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Team:Lethbridge Canada/notebook march
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<span id="title_first">Lethbridge</span> | <span id="title_first">Lethbridge</span> | ||
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<ul> | <ul> | ||
| - | <li></li> | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project">Description</a></li> |
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/project#video_oxy">Visual Modeling</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/math">Math Model</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/results">Results</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/wikifreeze">Wikifreeze</a></li> | ||
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| - | <li class="navigation_parts"><a href=" | + | <li class="navigation_parts decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/parts" class="strict">Parts</a></li> |
| + | <li class="navigation_notebook decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols" class="strict">Notebook</a> | ||
<ul> | <ul> | ||
| - | <li></li> | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/protocols#protocol_header">Protocols</a></li> |
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">Notebook: March</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april">Notebook: April</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may">Notebook: May</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june">Notebook: June</a></li> | ||
</ul> | </ul> | ||
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| - | <li class=" | + | <li class="navigation_safety decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/safety" class="strict">Safety</a></li> |
| - | <li class=" | + | <li class="navigation_outreach decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach" class="strict">Outreach</a> |
| - | + | <ul> | |
| - | <li class="navigation_team"><a href="team. | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/outreach">Overview</a></li> |
| - | <li class=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/presentations">Presentations</a></li> |
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/interviews">Interviews</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/videos">Videos</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/surveys">Parent Surveys</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/novel_study">Novel Study</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="navigation_team decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team" class="strict">Team</a> | ||
| + | <ul> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_team">The Team</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/team#the_advisors">The Advisors</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="navigation_sponsors decrease_opacity"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/sponsors" class="strict">Sponsors</a></li> | ||
</ul> | </ul> | ||
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</div> | </div> | ||
| - | + | ||
| - | + | ||
<div id="mainContent"> | <div id="mainContent"> | ||
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<div id="notebook_header"> | <div id="notebook_header"> | ||
<div id="notebook_links"> | <div id="notebook_links"> | ||
| - | <h1 id="notebook_links_header">Notebook Links</h1> | + | |
| + | <h1 id="notebook_links_header"> Notebook Links</h1> | ||
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<ul id="notebook_march_links"> | <ul id="notebook_march_links"> | ||
| - | <li class="notebook_link_title"><a href=" | + | <li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march">March</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_one">March 12, 2013: Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_two">March 13, 2013: Picking Cell Colonies (Cultures)</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_three">March 13, 2013: Miniprep and Glycerol Stock</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_four">March 15, 2013: Restriction Digestion</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_five">March 16, 2013: Agarose Gel</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_six">March 18, 2013: Agarose Gel</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_seven">March 19, 2013: Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_eight">March 20, 2013: Picking Cell Colonies (Cultures)</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_nine">March 21, 2013: Miniprep and Glycerol Stock</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_ten">March 27, 2013: Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_eleven">March 28, 2013: Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_twelve">March 28, 2013: Picking Cell Colonies (Cultures)</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_thirteen">March 29, 2013: Picking Cell Colonies (Cultures)</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_march#entry_fourteen">March 29, 2013: Picking Cells of -80 Glycerol Stock</a></li> |
