Team:Lethbridge Canada/parts

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<div id="parts_section">
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  <h2 class="table_title">Parts:</h2>
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  <img class="parts_banner_image" src="https://static.igem.org/mediawiki/2013hs/a/a2/Lethbridge_hs_igem_2013_banner_parts.png">
    
    
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<td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td>
<td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td>
<td>Composite</td>
<td>Composite</td>
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<td>J23100 promoter and B0034 RBS - High constitutive expression cassette</td>
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<td>f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette</td>
<td>Thomas Huber, Justin Siegel</td>
<td>Thomas Huber, Justin Siegel</td>
<td>518</td>
<td>518</td>
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<td> </td>
<td> </td>
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<td>Oxytocin-NeurophysinI_pUC57</td>
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<td>Oxytocin-Neurophysin I_pUC57</td>
<td>Coding</td>
<td>Coding</td>
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<td>Protein hormone and carrier molecule for expression in pUC57 plasmid</td>
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<td>Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid</td>
<td>Lethbridge High School iGEM</td>
<td>Lethbridge High School iGEM</td>
<td>406</td>
<td>406</td>
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<td>NECI_pUC57</td>
<td>NECI_pUC57</td>
<td>Coding</td>
<td>Coding</td>
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<td>Cleavage enzyme for expression in pUC57 plasmid</td>
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<td>Cleavage enzyme NECI gene in pUC57 plasmid</td>
<td>Lethbridge High School iGEM</td>
<td>Lethbridge High School iGEM</td>
<td>2277</td>
<td>2277</td>
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<div id="constructs_section">
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<h2 class="table_title">Final Constructs:</h2>
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<td>K314100+oxytocin-neurophysin1 in pSB1C3</td>
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<td><a href="http://parts.igem.org/Part:BBa_K988003">K314100+oxytocin-neurophysin I in pSB1C3</a></td>
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<td>BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin-neurophysin1 gene in pSB1C3 plasmid</td>
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<td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid</td>
<td>518 + 406 + 2070</td>
<td>518 + 406 + 2070</td>
<td>2994</td>
<td>2994</td>
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</tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K988002">Oxytocin-neurophysin I</a></td>
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<td>Oxytocin-neurophysin I gene in pSB1C3</td>
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<td>406</td>
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<td>406</td>
</tr>
</tr>
     
     
<tr>
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    <td>K314100+NEC1 in pSB1C3</td>
    <td>K314100+NEC1 in pSB1C3</td>
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    <td>BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid</td>
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    <td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress)</td>
    <td>518 + 2277 + 2070</td>
    <td>518 + 2277 + 2070</td>
    <td>4865</td>
    <td>4865</td>
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    <tr>
    <tr>
  <td>J23100+J06702 in pSB1C3</td>
  <td>J23100+J06702 in pSB1C3</td>
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  <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
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  <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
  <td>35 + 869 + 2070</td>
  <td>35 + 869 + 2070</td>
  <td>2974</td>
  <td>2974</td>
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<tr>
<tr>
    <td>J23108+J06702 in pSB1C3</td>
    <td>J23108+J06702 in pSB1C3</td>
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    <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
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    <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
    <td>35 + 869 + 2070</td>
    <td>35 + 869 + 2070</td>
    <td>2974</td>
    <td>2974</td>
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    <tr>
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    <td>J23113+J06702 in pSB1C3</td>
    <td>J23113+J06702 in pSB1C3</td>
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    <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
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    <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
    <td>35 + 869 + 2070</td>
    <td>35 + 869 + 2070</td>
    <td>2974</td>
    <td>2974</td>
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    <td>J23114+J06702 in pSB1C3</td>
    <td>J23114+J06702 in pSB1C3</td>
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    <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td>
+
    <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td>
    <td>35 + 869 + 2070</td>
    <td>35 + 869 + 2070</td>
    <td>2974</td>
    <td>2974</td>

Latest revision as of 03:54, 22 June 2013

Parts

Name Type Description Designer(s) Length
♥ ★ W BBa_J23100 Regulatory High constitutive promoter John Anderson 35
♥ ★ W BBa_J23108 Regulatory Medium-high constitutive promoter John Anderson 35
♥ ★ W BBa_J23113 Regulatory Medium-low constitutive promoter John Anderson 35
♥ ★ W BBa_J23114 Regulatory Low constitutive promoter John Anderson 35
♥ ★ W BBa_J61002 Plasmid_Backbone EX-Ptet-S-rbs-RFP-P “RFP reporter” John Anderson 2948
★ W BBa_B0034 RBS RBS (Elowitz 1999) defines RBS efficiency Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. 12
♥ W BBa_K314100 Composite f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette Thomas Huber, Justin Siegel 518
★ W pSB1C3 Plasmid_Backbone High copy BioBrick assembly plasmid Austin Che 2070
Oxytocin-Neurophysin I_pUC57 Coding Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid Lethbridge High School iGEM 406
NECI_pUC57 Coding Cleavage enzyme NECI gene in pUC57 plasmid Lethbridge High School iGEM 2277
★ BBa_J06702 Reporter mCherry, bacterial with RBS and forward terminator Yves Wang 869
Name Description Expanded Length Total Length
K314100+oxytocin-neurophysin I in pSB1C3 BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid 518 + 406 + 2070 2994
Oxytocin-neurophysin I Oxytocin-neurophysin I gene in pSB1C3 406 406
K314100+NEC1 in pSB1C3 BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress) 518 + 2277 + 2070 4865
J23100+J06702 in pSB1C3 BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23108+J06702 in pSB1C3 BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23113+J06702 in pSB1C3 BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974
J23114+J06702 in pSB1C3 BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) 35 + 869 + 2070 2974

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