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Team:Lethbridge Canada/results
From 2013hs.igem.org
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<h1>Anti-His Slot Blot</h1> | <h1>Anti-His Slot Blot</h1> | ||
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Revision as of 20:34, 21 June 2013
Silver Stained Tris-Tricine PAGE
| Lane | Contents | Volume |
|---|---|---|
| 1 | Protein Molecular Weight Marker | 20μL |
| 2 | Cbf5-Nop10-Gar1 | 20μL |
| 3 | Buffer A wash | 30μL |
| 4 | Buffer B wash | 30μL |
| 5 | Buffer E Wash | 30μL |
| 6 | S-30 s/N | 30μL |
| 7 | Buffer E – Concentrated Preprooxyphysin | 30μL |
To detect our prepro-oxyphysin proteins we did a Silver Stain 16.5% Tris-Tricine PAGE. The reason for doing a Silver Stain is that it is 10x more sensitive than the standard Coomassie stain. The tricine in the Tris-TRicine PAGE allows for better and more distinct separation of small proteins in the gel. In Lane 2, we did a control of Cbf5-Nop10-Gar1 proteins. Cbf5 is 43 kDa, Gar1 is 12.3 kDa and Nop10 is only 7.2 kDa. Nop 10 is outlined in red. Prepro-oxyphysin is slightly larger than Nop10 at 10kDa. The bands we suspect are prepro-oxyphysin are outlined by orange.
Anti-His Slot Blot
1%Agarose Gel of K314100_OXT
