Team:Lethbridge Canada/results

From 2013hs.igem.org

(Difference between revisions)
Line 69: Line 69:
<div id="mainContent">
<div id="mainContent">
 +
<p class="padding_top_p">
<h1>Silver Stained Tris-Tricine PAGE</h1>
<h1>Silver Stained Tris-Tricine PAGE</h1>

Revision as of 05:04, 21 June 2013

Silver Stained Tris-Tricine PAGE

Lane Contents Volume
1 Protein Molecular Weight Marker 20μL
2 Cbf5-Nop10-Gar1 20μL
3 Buffer A wash 30μL
4 Buffer B wash 30μL
5 Buffer E Wash 30μL
6 S-30 s/N 30μL
7 Buffer E – Concentrated Preprooxyphysin 30μL
Gel Gel

To detect our prepro-oxyphysin proteins we did a Silver Stain 16.5% Tris-Tricine PAGE. The reason for doing a Silver Stain is that it is 10x more sensitive than the standard Coomassie stain. The tricine in the Tris-TRicine PAGE allows for better and more distinct separation of small proteins in the gel. In Lane 2, we did a control of Cbf5-Nop10-Gar1 proteins. Cbf5 is 43 kDa, Gar1 is 12.3 kDa and Nop10 is only 7.2 kDa. Nop 10 is outlined in red. Prepro-oxyphysin is slightly larger than Nop10 at 10kDa. The bands we suspect are prepro-oxyphysin are outlined by orange.