"
Team:Lethbridge Canada/results
From 2013hs.igem.org
Revision as of 03:28, 21 June 2013 by Adam.christiansen (Talk | contribs)
| Lane | Contents | Volume |
|---|---|---|
| 1 | Protein Molecular Weight Marker | 20μL |
| 2 | Cbf5-Nop10-Gar1 | 20μL |
| 3 | Buffer A wash | 30μL |
| 4 | Buffer B wash | 30μL |
| 5 | Buffer E Wash | 30μL |
| 6 | S-30 s/N | 30μL |
| 7 | Buffer E – Concentrated Preprooxyphysin | 30μL |
To detect our preprooxyphysin proteins we did a Silver Stain. The reason for doing a Silver Stain is that it is 10x more sensitive than the standard Coomassie gel. In Lane 2, we did a control of Cbf5-Nop10-Gar1. This is actually three proteins. Because of their large size, Cbf5 and Nop10 are found at the top of Lane 2. However, Gar1 is smaller, only 7kDa, and is found to be at the bottom. It is outlined in red. Preprooxyphysin is roughly twice as large as Gar1 at 13kDa and is found to about twice as large as Gar1. It is outlined by orange.