</ul> | </ul> | ||
<ul id="notebook_april_links"> | <ul id="notebook_april_links"> | ||
| - | <li class="notebook_link_title"><a href=" | + | <li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april">April</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_one">April 2, 2013: Restriction - Ligation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_two">April 2, 2013: Ligation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_three">April 3, 2013: Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_four">April 4, 2013: Re-Plating Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_five">April 5, 2013: Miniprep of ON Cultures</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_six">April 5, 2013: UV Spectroscopy DNA Concentrations</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_seven">April 6, 2013: Miniprep From Ligations</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_eight">April 7, 2013: PCR</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_nine">April 8, 2013: Restriction Digestion of PCR Parts</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_ten">April 8, 2013: 3% Agarose Gel of Digested PCR Parts</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_eleven">April 8, 2013: Gel Extraction</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twelve">April 9, 2013: Repeat PCR</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_thirteen">April 9, 2013: Picking Cell Colonies (Cultures)</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_fourteen">April 10, 2013: Miniprep</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_fifteen">April 10, 2013: Restriction</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_sixteen">April 10, 2013: Agarose Gel of PCR 1%</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_seventeen">April 11, 2013: Miniprep</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_eighteen">April 12, 2013: Restriction Digestion</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_nineteen">April 12, 2013: PCR and Cell Reculturization</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty">April 12, 2013: Miniprep</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_one">April 14, 2013: Miniprep</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_two">April 14, 2013: 1% Agarose Gel</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_six">April 15, 2013: Restriction/Digestion</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_seven">April 15, 2013: Restriction/Digestion</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_three">April 15, 2013: 1% Agarose Gel of Promoters</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_four">April 17, 2013: Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_april#entry_twenty_five">April 30, 2013: 1% Agarose Gel of Promoter Constructs</a></li> |
</ul> | </ul> | ||
<ul id="notebook_may_links"> | <ul id="notebook_may_links"> | ||
| - | <li class="notebook_link_title"><a href=" | + | <li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may">May</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_one">May 1, 2013: Ligation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_two">May 4, 2013: Restriction Digest</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_three">May 4, 2013: 1% Agarose Gel of Promoter Constructs</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_four">May 4, 2013: Gel Extractions</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_five">May 5, 2013: Transformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_six">May 7, 2013: Restriction</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_seven">May 7, 2013: Gel Extraction</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eight">May 7, 2013: Gel Extracted on Conformation Gel</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_nine">May 8, 2013: Ligations</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_ten">May 9, 2013: PCR of Ligation Mixture</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eleven">May 13, 2013: Restriction of PCR Ligations Gel Conformation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twelve">May 14, 2013: Transformation of Ligations</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirteen">May 15, 2013: Ligation</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_fourteen">May 15, 2013: Picking Cells</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_fifteen">May 16, 2013: Picking Cells</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_sixteen">May 16, 2013: 10 Fold Miniprep</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_seventeen">May 17, 2013: Agarose Gel</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_eighteen">May 17, 2013: Miniprep</a></li> |
| - | <li><a href=" | + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_nineteen">May 17, 2013: Glycerol Stock</a></li> |
