Team:Lethbridge Canada/notebook march

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<li class="navigation_project"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project" class="strict">Project</a>
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<li class="navigation_project"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Project</a>
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<ul>
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<li></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Description</a></li>
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                                                <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project#video_oxy">Visual Modeling</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/math">Math Model</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/results">Results</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/wikifreeze">Wikifreeze</a></li>
</ul>
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<li class="navigation_parts"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/parts">Parts</a>
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<li class="navigation_parts decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/parts" class="strict">Parts</a></li>
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<li class="navigation_notebook decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols" class="strict">Notebook</a>
<ul>
<ul>
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<li></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols#protocol_header">Protocols</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">Notebook: March</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april">Notebook: April</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may">Notebook: May</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june">Notebook: June</a></li>
</ul>
</ul>
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<li class="navigation_notebook"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols">Notebook</a></li>
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<li class="navigation_safety decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/safety" class="strict">Safety</a></li>
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<li class="navigation_safety"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/safety">Safety</a></li>
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<li class="navigation_outreach decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach" class="strict">Outreach</a>
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<li class="navigation_outreach"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach">Outreach</a></li>
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<ul>
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<li class="navigation_team"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team">Team</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach">Overview</a></li>
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<li class="navigation_sponsors"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/sponsors">Sponsors</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/presentations">Presentations</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/interviews">Interviews</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/videos">Videos</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/surveys">Parent Surveys</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/novel_study">Novel Study</a></li>
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</ul>
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<li class="navigation_team decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team" class="strict">Team</a>
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<ul>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_team">The Team</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_advisors">The Advisors</a></li>
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</ul>
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</li>
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<li class="navigation_sponsors decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/sponsors" class="strict">Sponsors</a></li>
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<div id="mainContent">
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<div id="notebook_header">
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<div id="notebook_links">
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<h1 id="notebook_links_header">Notebook Links</h1>
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<h1 id="notebook_links_header"> Notebook Links</h1>
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<ul id="notebook_march_links">
<ul id="notebook_march_links">
<li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">March</a></li>
<li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">March</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eighteen">May 17, 2013: Miniprep</a></li>
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eighteen">May 17, 2013: Miniprep</a></li>
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_nineteen">May 17, 2013: Glycerol Stock</a></li>
