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Team:Lethbridge Canada/parts
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<div id="mainContent"> | <div id="mainContent"> | ||
| - | + | ||
<div id="parts_section"> | <div id="parts_section"> | ||
| - | < | + | <img class="parts_banner_image" src="https://static.igem.org/mediawiki/2013hs/a/a2/Lethbridge_hs_igem_2013_banner_parts.png"> |
<table class="sortable" id="sorter"> | <table class="sortable" id="sorter"> | ||
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<td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td> | <td><a class="part" href="http://partsregistry.org/Part:BBa_K314100">BBa_K314100</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
| - | <td>J23100 promoter and B0034 RBS - High constitutive expression cassette</td> | + | <td>f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette</td> |
<td>Thomas Huber, Justin Siegel</td> | <td>Thomas Huber, Justin Siegel</td> | ||
<td>518</td> | <td>518</td> | ||
| Line 165: | Line 185: | ||
<td> </td> | <td> </td> | ||
<td> </td> | <td> </td> | ||
| - | <td>Oxytocin- | + | <td>Oxytocin-Neurophysin I_pUC57</td> |
<td>Coding</td> | <td>Coding</td> | ||
| - | <td> | + | <td>Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid</td> |
<td>Lethbridge High School iGEM</td> | <td>Lethbridge High School iGEM</td> | ||
<td>406</td> | <td>406</td> | ||
| Line 178: | Line 198: | ||
<td>NECI_pUC57</td> | <td>NECI_pUC57</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| - | <td>Cleavage enzyme | + | <td>Cleavage enzyme NECI gene in pUC57 plasmid</td> |
<td>Lethbridge High School iGEM</td> | <td>Lethbridge High School iGEM</td> | ||
<td>2277</td> | <td>2277</td> | ||
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<div id="constructs_section"> | <div id="constructs_section"> | ||
| - | < | + | <img class="parts_banner_image" src="https://static.igem.org/mediawiki/2013hs/a/a6/Lethbridge_hs_igem_2013_banner_finalcontructs.png"> |
<table class="sortable" id="sorter2"> | <table class="sortable" id="sorter2"> | ||
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<tr> | <tr> | ||
| - | <td>K314100+oxytocin- | + | <td><a href="http://parts.igem.org/Part:BBa_K988003">K314100+oxytocin-neurophysin I in pSB1C3</a></td> |
| - | <td>BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin- | + | <td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid</td> |
<td>518 + 406 + 2070</td> | <td>518 + 406 + 2070</td> | ||
<td>2994</td> | <td>2994</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td><a href="http://parts.igem.org/Part:BBa_K988002">Oxytocin-neurophysin I</a></td> | ||
| + | <td>Oxytocin-neurophysin I gene in pSB1C3</td> | ||
| + | <td>406</td> | ||
| + | <td>406</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>K314100+NEC1 in pSB1C3</td> | <td>K314100+NEC1 in pSB1C3</td> | ||
| - | <td>BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid</td> | + | <td>BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress)</td> |
<td>518 + 2277 + 2070</td> | <td>518 + 2277 + 2070</td> | ||
<td>4865</td> | <td>4865</td> | ||
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<tr> | <tr> | ||
<td>J23100+J06702 in pSB1C3</td> | <td>J23100+J06702 in pSB1C3</td> | ||
| - | <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td> | + | <td>BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td> |
<td>35 + 869 + 2070</td> | <td>35 + 869 + 2070</td> | ||
<td>2974</td> | <td>2974</td> | ||
| Line 238: | Line 265: | ||
<tr> | <tr> | ||
<td>J23108+J06702 in pSB1C3</td> | <td>J23108+J06702 in pSB1C3</td> | ||
| - | <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td> | + | <td>BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td> |
<td>35 + 869 + 2070</td> | <td>35 + 869 + 2070</td> | ||
<td>2974</td> | <td>2974</td> | ||
| Line 245: | Line 272: | ||
<tr> | <tr> | ||
<td>J23113+J06702 in pSB1C3</td> | <td>J23113+J06702 in pSB1C3</td> | ||
| - | <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td> | + | <td>BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td> |
<td>35 + 869 + 2070</td> | <td>35 + 869 + 2070</td> | ||
<td>2974</td> | <td>2974</td> | ||
| Line 252: | Line 279: | ||
<tr> | <tr> | ||
<td>J23114+J06702 in pSB1C3</td> | <td>J23114+J06702 in pSB1C3</td> | ||
| - | <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid</td> | + | <td>BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress)</td> |
<td>35 + 869 + 2070</td> | <td>35 + 869 + 2070</td> | ||
<td>2974</td> | <td>2974</td> | ||
Latest revision as of 03:54, 22 June 2013
| Name | Type | Description | Designer(s) | Length | |||
|---|---|---|---|---|---|---|---|
| ♥ | ★ | W | BBa_J23100 | Regulatory | High constitutive promoter | John Anderson | 35 |
| ♥ | ★ | W | BBa_J23108 | Regulatory | Medium-high constitutive promoter | John Anderson | 35 |
| ♥ | ★ | W | BBa_J23113 | Regulatory | Medium-low constitutive promoter | John Anderson | 35 |
| ♥ | ★ | W | BBa_J23114 | Regulatory | Low constitutive promoter | John Anderson | 35 |
| ♥ | ★ | W | BBa_J61002 | Plasmid_Backbone | EX-Ptet-S-rbs-RFP-P “RFP reporter” | John Anderson | 2948 |
| ★ | W | BBa_B0034 | RBS | RBS (Elowitz 1999) defines RBS efficiency | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 12 | |
| ♥ | W | BBa_K314100 | Composite | f1 phage origin, J23100 promoter and B0034 RBS - High constitutive expression cassette | Thomas Huber, Justin Siegel | 518 | |
| ★ | W | pSB1C3 | Plasmid_Backbone | High copy BioBrick assembly plasmid | Austin Che | 2070 | |
| Oxytocin-Neurophysin I_pUC57 | Coding | Oxytocin hormone and carrier protein Neurophysin I genes in pUC57 plasmid | Lethbridge High School iGEM | 406 | |||
| NECI_pUC57 | Coding | Cleavage enzyme NECI gene in pUC57 plasmid | Lethbridge High School iGEM | 2277 | |||
| ★ | BBa_J06702 | Reporter | mCherry, bacterial with RBS and forward terminator | Yves Wang | 869 |
| Name | Description | Expanded Length | Total Length |
|---|---|---|---|
| K314100+oxytocin-neurophysin I in pSB1C3 | BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and oxytocin-neurophysin I gene in pSB1C3 plasmid | 518 + 406 + 2070 | 2994 |
| Oxytocin-neurophysin I | Oxytocin-neurophysin I gene in pSB1C3 | 406 | 406 |
| K314100+NEC1 in pSB1C3 | BBa_K314100 (f1 phage origin, J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid (in progress) | 518 + 2277 + 2070 | 4865 |
| J23100+J06702 in pSB1C3 | BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) | 35 + 869 + 2070 | 2974 |
| J23108+J06702 in pSB1C3 | BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) | 35 + 869 + 2070 | 2974 |
| J23113+J06702 in pSB1C3 | BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) | 35 + 869 + 2070 | 2974 |
| J23114+J06702 in pSB1C3 | BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid (in progress) | 35 + 869 + 2070 | 2974 |