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_one">May 22, 2013: Ligation</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_two">May 22, 2013: Transformations</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_three">May 23, 2013: Transformations</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_four">May 23, 2013: Picked Oxytocin and Transformations</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_five">May 24, 2013: Glycerol Stock</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_six">May 24, 2013: Miniprep</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_seven">May 26, 2013: Miniprep</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_eight">May 26, 2013: Restriction Digestion</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_twenty_nine">May 26, 2013: Agarose gel of Digestion</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty">May 26, 2013: Digestion Protocol</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty_one">May 31, 2013: Agarose Gel</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty_two">May 31, 2013: Everything</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_may#entry_thirty_three">May 31, 2013: Everything</a></li> | ||
| + | </ul> | ||
| + | |||
| + | <ul id="notebook_june_links"> | ||
| + | <li class="notebook_link_title"><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june">June</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_one">June 1, 2013: Restrictions</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_two">June 2, 2013: Transformation</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_three">June 4, 2013: Picked Colonies</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_four">June 10, 2013: Sent constructs for sequencing</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_five">June 13, 2013: Transformation of OXY into BL21</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_six">June 13, 2013: Restricted and Ligated and Transformed</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_seven">June 13, 2013: Picked Cells</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_eight">June 15, 2013: Ni-Sepharose Purification</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_nine">June 18, 2013: Tris-Tricine PAGE</a></li> | ||
| + | <li><a href="https://2013hs.igem.org/Team:Lethbridge_Canada/notebook_june#entry_ten">June 21, 2013: Anti-His Slot Blot</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <div class="pull_banner_left"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013hs/4/4d/Lethbridge_hs_igem_2013_banner_notebookmarch.png"> | ||
| + | </div> | ||
<div id="notebook_body"> | <div id="notebook_body"> | ||
| Line 133: | Line 188: | ||
<li >Name: Marie C, Fiona S, Chris I, Katie T, Wesley M</li> | <li >Name: Marie C, Fiona S, Chris I, Katie T, Wesley M</li> | ||
<li >Date: March 12, 2013</li> | <li >Date: March 12, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
<li >Antibiotic: Kan and Amp</li> | <li >Antibiotic: Kan and Amp</li> | ||
| Line 150: | Line 205: | ||
<li >Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H</li> | <li >Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H</li> | ||
<li >Date: March 13, 2013</li> | <li >Date: March 13, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
<li >Antibiotic: Amp</li> | <li >Antibiotic: Amp</li> | ||
| Line 167: | Line 222: | ||
<li >Names: Mark H, Chris I, Elaine B, Dax L</li> | <li >Names: Mark H, Chris I, Elaine B, Dax L</li> | ||
<li >Date: March 13, 2013</li> | <li >Date: March 13, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
| - | <li > | + | <li >Mini prepped: 3-B0030, 3-B0031, 3-B0032, |
2-J04500 and 3-R0010</li> | 2-J04500 and 3-R0010</li> | ||
<li >Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5</li> | <li >Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5</li> | ||
| Line 184: | Line 239: | ||
<li >Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S</li> | <li >Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S</li> | ||
<li >Date: March 15, 2013</li> | <li >Date: March 15, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe use buffer 4 instead of buffer 10</li> | <li >Protocol: Ute Kothe use buffer 4 instead of buffer 10</li> | ||
<li >Restricted: 6-B0030, 6-B0031, 6-B0032, | <li >Restricted: 6-B0030, 6-B0031, 6-B0032, | ||
4-J04500 and 6-R0010</li> | 4-J04500 and 6-R0010</li> | ||
| - | <li >Incubate 1 hour at | + | <li >Incubate 1 hour at 37˚C</li> |
<li >Stored freezer overnight</li> | <li >Stored freezer overnight</li> | ||
</ul> | </ul> | ||
| Line 203: | Line 258: | ||
<li >Name: Patrick O</li> | <li >Name: Patrick O</li> | ||
<li >Date: March 16, 2013</li> | <li >Date: March 16, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
| - | <li >Ran | + | <li >Ran 15µL each of 3-B0030, 3-B0031, 3-B0032, |
2-J04500 and 3-R0010 through 1% agarose gel</li> | 2-J04500 and 3-R0010 through 1% agarose gel</li> | ||
| - | <li >Quantities: 12. | + | <li >Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total) |
| + | note: ladder difficult to see on gel picture, next time less DNA ladder</li> | ||
<ul> | <ul> | ||
</div> | </div> | ||
| Line 318: | Line 374: | ||
<li >Names: Fiona S, Katie T, Chris I, Elaine B. </li> | <li >Names: Fiona S, Katie T, Chris I, Elaine B. </li> | ||