<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_nineteen">May 17, 2013: Glycerol Stock</a></li>
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                        <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_one">May 22, 2013: Ligation</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_two">May 22, 2013: Transformations</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_three">May 23, 2013: Transformations</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_four">May 23, 2013: Picked Oxytocin and Transformations</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_five">May 24, 2013: Glycerol Stock</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_six">May 24, 2013: Miniprep</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_seven">May 26, 2013: Miniprep</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_eight">May 26, 2013: Restriction Digestion</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_nine">May 26, 2013: Agarose gel of Digestion</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty">May 26, 2013: Digestion Protocol</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty_one">May 31, 2013: Agarose Gel</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty_two">May 31, 2013: Everything</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty_three">May 31, 2013: Everything</a></li>
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</ul>
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<ul id="notebook_june_links">
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<li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june">June</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_one">June 1, 2013: Restrictions</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_two">June 2, 2013: Transformation</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_three">June 4, 2013: Picked Colonies</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_four">June 10, 2013: Sent constructs for sequencing</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_five">June 13, 2013: Transformation of OXY into BL21</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_six">June 13, 2013: Restricted and Ligated and Transformed</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_seven">June 13, 2013: Picked Cells</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_eight">June 15, 2013: Ni-Sepharose Purification</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_nine">June 18, 2013: Tris-Tricine PAGE</a></li>
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<li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_ten">June 21, 2013: Anti-His Slot Blot</a></li>
</ul>
</ul>
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<div class="pull_banner_left">
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<img src="https://static.igem.org/mediawiki/2013hs/4/4d/Lethbridge_hs_igem_2013_banner_notebookmarch.png">
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<div id="notebook_body">
<div id="notebook_body">
Line 135: Line 188:
<li >Name: Marie C, Fiona S, Chris I, Katie T, Wesley M</li>
<li >Name: Marie C, Fiona S, Chris I, Katie T, Wesley M</li>
<li >Date: March 12, 2013</li>
<li >Date: March 12, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
<li >Antibiotic: Kan and Amp</li>
<li >Antibiotic: Kan and Amp</li>
Line 152: Line 205:
<li >Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H</li>
<li >Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H</li>
<li >Date: March 13, 2013</li>
<li >Date: March 13, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
<li >Antibiotic: Amp</li>
<li >Antibiotic: Amp</li>
Line 169: Line 222:
<li >Names: Mark H, Chris I, Elaine B, Dax L</li>
<li >Names: Mark H, Chris I, Elaine B, Dax L</li>
<li >Date: March 13, 2013</li>
<li >Date: March 13, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
-
<li >Miniprepped: 3-B0030, 3-B0031, 3-B0032,
+
<li >Mini prepped: 3-B0030, 3-B0031, 3-B0032,
2-J04500 and 3-R0010</li>
2-J04500 and 3-R0010</li>
<li >Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5</li>
<li >Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5</li>
Line 186: Line 239:
<li >Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S</li>
<li >Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S</li>
<li >Date: March 15, 2013</li>
<li >Date: March 15, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe use buffer 4 instead of buffer 10</li>
<li >Protocol: Ute Kothe use buffer 4 instead of buffer 10</li>
<li >Restricted: 6-B0030, 6-B0031, 6-B0032,
<li >Restricted: 6-B0030, 6-B0031, 6-B0032,
4-J04500 and 6-R0010</li>
4-J04500 and 6-R0010</li>
-
<li >Incubate 1 hour at 37˚ C</li>
+
<li >Incubate 1 hour at 37˚C</li>
<li >Stored freezer overnight</li>
<li >Stored freezer overnight</li>
</ul>
</ul>
Line 205: Line 258:
<li >Name: Patrick O</li>
<li >Name: Patrick O</li>
<li >Date: March 16, 2013</li>
<li >Date: March 16, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
-
<li >Ran 15 uL each of 3-B0030, 3-B0031, 3-B0032,
+
<li >Ran 15µL each of 3-B0030, 3-B0031, 3-B0032,
2-J04500 and 3-R0010 through 1% agarose gel</li>
2-J04500 and 3-R0010 through 1% agarose gel</li>
-
<li >Quantities: 12.5 uL DNA and 2.5 uL of 6x loading dye (15 uL total), 12.5 uL 1kb DNA ladder and 2.5 uL 6x loading dye (15 uL total) note: ladder difficult to see on gel picture, next time less DNA ladder</li>
+
