Parts:

			Name	Type	Description	Designer(s)	Length
♥	★	W	BBa_J23100	Regulatory	High constitutive promoter	John Anderson	35
♥	★	W	BBa_J23108	Regulatory	Medium-high constitutive promoter	John Anderson	35
♥	★	W	BBa_J23113	Regulatory	Medium-low constitutive promoter	John Anderson	35
♥	★	W	BBa_J23114	Regulatory	Low constitutive promoter	John Anderson	35
♥	★	W	BBa_J61002	Plasmid_Backbone	EX-Ptet-S-rbs-RFP-P “RFP reporter”	John Anderson	2948
	★	W	BBa_B0034	RBS	RBS (Elowitz 1999) defines RBS efficiency	Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.	12
♥		W	BBa_K314100	Composite	J23100 promoter and B0034 RBS - High constitutive expression cassette	Thomas Huber, Justin Siegel	518
	★	W	pSB1C3	Plasmid_Backbone	High copy BioBrick assembly plasmid	Austin Che	2070
			Oxytocin-NeurophysinI_pUC57	Coding	Protein hormone and carrier molecule for expression in pUC57 plasmid	Lethbridge High School iGEM	406
			NECI_pUC57	Coding	Cleavage enzyme for expression in pUC57 plasmid	Lethbridge High School iGEM	2277
	★		BBa_J06702	Reporter	mCherry, bacterial with RBS and forward terminator	Yves Wang	869

Final Constructs:

Name	Description	Expanded Length	Total Length
K314100+oxytocin-neurophysin1 in pSB1C3	BBa_K314100 (J23100 promoter and B0034 RBS) and oxytocin-neurophysin1 gene in pSB1C3 plasmid	518 + 406 + 2070	2994
K314100+NEC1 in pSB1C3	BBa_K314100 (J23100 promoter and B0034 RBS) and NEC1 enzyme in pSB1C3 plasmid	518 + 2277 + 2070	4865
J23100+J06702 in pSB1C3	BBa_J23100 (high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid	35 + 869 + 2070	2974
J23108+J06702 in pSB1C3	BBa_J23108 (medium-high constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid	35 + 869 + 2070	2974
J23113+J06702 in pSB1C3	BBa_J23113 (medium-low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid	35 + 869 + 2070	2974
J23114+J06702 in pSB1C3	BBa_J23114 (low constitutive promoter) and BBa_J06702 (mCherry) in pSB1C3 plasmid	35 + 869 + 2070	2974