<li >Date: March 18, 2013 </li> | <li >Date: March 18, 2013 </li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
| - | <li >Ran | + | <li >Ran 15µL each of 3-B0030, 3-B0031, 3-B0032, |
2-J04500 and 3-R0010 through 1% agarose gel</li> | 2-J04500 and 3-R0010 through 1% agarose gel</li> | ||
| - | <li >Quantities: 12. | + | <li >Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total)</li> |
| - | <li ><img src=" | + | <li ><img src="https://static.igem.org/mediawiki/2013hs/f/fc/Leth_hs_2013_agarose_picture_3_18_2013.gif" alt"Agrose Gel on March 18, 2013" width="400px" /></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 442: | Line 498: | ||
<li >Names: Fiona S, Chris I.</li> | <li >Names: Fiona S, Chris I.</li> | ||
<li >Date: March 19, 2013 </li> | <li >Date: March 19, 2013 </li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
| - | <li >Transformed | + | <li >Transformed 2µL of J04650 pSB1AK3 from Spring 2012 Kit Plate 4 C12 into competent cells.</li> |
| - | <li >Quantities: | + | <li >Quantities: 2µL J04650 pSB1AK3, 200µL LB media</li> |
</ul> | </ul> | ||
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | ||
| Line 458: | Line 514: | ||
<li >Names: Yoyo Y, Wesley M</li> | <li >Names: Yoyo Y, Wesley M</li> | ||
<li >Date: March 20, 2013</li> | <li >Date: March 20, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
<li >Picked cells of J04650 from previous day’s transformation</li> | <li >Picked cells of J04650 from previous day’s transformation</li> | ||
| Line 477: | Line 533: | ||
<li >Name: Mark H</li> | <li >Name: Mark H</li> | ||
<li >Date: March 21, 2013</li> | <li >Date: March 21, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
<li >Miniprepped 1-R0010 used 50uL of Elution Buffer</li> | <li >Miniprepped 1-R0010 used 50uL of Elution Buffer</li> | ||
| Line 493: | Line 549: | ||
<li >Name: Yoyo Y</li> | <li >Name: Yoyo Y</li> | ||
<li >Date: March 27, 2013</li> | <li >Date: March 27, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
| - | <li >Transformed | + | <li >Transformed 2µL of J04650, and B0015 into 25µL of DH5α cell</li> |
| - | <li >Quantities: | + | <li >Quantities: 2µL of J04650 and 2µL of B0015 added into 25µL of DH5α cells</li> |
</ul> | </ul> | ||
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | ||
| Line 509: | Line 565: | ||
<li >Names: Marie C, Fiona S, Chris I</li> | <li >Names: Marie C, Fiona S, Chris I</li> | ||
<li >Date: March 28, 2013</li> | <li >Date: March 28, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
| - | <li >Transformed 2. | + | <li >Transformed 2.5µL of J06702</li> |
<li >Plasmid: pSB1A2 </li> | <li >Plasmid: pSB1A2 </li> | ||
| - | <li >Quantities: 2. | + | <li >Quantities: 2.5µL of DNA into 100µL of pSB1A2</li> |
</ul> | </ul> | ||
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | ||
| Line 526: | Line 582: | ||
<li >Names: Chris I, Fiona S, Marie C</li> | <li >Names: Chris I, Fiona S, Marie C</li> | ||
<li >Date: March 28, 2013</li> | <li >Date: March 28, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
<li >Picked 3 colonies from March 19, J04650</li> | <li >Picked 3 colonies from March 19, J04650</li> | ||
<li >Picked 1 colony from March 26, J04650</li> | <li >Picked 1 colony from March 26, J04650</li> | ||
<li >Picked 1 colony from March 27, B0015</li> | <li >Picked 1 colony from March 27, B0015</li> | ||
| - | <li >5mL cultures with | + | <li >5mL cultures with 5µL ampicillin. </li> |
</ul> | </ul> | ||
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | ||
| Line 544: | Line 600: | ||
<li >Names: Chris I, Fiona S, Marie C</li> | <li >Names: Chris I, Fiona S, Marie C</li> | ||
<li >Date: March 29, 2013</li> | <li >Date: March 29, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
| - | <li >Strain: | + | <li >Strain: DH5α</li> |
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
<li >Picked 2 colonies from March 28, J06702</li> | <li >Picked 2 colonies from March 28, J06702</li> | ||
| - | <li >5mL cultures with | + | <li >5mL cultures with 5µL ampicillin</li> |
</ul> | </ul> | ||
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | ||
| Line 560: | Line 616: | ||
<li >Names: Chris I, Fiona S, Marie C</li> | <li >Names: Chris I, Fiona S, Marie C</li> | ||
<li >Date: March 29, 2013</li> | <li >Date: March 29, 2013</li> | ||
| - | <li >Species: E. | + | <li >Species: E. coli</li> |
<li >Strain: DH5A</li> | <li >Strain: DH5A</li> | ||
<li >Protocol: Ute Kothe</li> | <li >Protocol: Ute Kothe</li> | ||
| Line 568: | Line 624: | ||
<li >Picked, J23114</li> | <li >Picked, J23114</li> | ||
<li >Picked, J23100 </li> | <li >Picked, J23100 </li> | ||
| - | <li >5mL cultures with | + | <li >5mL cultures with 5µL ampicillin</li> |
| - | <li >Shaken overnight at | + | <li >Shaken overnight at 37°C</li> |
</ul> | </ul> | ||
<p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | <p class="top_notebook"><a href="#wrapper">Back to Top</a></p> | ||
Latest revision as of 03:48, 22 June 2013
Notebook Links
- March
- March 12, 2013: Transformation
