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Team:Lethbridge Canada/results
From 2013hs.igem.org
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<p>To detect our preprooxyphysin proteins we did a Silver Stain. The reason for doing a Silver Stain is that it is 10x more sensitive than the standard Coomassie stain. In Lane 2, we did a control of Cbf5-Nop10-Gar1. This is actually three proteins. Because of their re;latively larger size, Cbf5 and Nop10 are found at the top of Lane 2. However, Gar1 is smaller, only 7kDa, and is found to be at the bottom. It is outlined in red. Preprooxyphysin is roughly twice as large as Gar1 at 13kDa and is found to about twice as large as Gar1. It is outlined by orange.</p> | <p>To detect our preprooxyphysin proteins we did a Silver Stain. The reason for doing a Silver Stain is that it is 10x more sensitive than the standard Coomassie stain. In Lane 2, we did a control of Cbf5-Nop10-Gar1. This is actually three proteins. Because of their re;latively larger size, Cbf5 and Nop10 are found at the top of Lane 2. However, Gar1 is smaller, only 7kDa, and is found to be at the bottom. It is outlined in red. Preprooxyphysin is roughly twice as large as Gar1 at 13kDa and is found to about twice as large as Gar1. It is outlined by orange.</p> | ||
| - | <p>We ran a 16.5% tris-tricine PAGE which | + | <p>We ran a 16.5% tris-tricine PAGE which separates better than than an SDS PAGE. An tris-tricine PAGE contains tricine. Tricine is more effective at separating proteins than the glycine of an SDS PAGE.</p> |
</div> | </div> | ||
Revision as of 03:38, 21 June 2013
| Lane | Contents | Volume |
|---|---|---|
| 1 | Protein Molecular Weight Marker | 20μL |
| 2 | Cbf5-Nop10-Gar1 | 20μL |
| 3 | Buffer A wash | 30μL |
| 4 | Buffer B wash | 30μL |
| 5 | Buffer E Wash | 30μL |
| 6 | S-30 s/N | 30μL |
| 7 | Buffer E – Concentrated Preprooxyphysin | 30μL |
To detect our preprooxyphysin proteins we did a Silver Stain. The reason for doing a Silver Stain is that it is 10x more sensitive than the standard Coomassie stain. In Lane 2, we did a control of Cbf5-Nop10-Gar1. This is actually three proteins. Because of their re;latively larger size, Cbf5 and Nop10 are found at the top of Lane 2. However, Gar1 is smaller, only 7kDa, and is found to be at the bottom. It is outlined in red. Preprooxyphysin is roughly twice as large as Gar1 at 13kDa and is found to about twice as large as Gar1. It is outlined by orange.
We ran a 16.5% tris-tricine PAGE which separates better than than an SDS PAGE. An tris-tricine PAGE contains tricine. Tricine is more effective at separating proteins than the glycine of an SDS PAGE.