<li >Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total)  
 +
note: ladder difficult to see on gel picture, next time less DNA ladder</li>
<ul>
<ul>
</div>
</div>
Line 320: Line 374:
<li >Names: Fiona S, Katie T, Chris I, Elaine B. </li>
<li >Names: Fiona S, Katie T, Chris I, Elaine B. </li>
<li >Date: March 18, 2013 </li>
<li >Date: March 18, 2013 </li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
-
<li >Ran 15 uL each of 3-B0030, 3-B0031, 3-B0032,
+
<li >Ran 15µL each of 3-B0030, 3-B0031, 3-B0032,
2-J04500 and 3-R0010 through 1% agarose gel</li>
2-J04500 and 3-R0010 through 1% agarose gel</li>
-
<li >Quantities: 12.5 uL DNA and 2.5 uL of 6x loading dye (15 uL total), 12.5 uL 1kb DNA ladder and 2.5 uL 6x loading dye (15 uL total)</li>
+
<li >Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total)</li>
-
<li ><img src="images/lethbridgehs2013-3-18-agarose-picture.gif" alt"Agrose Gel on March 18, 2013" width="400px" /></li>
+
<li ><img src="https://static.igem.org/mediawiki/2013hs/f/fc/Leth_hs_2013_agarose_picture_3_18_2013.gif" alt"Agrose Gel on March 18, 2013" width="400px" /></li>
</ul>
</ul>
</div>
</div>
Line 444: Line 498:
<li >Names: Fiona S, Chris I.</li>
<li >Names: Fiona S, Chris I.</li>
<li >Date: March 19, 2013 </li>
<li >Date: March 19, 2013 </li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
-
<li >Transformed 2 uL of J04650 pSB1AK3 from Spring 2012 Kit Plate 4 C12 into competent cells.</li>
+
<li >Transformed 2µL of J04650 pSB1AK3 from Spring 2012 Kit Plate 4 C12 into competent cells.</li>
-
<li >Quantities: 2 uL J04650 pSB1AK3, 200 uL LB media</li>
+
<li >Quantities: 2µL J04650 pSB1AK3, 200µL LB media</li>
</ul>
</ul>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
Line 460: Line 514:
<li >Names: Yoyo Y, Wesley M</li>
<li >Names: Yoyo Y, Wesley M</li>
<li >Date: March 20, 2013</li>
<li >Date: March 20, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
<li >Picked cells of J04650 from previous day’s transformation</li>
<li >Picked cells of J04650 from previous day’s transformation</li>
Line 479: Line 533:
<li >Name: Mark H</li>
<li >Name: Mark H</li>
<li >Date: March 21, 2013</li>
<li >Date: March 21, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
<li >Miniprepped 1-R0010 used 50uL of Elution Buffer</li>
<li >Miniprepped 1-R0010 used 50uL of Elution Buffer</li>
Line 495: Line 549:
<li >Name: Yoyo Y</li>
<li >Name: Yoyo Y</li>
<li >Date: March 27, 2013</li>
<li >Date: March 27, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
-
<li >Transformed 2uL of J04650, and B0015 into 25uL of DH5A cell</li>
+
<li >Transformed 2µL of J04650, and B0015 into 25µL of DH5α cell</li>
-
<li >Quantities: 2uL of J04650 and 2uL of B0015 added into 25uL of DH5A cells</li>
+
<li >Quantities: 2µL of J04650 and 2µL of B0015 added into 25µL of DH5α cells</li>
</ul>
</ul>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
Line 511: Line 565:
<li >Names: Marie C, Fiona S, Chris I</li>
<li >Names: Marie C, Fiona S, Chris I</li>
<li >Date: March 28, 2013</li>
<li >Date: March 28, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
-
<li >Transformed 2.5uL of J06702</li>
+
<li >Transformed 2.5µL of J06702</li>
<li >Plasmid: pSB1A2 </li>
<li >Plasmid: pSB1A2 </li>
-
<li >Quantities: 2.5uL of DNA into 100uL of pSB1A2</li>
+
<li >Quantities: 2.5µL of DNA into 100µL of pSB1A2</li>
</ul>
</ul>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
Line 528: Line 582:
<li >Names: Chris I, Fiona S, Marie C</li>
<li >Names: Chris I, Fiona S, Marie C</li>
<li >Date: March 28, 2013</li>
<li >Date: March 28, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
<li >Picked 3 colonies from March 19, J04650</li>
<li >Picked 3 colonies from March 19, J04650</li>
<li >Picked 1 colony from March 26, J04650</li>
<li >Picked 1 colony from March 26, J04650</li>
<li >Picked 1 colony from March 27, B0015</li>
<li >Picked 1 colony from March 27, B0015</li>
-
<li >5mL cultures with 5uL ampicillin.  </li>
+
<li >5mL cultures with 5µL ampicillin.  </li>
</ul>
</ul>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
Line 546: Line 600:
<li >Names: Chris I, Fiona S, Marie C</li>
<li >Names: Chris I, Fiona S, Marie C</li>
<li >Date: March 29, 2013</li>
<li >Date: March 29, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
-
<li >Strain: DH5A</li>
+
<li >Strain: DH5α</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
<li >Picked 2 colonies from March 28, J06702</li>
<li >Picked 2 colonies from March 28, J06702</li>
-
<li >5mL cultures with 5uL ampicillin</li>
+
<li >5mL cultures with 5µL ampicillin</li>
</ul>
</ul>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p>
Line 562: Line 616:
<li >Names: Chris I, Fiona S, Marie C</li>
<li >Names: Chris I, Fiona S, Marie C</li>
<li >Date: March 29, 2013</li>
<li >Date: March 29, 2013</li>
-
<li >Species: E. Coli</li>
+
<li >Species: E. coli</li>
<li >Strain: DH5A</li>
<li >Strain: DH5A</li>
<li >Protocol: Ute Kothe</li>
<li >Protocol: Ute Kothe</li>
Line 570: Line 624:
<li >Picked, J23114</li>
<li >Picked, J23114</li>
<li >Picked, J23100 </li>
<li >Picked, J23100 </li>
-
<li >5mL cultures with 5uL ampicillin</li>
+
<li >5mL cultures with 5µL ampicillin</li>
-
<li >Shaken overnight at 37 degrees Celsius</li>
+
<li >Shaken overnight at 37°C</li>
</ul>
</ul>
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Latest revision as of 03:48, 22 June 2013