- March 13, 2013: Picking Cell Colonies (Cultures)
- March 13, 2013: Miniprep and Glycerol Stock
- March 15, 2013: Restriction Digestion
- March 16, 2013: Agarose Gel
- March 18, 2013: Agarose Gel
- March 19, 2013: Transformation
- March 20, 2013: Picking Cell Colonies (Cultures)
- March 21, 2013: Miniprep and Glycerol Stock
- March 27, 2013: Transformation
- March 28, 2013: Transformation
- March 28, 2013: Picking Cell Colonies (Cultures)
- March 29, 2013: Picking Cell Colonies (Cultures)
- March 29, 2013: Picking Cells of -80 Glycerol Stock
- April
- April 2, 2013: Restriction - Ligation
- April 2, 2013: Ligation
- April 3, 2013: Transformation
- April 4, 2013: Re-Plating Transformation
- April 5, 2013: Miniprep of ON Cultures
- April 5, 2013: UV Spectroscopy DNA Concentrations
- April 6, 2013: Miniprep From Ligations
- April 7, 2013: PCR
- April 8, 2013: Restriction Digestion of PCR Parts
- April 8, 2013: 3% Agarose Gel of Digested PCR Parts
- April 8, 2013: Gel Extraction
- April 9, 2013: Repeat PCR
- April 9, 2013: Picking Cell Colonies (Cultures)
- April 10, 2013: Miniprep
- April 10, 2013: Restriction
- April 10, 2013: Agarose Gel of PCR 1%
- April 11, 2013: Miniprep
- April 12, 2013: Restriction Digestion
- April 12, 2013: PCR and Cell Reculturization
- April 12, 2013: Miniprep
- April 14, 2013: Miniprep
- April 14, 2013: 1% Agarose Gel
- April 15, 2013: Restriction/Digestion
- April 15, 2013: Restriction/Digestion
- April 15, 2013: 1% Agarose Gel of Promoters
- April 17, 2013: Transformation
- April 30, 2013: 1% Agarose Gel of Promoter Constructs
- May
- May 1, 2013: Ligation
- May 4, 2013: Restriction Digest
- May 4, 2013: 1% Agarose Gel of Promoter Constructs
- May 4, 2013: Gel Extractions
- May 5, 2013: Transformation
- May 7, 2013: Restriction
- May 7, 2013: Gel Extraction
- May 7, 2013: Gel Extracted on Conformation Gel
- May 8, 2013: Ligations
- May 9, 2013: PCR of Ligation Mixture
- May 13, 2013: Restriction of PCR Ligations Gel Conformation
- May 14, 2013: Transformation of Ligations
- May 15, 2013: Ligation
- May 15, 2013: Picking Cells
- May 16, 2013: Picking Cells
- May 16, 2013: 10 Fold Miniprep
- May 17, 2013: Agarose Gel
- May 17, 2013: Miniprep
- May 17, 2013: Glycerol Stock
- May 22, 2013: Ligation
- May 22, 2013: Transformations
- May 23, 2013: Transformations
- May 23, 2013: Picked Oxytocin and Transformations
- May 24, 2013: Glycerol Stock
- May 24, 2013: Miniprep
- May 26, 2013: Miniprep
- May 26, 2013: Restriction Digestion
- May 26, 2013: Agarose gel of Digestion
- May 26, 2013: Digestion Protocol
- May 31, 2013: Agarose Gel
- May 31, 2013: Everything
- May 31, 2013: Everything
- June
- June 1, 2013: Restrictions
- June 2, 2013: Transformation
- June 4, 2013: Picked Colonies
- June 10, 2013: Sent constructs for sequencing
- June 13, 2013: Transformation of OXY into BL21
- June 13, 2013: Restricted and Ligated and Transformed
- June 13, 2013: Picked Cells
- June 15, 2013: Ni-Sepharose Purification
- June 18, 2013: Tris-Tricine PAGE
- June 21, 2013: Anti-His Slot Blot
Transformations
- Name: Marie C, Fiona S, Chris I, Katie T, Wesley M
- Date: March 12, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Antibiotic: Kan and Amp
- Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3
- Results: TBA
Picking Cell Colonies (Cultures)
- Names: Thomas K, Carissa K, Dylan S, Mark H, Brianna C, Wesley M, Austin K, Kieran Mc, Brooke H
- Date: March 13, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Antibiotic: Amp
- Part Number and Plasmid: R0010 PSB1A2, B0032 PSB1A2, J04650 PSB1AK3, B0031 PSB1A2, B0030 PSB1A2, J04500 PSB1AK3
- Results: TBA
Miniprep and Glycerol Stock
- Names: Mark H, Chris I, Elaine B, Dax L
- Date: March 13, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Mini prepped: 3-B0030, 3-B0031, 3-B0032, 2-J04500 and 3-R0010
- Glycerol Stocked B0030 in G1, B0031 in G2, B0032 in G3, J04500 in G4 and R0010 in G5
Restriction Digestion
- Names: Patrick O, Steven T, Amrinder G, Yoyo Y, Wesley M, Orion S
- Date: March 15, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe use buffer 4 instead of buffer 10
- Restricted: 6-B0030, 6-B0031, 6-B0032, 4-J04500 and 6-R0010
- Incubate 1 hour at 37˚C
- Stored freezer overnight
Agarose Gel
- Name: Patrick O
- Date: March 16, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Ran 15µL each of 3-B0030, 3-B0031, 3-B0032, 2-J04500 and 3-R0010 through 1% agarose gel
- Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total) note: ladder difficult to see on gel picture, next time less DNA ladder
| Lane # | Contents | Volume(µL) |
|---|---|---|
| 1 | Empty | n/a |
| 2 | 1kb DNA Ladder | 15 |
| 3 | BBa_B0030 | 15 |
| 4 | BBa_B0030 | 15 |
| 5 | BBa_B0030 | 15 |
| 6 | BBa_B0031 | 15 |
| 7 | BBa_B0031 | 15 |
| 8 | BBa_B0031 | 15 |
| 9 | BBa_B0032 | 15 |
| 10 | BBa_B0032 | 15 |
| 11 | BBa_B0032 | 15 |
| 12 | BBa_R0010 | 15 |
| 13 | BBa_R0010 | 15 |
| 14 | BBa_R0010 | 15 |
| 15 | BBa_J04500 | 15 |
| 16 | BBa_J04500 | 15 |
| 17+ | empty | n/a |
Agarose Gel
- Names: Fiona S, Katie T, Chris I, Elaine B.
