J61002 Plasmid_Backbone EX-Ptet-S-rbs-RFP-P “RFP reporter” John Anderson 2948
★ W BBa_B0034 RBS RBS (Elowitz 1999) defines RBS efficiency Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 12
♥ W BBa_K314100 Composite f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette Thomas Huber, Justin Siegel 518
★ W pSB1C3 Plasmid_Backbone High copy BioBrick assembly plasmid Austin Che 2070
Oxytocin-Neurophysin I_pUC57 Coding Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid Lethbridge High School iGEM 406
NECI_pUC57 Coding Cleavage enzyme NECI gene in pUC57 plasmid Lethbridge High School iGEM 2277
★ BBa_J06702 Reporter mCherry, bacterial with RBS and forward terminator Yves Wang 869
Name Description Expanded Length Total Length
K314100+oxytocin-neurophysin I in pSB1C3 BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid 518 + 406 + 2070 2994
Oxytocin-neurophysin I Oxytocin-neurophysin I gene in pSB1C3 406 406
K314100+NEC1 in pSB1C3 BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress) 518 + 2277 + 2070 4865
J23100+J06702 in pSB1C3 BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23108+J06702 in pSB1C3 BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23113+J06702 in pSB1C3 BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23114+J06702 in pSB1C3 BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974

Retrieved from "http://2013hs.igem.org/Team:Lethbridge_Canada/parts"