Transformations

  • Name: Marie C, Fiona S, Chris I, Katie T, Wesley M
  • Date: March 12, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Antibiotic: Kan and Amp
  • Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3
  • Results: TBA

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Picking Cell Colonies (Cultures)

  • Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H
  • Date: March 13, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Antibiotic: Amp
  • Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3
  • Results: TBA

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Miniprep and Glycerol Stock

  • Names: Mark H, Chris I, Elaine B, Dax L
  • Date: March 13, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Mini prepped: 3-B0030, 3-B0031, 3-B0032, 2-J04500 and 3-R0010
  • Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5

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Restriction Digestion

  • Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S
  • Date: March 15, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe use buffer 4 instead of buffer 10
  • Restricted: 6-B0030, 6-B0031, 6-B0032, 4-J04500 and 6-R0010
  • Incubate 1 hour at 37˚C
  • Stored freezer overnight

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Agarose Gel

  • Name: Patrick O
  • Date: March 16, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Ran 15µL each of 3-B0030, 3-B0031, 3-B0032, 2-J04500 and 3-R0010 through 1% agarose gel
  • Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total) note: ladder difficult to see on gel picture, next time less DNA ladder
Lane # Contents Volume(µL)
1 Empty n/a
2 1kb DNA Ladder 15
3 BBa_B0030 15
4 BBa_B0030 15
5 BBa_B0030 15
6 BBa_B0031 15
7 BBa_B0031 15
8 BBa_B0031 15
9 BBa_B0032 15
10 BBa_B0032 15
11 BBa_B0032 15
12 BBa_R0010 15
13 BBa_R0010 15
14 BBa_R0010 15
15 BBa_J04500 15
16 BBa_J04500 15
17+ empty n/a

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Agarose Gel

  • Names: Fiona S, Katie T, Chris I, Elaine B.
  • Date: March 18, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Ran 15µL each of 3-B0030, 3-B0031, 3-B0032, 2-J04500 and 3-R0010 through 1% agarose gel
  • Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total)
  • Lane # Contents Volume(µL)
    1 Empty n/a
    2 1 kb DNA Ladder 6
    2 1 kb DNA Ladder 6
    3 BBa_B0030 15
    4 BBa_B0030 15
    5 BBa_B0030 15
    6 BBa_B0031 15
    7 BBa_B0031 15
    8 BBa_B0031 15
    9 BBa_B0032 15
    10 BBa_B0032 15
    11 BBa_B0032 15
    12 BBa_R0010 15
    13 BBa_R0010 15
    14 BBa_R0010 15
    15 BBa_J04500 15
    16 BBa_J04500 15
    17+ Empty n/a

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Transformations of J04650

  • Names: Fiona S, Chris I.
  • Date: March 19, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Transformed 2µL of J04650 pSB1AK3 from Spring 2012 Kit Plate 4 C12 into competent cells.
  • Quantities: 2µL J04650 pSB1AK3, 200µL LB media

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Picking Cells of J04650

  • Names: Yoyo Y, Wesley M
  • Date: March 20, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Picked cells of J04650 from previous day’s transformation
  • Colonies didn't form properly within either plate, cells were picked with a dab (single colonies)
  • Quantities: 3x5ml of media
  • Antibiotic: Ampicillin
  • Glycerol Stock: R0010 pSB1A2

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Miniprep and Glycerol Stock of R0010

  • Name: Mark H
  • Date: March 21, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Miniprepped 1-R0010 used 50uL of Elution Buffer
  • Glycerol Stocked 1-R0010 in G6

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Transformations J04650 and B0015

  • Name: Yoyo Y
  • Date: March 27, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Transformed 2µL of J04650, and B0015 into 25µL of DH5α cell
  • Quantities: 2µL of J04650 and 2µL of B0015 added into 25µL of DH5α cells

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Transformations

  • Names: Marie C, Fiona S, Chris I
  • Date: March 28, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Transformed 2.5µL of J06702
  • Plasmid: pSB1A2
  • Quantities: 2.5µL of DNA into 100µL of pSB1A2

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Picking Cells of J04650

  • Names: Chris I, Fiona S, Marie C
  • Date: March 28, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Picked 3 colonies from March 19, J04650
  • Picked 1 colony from March 26, J04650
  • Picked 1 colony from March 27, B0015
  • 5mL cultures with 5µL ampicillin.

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Picking Cells of J06702

  • Names: Chris I, Fiona S, Marie C
  • Date: March 29, 2013
  • Species: E. coli
  • Strain: DH5α
  • Protocol: Ute Kothe
  • Picked 2 colonies from March 28, J06702
  • 5mL cultures with 5µL ampicillin

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Picking Cells of -80 Glycerol Stock , J61002, J23113, J23108, J23111, J23100

  • Names: Chris I, Fiona S, Marie C
  • Date: March 29, 2013
  • Species: E. coli
  • Strain: DH5A
  • Protocol: Ute Kothe
  • Picked, J61002
  • Picked, J23113
  • Picked, J23108
  • Picked, J23114
  • Picked, J23100
  • 5mL cultures with 5µL ampicillin
  • Shaken overnight at 37°C

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