- Date: March 18, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Ran 15µL each of 3-B0030, 3-B0031, 3-B0032, 2-J04500 and 3-R0010 through 1% agarose gel
- Quantities: 12.5µL DNA and 2.5µL of 6x loading dye (15µL total), 12.5µL 1kb DNA ladder and 2.5µL 6x loading dye (15µL total)
-
Lane # Contents Volume(µL) 1 Empty n/a 2 1 kb DNA Ladder 6 2 1 kb DNA Ladder 6 3 BBa_B0030 15 4 BBa_B0030 15 5 BBa_B0030 15 6 BBa_B0031 15 7 BBa_B0031 15 8 BBa_B0031 15 9 BBa_B0032 15 10 BBa_B0032 15 11 BBa_B0032 15 12 BBa_R0010 15 13 BBa_R0010 15 14 BBa_R0010 15 15 BBa_J04500 15 16 BBa_J04500 15 17+ Empty n/a
Transformations of J04650
- Names: Fiona S, Chris I.
- Date: March 19, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Transformed 2µL of J04650 pSB1AK3 from Spring 2012 Kit Plate 4 C12 into competent cells.
- Quantities: 2µL J04650 pSB1AK3, 200µL LB media
Picking Cells of J04650
- Names: Yoyo Y, Wesley M
- Date: March 20, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Picked cells of J04650 from previous day’s transformation
- Colonies didn't form properly within either plate, cells were picked with a dab (single colonies)
- Quantities: 3x5ml of media
- Antibiotic: Ampicillin
- Glycerol Stock: R0010 pSB1A2
Miniprep and Glycerol Stock of R0010
- Name: Mark H
- Date: March 21, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Miniprepped 1-R0010 used 50uL of Elution Buffer
- Glycerol Stocked 1-R0010 in G6
Transformations J04650 and B0015
- Name: Yoyo Y
- Date: March 27, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Transformed 2µL of J04650, and B0015 into 25µL of DH5α cell
- Quantities: 2µL of J04650 and 2µL of B0015 added into 25µL of DH5α cells
Transformations
- Names: Marie C, Fiona S, Chris I
- Date: March 28, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Transformed 2.5µL of J06702
- Plasmid: pSB1A2
- Quantities: 2.5µL of DNA into 100µL of pSB1A2
Picking Cells of J04650
- Names: Chris I, Fiona S, Marie C
- Date: March 28, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Picked 3 colonies from March 19, J04650
- Picked 1 colony from March 26, J04650
- Picked 1 colony from March 27, B0015
- 5mL cultures with 5µL ampicillin.
Picking Cells of J06702
- Names: Chris I, Fiona S, Marie C
- Date: March 29, 2013
- Species: E. coli
- Strain: DH5α
- Protocol: Ute Kothe
- Picked 2 colonies from March 28, J06702
- 5mL cultures with 5µL ampicillin
Picking Cells of -80 Glycerol Stock , J61002, J23113, J23108, J23111, J23100
- Names: Chris I, Fiona S, Marie C
- Date: March 29, 2013
- Species: E. coli
- Strain: DH5A
- Protocol: Ute Kothe
- Picked, J61002
- Picked, J23113
- Picked, J23108
- Picked, J23114
- Picked, J23100
- 5mL cultures with 5µL ampicillin
- Shaken overnight at 37°C
